Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1495

Experiment: Benzalkonium Chloride 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1494 and Psest_1495 are separated by 155 nucleotidesPsest_1495 and Psest_1496 are separated by 73 nucleotidesPsest_1496 and Psest_1497 are separated by 3 nucleotides Psest_1494: Psest_1494 - DNA-binding regulatory protein, YebC/PmpR family, at 1,596,654 to 1,597,403 _1494 Psest_1495: Psest_1495 - crossover junction endodeoxyribonuclease RuvC, at 1,597,559 to 1,598,083 _1495 Psest_1496: Psest_1496 - Holliday junction DNA helicase, RuvA subunit, at 1,598,157 to 1,598,762 _1496 Psest_1497: Psest_1497 - Holliday junction DNA helicase, RuvB subunit, at 1,598,766 to 1,599,818 _1497 Position (kb) 1597 1598 1599Strain fitness (log2 ratio) -3 -2 -1 0 1at 1597.435 kb on + strandat 1597.435 kb on + strandat 1597.435 kb on + strandat 1597.436 kb on - strandat 1597.520 kb on + strandat 1597.521 kb on - strandat 1597.537 kb on - strandat 1597.540 kb on + strandat 1597.540 kb on + strandat 1598.113 kb on - strandat 1598.332 kb on + strand, within Psest_1496at 1598.333 kb on - strand, within Psest_1496at 1598.333 kb on - strand, within Psest_1496at 1598.760 kb on + strandat 1598.760 kb on + strandat 1598.760 kb on + strandat 1598.760 kb on + strandat 1598.761 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.002 mg/ml
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1,597,435 + +0.2
1,597,435 + +0.5
1,597,435 + -0.4
1,597,436 - -0.3
1,597,520 + +0.4
1,597,521 - -2.4
1,597,537 - -2.0
1,597,540 + -3.2
1,597,540 + -0.9
1,598,113 - -2.0
1,598,332 + Psest_1496 0.29 -1.8
1,598,333 - Psest_1496 0.29 -3.3
1,598,333 - Psest_1496 0.29 -2.6
1,598,760 + +0.3
1,598,760 + -0.9
1,598,760 + +0.4
1,598,760 + -0.0
1,598,761 - +0.5

Or see this region's nucleotide sequence