Experiment: Chloride 700 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prfA and hemA are separated by 16 nucleotides hemA and Sama_2568 are separated by 229 nucleotides Sama_2568 and ipk overlap by 4 nucleotides
Sama_2566: prfA - peptide chain release factor 1 (RefSeq), at 3,071,948 to 3,073,039
prfA
Sama_2567: hemA - glutamyl-tRNA reductase (RefSeq), at 3,073,056 to 3,074,306
hemA
Sama_2568: Sama_2568 - outer membrane lipoprotein LolB (RefSeq), at 3,074,536 to 3,075,159
_2568
Sama_2569: ipk - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (RefSeq), at 3,075,156 to 3,076,007
ipk
Position (kb)
3073
3074
3075 Strain fitness (log2 ratio)
-2
-1
0
1 at 3074.356 kb on - strand at 3074.383 kb on + strand at 3074.383 kb on + strand at 3074.383 kb on + strand at 3074.383 kb on + strand at 3074.383 kb on + strand at 3074.383 kb on + strand at 3074.384 kb on - strand at 3074.384 kb on - strand at 3074.384 kb on - strand at 3074.389 kb on + strand at 3074.486 kb on - strand at 3074.505 kb on + strand at 3074.506 kb on - strand at 3074.510 kb on + strand at 3074.510 kb on + strand at 3074.518 kb on + strand at 3074.518 kb on + strand at 3074.518 kb on + strand at 3074.522 kb on + strand at 3074.533 kb on + strand at 3074.533 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 700 mM remove 3,074,356 - +1.2 3,074,383 + +0.3 3,074,383 + +1.0 3,074,383 + +0.9 3,074,383 + -1.0 3,074,383 + -2.6 3,074,383 + -0.0 3,074,384 - -0.2 3,074,384 - -2.0 3,074,384 - -0.3 3,074,389 + -1.5 3,074,486 - -0.9 3,074,505 + -0.7 3,074,506 - +1.7 3,074,510 + -0.5 3,074,510 + -0.3 3,074,518 + -0.1 3,074,518 + -0.7 3,074,518 + -2.2 3,074,522 + -1.7 3,074,533 + -0.7 3,074,533 + -1.3
Or see this region's nucleotide sequence