Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnE and SO3761 are separated by 147 nucleotides SO3761 and SO3762 are separated by 15 nucleotides SO3762 and SO3763 are separated by 4 nucleotides
SO3760: glnE - glutamate-ammonia-ligase adenylyltransferase (NCBI ptt file), at 3,907,349 to 3,910,321
glnE
SO3761: SO3761 - hypothetical protein (NCBI ptt file), at 3,910,469 to 3,911,458
SO3761
SO3762: SO3762 - hypothetical protein (NCBI ptt file), at 3,911,474 to 3,912,373
SO3762
SO3763: SO3763 - spermine/spermidine synthase family protein (NCBI ptt file), at 3,912,378 to 3,914,102
SO3763
Position (kb)
3910
3911
3912 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3909.560 kb on + strand, within glnE at 3909.781 kb on + strand, within glnE at 3909.789 kb on - strand, within glnE at 3909.789 kb on - strand, within glnE at 3909.843 kb on - strand, within glnE at 3909.845 kb on + strand, within glnE at 3909.913 kb on - strand, within glnE at 3909.918 kb on + strand, within glnE at 3910.011 kb on + strand, within glnE at 3910.019 kb on - strand, within glnE at 3910.029 kb on - strand at 3910.136 kb on - strand at 3910.160 kb on + strand at 3910.168 kb on - strand at 3910.213 kb on + strand at 3910.289 kb on - strand at 3910.300 kb on + strand at 3910.300 kb on + strand at 3910.300 kb on + strand at 3910.300 kb on + strand at 3910.300 kb on + strand at 3910.300 kb on + strand at 3910.300 kb on + strand at 3910.300 kb on + strand at 3910.308 kb on - strand at 3910.344 kb on + strand at 3910.380 kb on + strand at 3910.515 kb on - strand at 3910.520 kb on + strand at 3910.602 kb on - strand, within SO3761 at 3910.625 kb on + strand, within SO3761 at 3910.655 kb on + strand, within SO3761 at 3910.754 kb on + strand, within SO3761 at 3910.772 kb on - strand, within SO3761 at 3910.848 kb on + strand, within SO3761 at 3910.856 kb on - strand, within SO3761 at 3910.957 kb on + strand, within SO3761 at 3911.172 kb on - strand, within SO3761 at 3911.279 kb on + strand, within SO3761 at 3911.328 kb on + strand, within SO3761 at 3911.391 kb on + strand at 3911.399 kb on - strand at 3911.579 kb on + strand, within SO3762 at 3911.612 kb on - strand, within SO3762 at 3911.641 kb on + strand, within SO3762 at 3911.760 kb on + strand, within SO3762 at 3911.768 kb on - strand, within SO3762 at 3911.909 kb on + strand, within SO3762 at 3911.909 kb on + strand, within SO3762 at 3911.917 kb on - strand, within SO3762 at 3911.917 kb on - strand, within SO3762 at 3911.922 kb on - strand, within SO3762 at 3911.951 kb on - strand, within SO3762 at 3911.964 kb on - strand, within SO3762 at 3911.980 kb on + strand, within SO3762 at 3912.042 kb on + strand, within SO3762 at 3912.219 kb on + strand, within SO3762 at 3912.227 kb on + strand, within SO3762 at 3912.235 kb on - strand, within SO3762 at 3912.333 kb on + strand at 3912.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 3,909,560 + glnE SO3760 0.74 +0.0 3,909,781 + glnE SO3760 0.82 +0.9 3,909,789 - glnE SO3760 0.82 -1.7 3,909,789 - glnE SO3760 0.82 -1.2 3,909,843 - glnE SO3760 0.84 -0.6 3,909,845 + glnE SO3760 0.84 +0.5 3,909,913 - glnE SO3760 0.86 -1.2 3,909,918 + glnE SO3760 0.86 +0.1 3,910,011 + glnE SO3760 0.90 +0.6 3,910,019 - glnE SO3760 0.90 -1.1 3,910,029 - -1.2 3,910,136 - -1.8 3,910,160 + +2.0 3,910,168 - -1.7 3,910,213 + -0.9 3,910,289 - +1.7 3,910,300 + -0.1 3,910,300 + -2.5 3,910,300 + -0.2 3,910,300 + -0.1 3,910,300 + -0.9 3,910,300 + +1.3 3,910,300 + -1.7 3,910,300 + -2.0 3,910,308 - +0.6 3,910,344 + -0.8 3,910,380 + -0.3 3,910,515 - +1.3 3,910,520 + +1.7 3,910,602 - SO3761 0.13 +0.9 3,910,625 + SO3761 0.16 +0.5 3,910,655 + SO3761 0.19 -2.5 3,910,754 + SO3761 0.29 +0.1 3,910,772 - SO3761 0.31 +0.8 3,910,848 + SO3761 0.38 +1.0 3,910,856 - SO3761 0.39 +1.1 3,910,957 + SO3761 0.49 +1.0 3,911,172 - SO3761 0.71 +1.0 3,911,279 + SO3761 0.82 +1.3 3,911,328 + SO3761 0.87 -1.1 3,911,391 + -1.8 3,911,399 - -0.6 3,911,579 + SO3762 0.12 +1.3 3,911,612 - SO3762 0.15 -1.6 3,911,641 + SO3762 0.19 -0.1 3,911,760 + SO3762 0.32 +0.8 3,911,768 - SO3762 0.33 -0.7 3,911,909 + SO3762 0.48 -0.9 3,911,909 + SO3762 0.48 +0.1 3,911,917 - SO3762 0.49 -1.2 3,911,917 - SO3762 0.49 -0.3 3,911,922 - SO3762 0.50 +1.0 3,911,951 - SO3762 0.53 +0.6 3,911,964 - SO3762 0.54 +1.5 3,911,980 + SO3762 0.56 +1.3 3,912,042 + SO3762 0.63 +0.7 3,912,219 + SO3762 0.83 -0.2 3,912,227 + SO3762 0.84 -1.6 3,912,235 - SO3762 0.85 +1.0 3,912,333 + -2.5 3,912,398 - -1.1
Or see this region's nucleotide sequence