Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisI and hisF are separated by 82 nucleotides hisF and hisA overlap by 19 nucleotides hisA and hisH are separated by 104 nucleotides hisH and hisB are separated by 144 nucleotides
SO2067: hisI - phosphoribosyl-ATP pyrophosphatase/phosphoribosyl-AMP cyclohydrolase (NCBI ptt file), at 2,164,095 to 2,164,730
hisI
SO2068: hisF - hisF protein (cyclase) (NCBI ptt file), at 2,164,813 to 2,165,586
hisF
SO2069: hisA - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (NCBI ptt file), at 2,165,568 to 2,166,305
hisA
SO2070: hisH - amidotransferase HisH (NCBI ptt file), at 2,166,410 to 2,167,075
hisH
SO2071: hisB - imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase (NCBI ptt file), at 2,167,220 to 2,168,311
hisB
Position (kb)
2165
2166
2167 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 2164.600 kb on + strand, within hisI at 2164.800 kb on + strand at 2164.800 kb on + strand at 2164.851 kb on + strand at 2164.917 kb on - strand, within hisF at 2164.924 kb on + strand, within hisF at 2164.953 kb on + strand, within hisF at 2165.133 kb on - strand, within hisF at 2165.306 kb on - strand, within hisF at 2165.328 kb on - strand, within hisF at 2165.390 kb on + strand, within hisF at 2165.398 kb on - strand, within hisF at 2165.421 kb on - strand, within hisF at 2165.528 kb on + strand at 2165.529 kb on - strand at 2165.561 kb on - strand at 2165.583 kb on + strand at 2165.602 kb on + strand at 2165.660 kb on + strand, within hisA at 2165.695 kb on - strand, within hisA at 2165.746 kb on - strand, within hisA at 2165.799 kb on - strand, within hisA at 2165.924 kb on + strand, within hisA at 2166.092 kb on + strand, within hisA at 2166.092 kb on + strand, within hisA at 2166.100 kb on - strand, within hisA at 2166.100 kb on - strand, within hisA at 2166.216 kb on + strand, within hisA at 2166.241 kb on + strand at 2166.311 kb on - strand at 2166.483 kb on + strand, within hisH at 2166.483 kb on + strand, within hisH at 2166.510 kb on + strand, within hisH at 2166.552 kb on - strand, within hisH at 2166.575 kb on - strand, within hisH at 2166.656 kb on + strand, within hisH at 2166.792 kb on + strand, within hisH at 2166.942 kb on + strand, within hisH at 2166.943 kb on + strand, within hisH at 2167.012 kb on + strand at 2167.130 kb on - strand at 2167.185 kb on + strand at 2167.193 kb on - strand at 2167.193 kb on - strand at 2167.225 kb on - strand at 2167.294 kb on - strand at 2167.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 2,164,600 + hisI SO2067 0.79 -3.9 2,164,800 + -3.3 2,164,800 + -4.3 2,164,851 + -5.5 2,164,917 - hisF SO2068 0.13 -6.5 2,164,924 + hisF SO2068 0.14 -4.8 2,164,953 + hisF SO2068 0.18 -2.3 2,165,133 - hisF SO2068 0.41 -4.3 2,165,306 - hisF SO2068 0.64 -4.4 2,165,328 - hisF SO2068 0.67 -4.1 2,165,390 + hisF SO2068 0.75 -5.1 2,165,398 - hisF SO2068 0.76 -2.0 2,165,421 - hisF SO2068 0.79 -2.2 2,165,528 + -5.7 2,165,529 - -4.7 2,165,561 - -2.2 2,165,583 + -1.8 2,165,602 + -4.0 2,165,660 + hisA SO2069 0.12 -2.9 2,165,695 - hisA SO2069 0.17 -1.8 2,165,746 - hisA SO2069 0.24 -4.0 2,165,799 - hisA SO2069 0.31 -2.6 2,165,924 + hisA SO2069 0.48 -4.3 2,166,092 + hisA SO2069 0.71 -3.8 2,166,092 + hisA SO2069 0.71 -2.6 2,166,100 - hisA SO2069 0.72 -5.6 2,166,100 - hisA SO2069 0.72 -4.8 2,166,216 + hisA SO2069 0.88 -2.6 2,166,241 + -0.2 2,166,311 - -3.1 2,166,483 + hisH SO2070 0.11 -3.1 2,166,483 + hisH SO2070 0.11 -4.6 2,166,510 + hisH SO2070 0.15 -5.2 2,166,552 - hisH SO2070 0.21 -4.6 2,166,575 - hisH SO2070 0.25 -4.3 2,166,656 + hisH SO2070 0.37 -4.9 2,166,792 + hisH SO2070 0.57 -4.4 2,166,942 + hisH SO2070 0.80 -4.0 2,166,943 + hisH SO2070 0.80 -4.4 2,167,012 + -7.6 2,167,130 - -4.1 2,167,185 + -5.5 2,167,193 - +0.1 2,167,193 - +0.6 2,167,225 - +0.9 2,167,294 - -3.1 2,167,305 + -3.5
Or see this region's nucleotide sequence