Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0476

Experiment: Benzalkonium Chloride 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0475 and Psest_0476 are separated by 196 nucleotidesPsest_0476 and Psest_0477 are separated by 382 nucleotides Psest_0475: Psest_0475 - 3-deoxy-D-manno-octulosonic-acid transferase, at 523,442 to 524,710 _0475 Psest_0476: Psest_0476 - type I secretion outer membrane protein, TolC family, at 524,907 to 526,361 _0476 Psest_0477: Psest_0477 - thiamine biosynthesis protein ThiC, at 526,744 to 528,624 _0477 Position (kb) 524 525 526 527Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 524.796 kb on + strandat 524.827 kb on + strandat 524.830 kb on + strandat 524.830 kb on + strandat 524.831 kb on - strandat 524.831 kb on - strandat 524.831 kb on - strandat 524.864 kb on + strandat 524.864 kb on + strandat 524.865 kb on - strandat 524.865 kb on - strandat 524.980 kb on + strandat 524.980 kb on + strandat 524.980 kb on + strandat 525.070 kb on + strand, within Psest_0476at 525.070 kb on + strand, within Psest_0476at 525.070 kb on + strand, within Psest_0476at 525.071 kb on - strand, within Psest_0476at 525.071 kb on - strand, within Psest_0476at 525.071 kb on - strand, within Psest_0476at 525.071 kb on - strand, within Psest_0476at 525.071 kb on - strand, within Psest_0476at 525.071 kb on - strand, within Psest_0476at 525.091 kb on + strand, within Psest_0476at 525.091 kb on + strand, within Psest_0476at 525.091 kb on + strand, within Psest_0476at 525.092 kb on - strand, within Psest_0476at 525.092 kb on - strand, within Psest_0476at 525.132 kb on + strand, within Psest_0476at 525.132 kb on + strand, within Psest_0476at 525.328 kb on + strand, within Psest_0476at 525.331 kb on + strand, within Psest_0476at 525.331 kb on + strand, within Psest_0476at 525.332 kb on - strand, within Psest_0476at 525.332 kb on - strand, within Psest_0476at 525.370 kb on + strand, within Psest_0476at 525.371 kb on - strand, within Psest_0476at 525.371 kb on - strand, within Psest_0476at 525.575 kb on - strand, within Psest_0476at 525.691 kb on + strand, within Psest_0476at 525.691 kb on + strand, within Psest_0476at 525.691 kb on + strand, within Psest_0476at 525.692 kb on - strand, within Psest_0476at 525.692 kb on - strand, within Psest_0476at 525.883 kb on - strand, within Psest_0476at 525.922 kb on + strand, within Psest_0476at 525.923 kb on - strand, within Psest_0476at 526.252 kb on + strandat 526.252 kb on + strandat 526.379 kb on + strandat 526.380 kb on - strandat 526.380 kb on - strandat 526.526 kb on + strandat 526.730 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.751 kb on + strandat 526.752 kb on - strandat 526.828 kb on + strandat 526.828 kb on + strandat 526.951 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.979 kb on + strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 526.980 kb on - strand, within Psest_0477at 527.203 kb on + strand, within Psest_0477at 527.203 kb on + strand, within Psest_0477at 527.203 kb on + strand, within Psest_0477at 527.203 kb on + strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.204 kb on - strand, within Psest_0477at 527.280 kb on + strand, within Psest_0477at 527.280 kb on + strand, within Psest_0477at 527.281 kb on - strand, within Psest_0477

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.002 mg/ml
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524,796 + +0.4
524,827 + +0.2
524,830 + +2.0
524,830 + +0.0
524,831 - -0.2
524,831 - -1.0
524,831 - -0.3
524,864 + +0.0
524,864 + +2.0
524,865 - -0.2
524,865 - +0.7
524,980 + -2.7
524,980 + -0.8
524,980 + -1.3
525,070 + Psest_0476 0.11 -2.9
525,070 + Psest_0476 0.11 -0.6
525,070 + Psest_0476 0.11 +0.4
525,071 - Psest_0476 0.11 -0.6
525,071 - Psest_0476 0.11 -2.7
525,071 - Psest_0476 0.11 -1.3
525,071 - Psest_0476 0.11 -3.2
525,071 - Psest_0476 0.11 -2.4
525,071 - Psest_0476 0.11 -1.2
525,091 + Psest_0476 0.13 -2.0
525,091 + Psest_0476 0.13 -3.2
525,091 + Psest_0476 0.13 -2.5
525,092 - Psest_0476 0.13 -2.5
525,092 - Psest_0476 0.13 -2.7
525,132 + Psest_0476 0.15 -3.8
525,132 + Psest_0476 0.15 -1.3
525,328 + Psest_0476 0.29 -1.8
525,331 + Psest_0476 0.29 -4.1
525,331 + Psest_0476 0.29 -2.7
525,332 - Psest_0476 0.29 -0.3
525,332 - Psest_0476 0.29 -1.0
525,370 + Psest_0476 0.32 -2.8
525,371 - Psest_0476 0.32 -3.2
525,371 - Psest_0476 0.32 -1.8
525,575 - Psest_0476 0.46 -2.2
525,691 + Psest_0476 0.54 -2.4
525,691 + Psest_0476 0.54 -2.3
525,691 + Psest_0476 0.54 -0.8
525,692 - Psest_0476 0.54 -1.8
525,692 - Psest_0476 0.54 -3.4
525,883 - Psest_0476 0.67 -2.0
525,922 + Psest_0476 0.70 -2.8
525,923 - Psest_0476 0.70 -3.4
526,252 + -3.5
526,252 + -5.4
526,379 + -4.6
526,380 - +0.2
526,380 - -2.7
526,526 + +0.5
526,730 + +0.0
526,751 + +0.0
526,751 + +1.0
526,751 + -0.8
526,751 + -0.4
526,751 + -0.5
526,751 + +0.1
526,751 + -1.0
526,752 - -0.6
526,828 + +1.9
526,828 + +1.0
526,951 + Psest_0477 0.11 +0.6
526,979 + Psest_0477 0.12 -0.6
526,979 + Psest_0477 0.12 +2.2
526,979 + Psest_0477 0.12 -0.9
526,979 + Psest_0477 0.12 -2.5
526,979 + Psest_0477 0.12 -0.1
526,979 + Psest_0477 0.12 -1.6
526,980 - Psest_0477 0.13 +0.5
526,980 - Psest_0477 0.13 -0.3
526,980 - Psest_0477 0.13 -1.0
526,980 - Psest_0477 0.13 +0.2
526,980 - Psest_0477 0.13 -0.6
526,980 - Psest_0477 0.13 +0.5
526,980 - Psest_0477 0.13 -0.4
526,980 - Psest_0477 0.13 -0.7
526,980 - Psest_0477 0.13 +0.2
526,980 - Psest_0477 0.13 -0.3
526,980 - Psest_0477 0.13 -0.2
527,203 + Psest_0477 0.24 -0.6
527,203 + Psest_0477 0.24 -0.7
527,203 + Psest_0477 0.24 -2.9
527,203 + Psest_0477 0.24 -2.2
527,204 - Psest_0477 0.24 +0.8
527,204 - Psest_0477 0.24 +0.4
527,204 - Psest_0477 0.24 +0.8
527,204 - Psest_0477 0.24 +1.0
527,204 - Psest_0477 0.24 +1.3
527,204 - Psest_0477 0.24 +1.3
527,204 - Psest_0477 0.24 -0.2
527,280 + Psest_0477 0.28 +0.6
527,280 + Psest_0477 0.28 +0.1
527,281 - Psest_0477 0.29 +0.6

Or see this region's nucleotide sequence