Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and hisG are separated by 3 nucleotides hisG and SO2075 are separated by 586 nucleotides
SO2073: hisD - histidinol dehydrogenase (NCBI ptt file), at 2,169,632 to 2,170,948
hisD
SO2074: hisG - ATP phosphoribosyltransferase (NCBI ptt file), at 2,170,952 to 2,171,926
hisG
SO2075: SO2075 - hypothetical protein (NCBI ptt file), at 2,172,513 to 2,173,340
SO2075
Position (kb)
2170
2171
2172 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2169.960 kb on - strand, within hisD at 2170.143 kb on - strand, within hisD at 2170.148 kb on - strand, within hisD at 2170.173 kb on + strand at 2170.208 kb on + strand, within hisD at 2170.216 kb on - strand, within hisD at 2170.254 kb on - strand, within hisD at 2170.370 kb on - strand, within hisD at 2170.476 kb on - strand, within hisD at 2170.496 kb on + strand, within hisD at 2170.510 kb on - strand, within hisD at 2170.771 kb on + strand, within hisD at 2170.885 kb on - strand at 2170.984 kb on + strand at 2171.004 kb on + strand at 2171.014 kb on + strand at 2171.014 kb on + strand at 2171.046 kb on - strand at 2171.207 kb on + strand, within hisG at 2171.257 kb on - strand, within hisG at 2171.293 kb on + strand, within hisG at 2171.360 kb on + strand, within hisG at 2171.526 kb on + strand, within hisG at 2171.534 kb on - strand, within hisG at 2171.595 kb on - strand, within hisG at 2171.785 kb on + strand, within hisG at 2171.862 kb on + strand at 2172.043 kb on + strand at 2172.128 kb on - strand at 2172.163 kb on - strand at 2172.204 kb on - strand at 2172.281 kb on - strand at 2172.436 kb on - strand at 2172.461 kb on + strand at 2172.461 kb on + strand at 2172.467 kb on + strand at 2172.467 kb on + strand at 2172.467 kb on + strand at 2172.467 kb on + strand at 2172.467 kb on + strand at 2172.467 kb on + strand at 2172.469 kb on + strand at 2172.469 kb on - strand at 2172.475 kb on - strand at 2172.475 kb on - strand at 2172.510 kb on + strand at 2172.539 kb on + strand at 2172.548 kb on - strand at 2172.570 kb on - strand at 2172.577 kb on + strand at 2172.585 kb on - strand at 2172.615 kb on + strand, within SO2075 at 2172.618 kb on - strand, within SO2075 at 2172.696 kb on - strand, within SO2075 at 2172.711 kb on + strand, within SO2075 at 2172.730 kb on + strand, within SO2075 at 2172.736 kb on + strand, within SO2075 at 2172.738 kb on - strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.749 kb on + strand, within SO2075 at 2172.752 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.757 kb on - strand, within SO2075 at 2172.779 kb on + strand, within SO2075 at 2172.779 kb on + strand, within SO2075 at 2172.779 kb on + strand, within SO2075 at 2172.779 kb on + strand, within SO2075 at 2172.779 kb on + strand, within SO2075 at 2172.787 kb on - strand, within SO2075 at 2172.787 kb on - strand, within SO2075 at 2172.787 kb on - strand, within SO2075 at 2172.787 kb on - strand, within SO2075 at 2172.787 kb on - strand, within SO2075 at 2172.787 kb on - strand, within SO2075 at 2172.787 kb on - strand, within SO2075 at 2172.793 kb on + strand, within SO2075 at 2172.810 kb on - strand, within SO2075 at 2172.890 kb on - strand, within SO2075 at 2172.896 kb on + strand, within SO2075 at 2172.920 kb on - strand, within SO2075
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 2,169,960 - hisD SO2073 0.25 -3.0 2,170,143 - hisD SO2073 0.39 -2.6 2,170,148 - hisD SO2073 0.39 -5.0 2,170,173 + -1.2 2,170,208 + hisD SO2073 0.44 -5.2 2,170,216 - hisD SO2073 0.44 -3.5 2,170,254 - hisD SO2073 0.47 -4.8 2,170,370 - hisD SO2073 0.56 -3.1 2,170,476 - hisD SO2073 0.64 -3.3 2,170,496 + hisD SO2073 0.66 -5.1 2,170,510 - hisD SO2073 0.67 -3.6 2,170,771 + hisD SO2073 0.86 -3.9 2,170,885 - -3.8 2,170,984 + -3.6 2,171,004 + -3.7 2,171,014 + -4.9 2,171,014 + -3.9 2,171,046 - +0.6 2,171,207 + hisG SO2074 0.26 -4.5 2,171,257 - hisG SO2074 0.31 -4.3 2,171,293 + hisG SO2074 0.35 -5.3 2,171,360 + hisG SO2074 0.42 -5.0 2,171,526 + hisG SO2074 0.59 -5.3 2,171,534 - hisG SO2074 0.60 -3.5 2,171,595 - hisG SO2074 0.66 -2.2 2,171,785 + hisG SO2074 0.85 -1.8 2,171,862 + -3.5 2,172,043 + -2.3 2,172,128 - -3.6 2,172,163 - -1.6 2,172,204 - -0.6 2,172,281 - +0.3 2,172,436 - -0.3 2,172,461 + +0.0 2,172,461 + -0.4 2,172,467 + +0.1 2,172,467 + -0.1 2,172,467 + +0.3 2,172,467 + -0.4 2,172,467 + -0.0 2,172,467 + +0.2 2,172,469 + -0.4 2,172,469 - -1.0 2,172,475 - +0.3 2,172,475 - +0.0 2,172,510 + +0.6 2,172,539 + +0.6 2,172,548 - -0.8 2,172,570 - +0.7 2,172,577 + +0.3 2,172,585 - -0.2 2,172,615 + SO2075 0.12 -0.2 2,172,618 - SO2075 0.13 -0.4 2,172,696 - SO2075 0.22 -0.3 2,172,711 + SO2075 0.24 -0.4 2,172,730 + SO2075 0.26 +0.2 2,172,736 + SO2075 0.27 -0.4 2,172,738 - SO2075 0.27 +0.6 2,172,749 + SO2075 0.29 -0.2 2,172,749 + SO2075 0.29 -0.1 2,172,749 + SO2075 0.29 +0.4 2,172,749 + SO2075 0.29 +1.2 2,172,749 + SO2075 0.29 -0.2 2,172,749 + SO2075 0.29 -0.3 2,172,749 + SO2075 0.29 -0.1 2,172,749 + SO2075 0.29 +0.4 2,172,749 + SO2075 0.29 -0.3 2,172,749 + SO2075 0.29 +0.1 2,172,749 + SO2075 0.29 +0.2 2,172,749 + SO2075 0.29 +1.7 2,172,749 + SO2075 0.29 -0.7 2,172,749 + SO2075 0.29 +0.1 2,172,749 + SO2075 0.29 -1.0 2,172,749 + SO2075 0.29 +1.0 2,172,749 + SO2075 0.29 +0.2 2,172,749 + SO2075 0.29 +0.3 2,172,749 + SO2075 0.29 +0.2 2,172,749 + SO2075 0.29 -0.8 2,172,749 + SO2075 0.29 -0.1 2,172,749 + SO2075 0.29 -0.4 2,172,749 + SO2075 0.29 -0.6 2,172,749 + SO2075 0.29 +1.0 2,172,749 + SO2075 0.29 +1.0 2,172,749 + SO2075 0.29 +0.2 2,172,749 + SO2075 0.29 -0.1 2,172,749 + SO2075 0.29 -1.3 2,172,749 + SO2075 0.29 +0.2 2,172,749 + SO2075 0.29 -1.1 2,172,749 + SO2075 0.29 +0.1 2,172,749 + SO2075 0.29 +0.7 2,172,749 + SO2075 0.29 +1.0 2,172,749 + SO2075 0.29 -0.4 2,172,752 - SO2075 0.29 -0.1 2,172,757 - SO2075 0.29 +1.2 2,172,757 - SO2075 0.29 +0.1 2,172,757 - SO2075 0.29 -0.4 2,172,757 - SO2075 0.29 +1.6 2,172,757 - SO2075 0.29 -0.3 2,172,757 - SO2075 0.29 -0.3 2,172,757 - SO2075 0.29 +0.1 2,172,757 - SO2075 0.29 -0.9 2,172,757 - SO2075 0.29 +0.3 2,172,757 - SO2075 0.29 +0.3 2,172,757 - SO2075 0.29 +0.2 2,172,757 - SO2075 0.29 -0.3 2,172,757 - SO2075 0.29 -0.0 2,172,757 - SO2075 0.29 -0.3 2,172,757 - SO2075 0.29 -0.3 2,172,757 - SO2075 0.29 +0.4 2,172,757 - SO2075 0.29 +0.0 2,172,757 - SO2075 0.29 +0.0 2,172,757 - SO2075 0.29 -0.2 2,172,757 - SO2075 0.29 -0.1 2,172,757 - SO2075 0.29 +0.1 2,172,757 - SO2075 0.29 -0.4 2,172,757 - SO2075 0.29 +0.1 2,172,757 - SO2075 0.29 -0.0 2,172,757 - SO2075 0.29 +0.0 2,172,757 - SO2075 0.29 +0.3 2,172,757 - SO2075 0.29 -0.2 2,172,757 - SO2075 0.29 -0.0 2,172,757 - SO2075 0.29 -0.3 2,172,757 - SO2075 0.29 +0.7 2,172,757 - SO2075 0.29 -0.3 2,172,757 - SO2075 0.29 +0.3 2,172,757 - SO2075 0.29 +0.2 2,172,757 - SO2075 0.29 +0.0 2,172,757 - SO2075 0.29 +0.1 2,172,757 - SO2075 0.29 -0.9 2,172,779 + SO2075 0.32 +0.1 2,172,779 + SO2075 0.32 +0.0 2,172,779 + SO2075 0.32 -0.1 2,172,779 + SO2075 0.32 -0.5 2,172,779 + SO2075 0.32 -0.5 2,172,787 - SO2075 0.33 +0.0 2,172,787 - SO2075 0.33 +0.1 2,172,787 - SO2075 0.33 +0.2 2,172,787 - SO2075 0.33 -1.0 2,172,787 - SO2075 0.33 +0.5 2,172,787 - SO2075 0.33 -0.1 2,172,787 - SO2075 0.33 -0.5 2,172,793 + SO2075 0.34 +0.2 2,172,810 - SO2075 0.36 +0.1 2,172,890 - SO2075 0.46 +0.4 2,172,896 + SO2075 0.46 +1.2 2,172,920 - SO2075 0.49 +1.2
Or see this region's nucleotide sequence