Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2798 and SO2799 are separated by 160 nucleotides SO2799 and SO2800 are separated by 118 nucleotides SO2800 and lpxK overlap by 11 nucleotides lpxK and msbA are separated by 0 nucleotides
SO2798: SO2798 - conserved hypothetical protein (NCBI ptt file), at 2,920,954 to 2,921,646
SO2798
SO2799: SO2799 - lipoprotein, putative (NCBI ptt file), at 2,921,807 to 2,922,271
SO2799
SO2800: SO2800 - conserved hypothetical protein (NCBI ptt file), at 2,922,390 to 2,922,569
SO2800
SO2801: lpxK - tetraacyldisaccharide 4'-kinase (NCBI ptt file), at 2,922,559 to 2,923,566
lpxK
SO2802: msbA - ABC transporter, ATP-binding protein MsbA (NCBI ptt file), at 2,923,567 to 2,925,372
msbA
Position (kb)
2922
2923
2924 Strain fitness (log2 ratio)
-1
0
1
2 at 2921.582 kb on - strand at 2921.627 kb on + strand at 2921.652 kb on + strand at 2921.834 kb on + strand at 2921.842 kb on - strand at 2921.940 kb on + strand, within SO2799 at 2921.940 kb on + strand, within SO2799 at 2921.948 kb on + strand, within SO2799 at 2921.983 kb on - strand, within SO2799 at 2922.328 kb on - strand at 2922.347 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 2,921,582 - -0.4 2,921,627 + +2.0 2,921,652 + +0.8 2,921,834 + -1.6 2,921,842 - +0.7 2,921,940 + SO2799 0.29 -1.2 2,921,940 + SO2799 0.29 -0.4 2,921,948 + SO2799 0.30 +0.0 2,921,983 - SO2799 0.38 +0.3 2,922,328 - -0.2 2,922,347 - -0.1
Or see this region's nucleotide sequence