Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1891 and atoD are separated by 106 nucleotides atoD and mvaB are separated by 81 nucleotides mvaB and SO1894 are separated by 1 nucleotides
SO1891: SO1891 - 3-oxoadipate CoA-succinyl transferase, beta subunit (NCBI ptt file), at 1,987,930 to 1,988,586
SO1891
SO1892: atoD - acetate CoA-transferase, subunit A (NCBI ptt file), at 1,988,693 to 1,989,397
atoD
SO1893: mvaB - hydroxymethylglutaryl-CoA lyase (NCBI ptt file), at 1,989,479 to 1,990,426
mvaB
SO1894: SO1894 - acetyl-CoA carboxylase, biotin carboxylase, putative (NCBI ptt file), at 1,990,428 to 1,992,512
SO1894
Position (kb)
1989
1990
1991 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1989.017 kb on + strand, within atoD at 1989.017 kb on + strand, within atoD at 1989.025 kb on - strand, within atoD at 1989.125 kb on - strand, within atoD at 1989.133 kb on + strand, within atoD at 1989.206 kb on - strand, within atoD at 1990.469 kb on + strand at 1990.553 kb on - strand at 1990.591 kb on + strand at 1990.591 kb on + strand at 1990.591 kb on + strand at 1990.599 kb on - strand at 1990.599 kb on - strand at 1990.599 kb on - strand at 1990.623 kb on + strand at 1990.631 kb on - strand at 1990.633 kb on + strand at 1990.700 kb on - strand, within SO1894 at 1990.726 kb on + strand, within SO1894 at 1990.726 kb on + strand, within SO1894 at 1990.734 kb on - strand, within SO1894 at 1990.817 kb on + strand, within SO1894 at 1990.959 kb on - strand, within SO1894 at 1990.964 kb on - strand, within SO1894 at 1990.994 kb on - strand, within SO1894 at 1991.175 kb on - strand, within SO1894 at 1991.262 kb on + strand, within SO1894 at 1991.386 kb on + strand, within SO1894
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 1,989,017 + atoD SO1892 0.46 -3.0 1,989,017 + atoD SO1892 0.46 -1.8 1,989,025 - atoD SO1892 0.47 -3.3 1,989,125 - atoD SO1892 0.61 -1.9 1,989,133 + atoD SO1892 0.62 -1.0 1,989,206 - atoD SO1892 0.73 -2.0 1,990,469 + +2.8 1,990,553 - +2.7 1,990,591 + +2.2 1,990,591 + +1.9 1,990,591 + +1.4 1,990,599 - +1.9 1,990,599 - +1.5 1,990,599 - +1.6 1,990,623 + +1.4 1,990,631 - +2.8 1,990,633 + +1.8 1,990,700 - SO1894 0.13 +1.9 1,990,726 + SO1894 0.14 +2.4 1,990,726 + SO1894 0.14 +2.3 1,990,734 - SO1894 0.15 +1.9 1,990,817 + SO1894 0.19 +1.4 1,990,959 - SO1894 0.25 +1.9 1,990,964 - SO1894 0.26 +1.9 1,990,994 - SO1894 0.27 +2.1 1,991,175 - SO1894 0.36 +1.7 1,991,262 + SO1894 0.40 +1.9 1,991,386 + SO1894 0.46 +1.0
Or see this region's nucleotide sequence