Strain Fitness in Shewanella oneidensis MR-1 around SO1368

Experiment: L-Phenylalanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpheA and pepA-2 are separated by 370 nucleotidespepA-2 and SO1369 are separated by 255 nucleotides SO1367: pheA - chorismate mutase/prephenate dehydratase (NCBI ptt file), at 1,422,953 to 1,424,968 pheA SO1368: pepA-2 - cytosol aminopeptidase (NCBI ptt file), at 1,425,339 to 1,426,847 pepA-2 SO1369: SO1369 - conserved hypothetical protein (NCBI ptt file), at 1,427,103 to 1,428,215 SO1369 Position (kb) 1425 1426 1427Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 1424.352 kb on + strand, within pheAat 1424.352 kb on + strand, within pheAat 1424.360 kb on - strand, within pheAat 1424.362 kb on + strand, within pheAat 1424.453 kb on + strand, within pheAat 1424.453 kb on + strand, within pheAat 1424.461 kb on - strand, within pheAat 1424.649 kb on + strand, within pheAat 1424.816 kb on + strandat 1424.840 kb on + strandat 1424.866 kb on + strandat 1425.023 kb on + strandat 1425.130 kb on + strandat 1425.138 kb on - strandat 1425.143 kb on + strandat 1425.370 kb on + strandat 1425.424 kb on + strandat 1425.625 kb on + strand, within pepA-2at 1425.633 kb on - strand, within pepA-2at 1425.635 kb on + strand, within pepA-2at 1425.638 kb on - strand, within pepA-2at 1425.671 kb on - strand, within pepA-2at 1425.703 kb on + strand, within pepA-2at 1425.713 kb on + strand, within pepA-2at 1425.713 kb on + strand, within pepA-2at 1425.721 kb on - strand, within pepA-2at 1425.767 kb on - strand, within pepA-2at 1425.809 kb on + strand, within pepA-2at 1425.859 kb on + strand, within pepA-2at 1425.859 kb on + strand, within pepA-2at 1425.893 kb on - strand, within pepA-2at 1425.948 kb on + strand, within pepA-2at 1425.987 kb on + strand, within pepA-2at 1426.337 kb on + strand, within pepA-2at 1426.337 kb on + strand, within pepA-2at 1426.345 kb on - strand, within pepA-2at 1426.406 kb on - strand, within pepA-2at 1426.434 kb on + strand, within pepA-2at 1426.445 kb on + strand, within pepA-2at 1426.626 kb on - strand, within pepA-2at 1426.641 kb on - strand, within pepA-2at 1426.683 kb on - strand, within pepA-2at 1426.704 kb on + strandat 1426.727 kb on - strandat 1426.756 kb on + strandat 1426.844 kb on - strandat 1426.861 kb on - strandat 1427.006 kb on + strandat 1427.006 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Phenylalanine (N)
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1,424,352 + pheA SO1367 0.69 +0.5
1,424,352 + pheA SO1367 0.69 -1.2
1,424,360 - pheA SO1367 0.70 -0.9
1,424,362 + pheA SO1367 0.70 +1.0
1,424,453 + pheA SO1367 0.74 -0.7
1,424,453 + pheA SO1367 0.74 +0.9
1,424,461 - pheA SO1367 0.75 -0.2
1,424,649 + pheA SO1367 0.84 +0.2
1,424,816 + +0.3
1,424,840 + +0.3
1,424,866 + -0.1
1,425,023 + +0.8
1,425,130 + +0.2
1,425,138 - +0.0
1,425,143 + -1.0
1,425,370 + -1.3
1,425,424 + -3.2
1,425,625 + pepA-2 SO1368 0.19 -1.7
1,425,633 - pepA-2 SO1368 0.19 -1.1
1,425,635 + pepA-2 SO1368 0.20 -4.6
1,425,638 - pepA-2 SO1368 0.20 -2.3
1,425,671 - pepA-2 SO1368 0.22 -4.0
1,425,703 + pepA-2 SO1368 0.24 -3.8
1,425,713 + pepA-2 SO1368 0.25 -1.7
1,425,713 + pepA-2 SO1368 0.25 -2.7
1,425,721 - pepA-2 SO1368 0.25 -3.8
1,425,767 - pepA-2 SO1368 0.28 -0.3
1,425,809 + pepA-2 SO1368 0.31 -4.0
1,425,859 + pepA-2 SO1368 0.34 -4.2
1,425,859 + pepA-2 SO1368 0.34 -1.9
1,425,893 - pepA-2 SO1368 0.37 -4.5
1,425,948 + pepA-2 SO1368 0.40 -5.8
1,425,987 + pepA-2 SO1368 0.43 -4.1
1,426,337 + pepA-2 SO1368 0.66 -3.3
1,426,337 + pepA-2 SO1368 0.66 -3.0
1,426,345 - pepA-2 SO1368 0.67 -2.9
1,426,406 - pepA-2 SO1368 0.71 -4.4
1,426,434 + pepA-2 SO1368 0.73 -4.3
1,426,445 + pepA-2 SO1368 0.73 -1.3
1,426,626 - pepA-2 SO1368 0.85 -2.9
1,426,641 - pepA-2 SO1368 0.86 -3.3
1,426,683 - pepA-2 SO1368 0.89 -2.3
1,426,704 + -4.8
1,426,727 - -4.8
1,426,756 + -2.9
1,426,844 - -2.4
1,426,861 - -1.1
1,427,006 + -4.1
1,427,006 + -3.9

Or see this region's nucleotide sequence