Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1944 and phoQ are separated by 5 nucleotides phoQ and phoP overlap by 10 nucleotides phoP and SO1947 are separated by 181 nucleotides
SO1944: SO1944 - hypothetical protein (NCBI ptt file), at 2,047,075 to 2,047,770
SO1944
SO1945: phoQ - sensor protein PhoQ (NCBI ptt file), at 2,047,776 to 2,049,125
phoQ
SO1946: phoP - transcriptional regulatory protein PhoP (NCBI ptt file), at 2,049,116 to 2,049,790
phoP
SO1947: SO1947 - hypothetical protein (NCBI ptt file), at 2,049,972 to 2,050,316
SO1947
Position (kb)
2047
2048
2049
2050 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2046.855 kb on + strand at 2046.871 kb on - strand at 2047.103 kb on - strand at 2047.184 kb on + strand, within SO1944 at 2047.191 kb on - strand, within SO1944 at 2047.246 kb on - strand, within SO1944 at 2047.365 kb on - strand, within SO1944 at 2047.366 kb on - strand, within SO1944 at 2047.366 kb on - strand, within SO1944 at 2047.370 kb on + strand, within SO1944 at 2047.370 kb on + strand, within SO1944 at 2047.378 kb on - strand, within SO1944 at 2047.378 kb on - strand, within SO1944 at 2047.378 kb on - strand, within SO1944 at 2047.378 kb on - strand, within SO1944 at 2047.404 kb on + strand, within SO1944 at 2047.404 kb on + strand, within SO1944 at 2047.404 kb on + strand, within SO1944 at 2047.404 kb on + strand, within SO1944 at 2047.404 kb on + strand, within SO1944 at 2047.412 kb on - strand, within SO1944 at 2047.412 kb on - strand, within SO1944 at 2047.412 kb on - strand, within SO1944 at 2047.534 kb on + strand, within SO1944 at 2047.534 kb on + strand, within SO1944 at 2047.542 kb on - strand, within SO1944 at 2047.542 kb on - strand, within SO1944 at 2047.542 kb on - strand, within SO1944 at 2047.669 kb on - strand, within SO1944 at 2047.757 kb on - strand at 2047.871 kb on + strand at 2048.213 kb on + strand, within phoQ at 2048.215 kb on - strand, within phoQ at 2048.379 kb on - strand, within phoQ at 2048.424 kb on - strand, within phoQ at 2048.446 kb on - strand, within phoQ at 2048.452 kb on - strand, within phoQ at 2048.459 kb on - strand, within phoQ at 2048.574 kb on - strand, within phoQ at 2048.619 kb on + strand, within phoQ at 2049.336 kb on - strand, within phoP at 2049.427 kb on + strand, within phoP at 2049.437 kb on + strand, within phoP at 2049.514 kb on + strand, within phoP at 2049.559 kb on + strand, within phoP at 2049.601 kb on + strand, within phoP at 2049.709 kb on + strand, within phoP at 2049.824 kb on - strand at 2049.908 kb on - strand at 2050.057 kb on + strand, within SO1947 at 2050.057 kb on + strand, within SO1947 at 2050.067 kb on + strand, within SO1947
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 2,046,855 + +0.1 2,046,871 - +0.5 2,047,103 - +0.3 2,047,184 + SO1944 0.16 +0.8 2,047,191 - SO1944 0.17 +0.4 2,047,246 - SO1944 0.25 -2.8 2,047,365 - SO1944 0.42 -1.2 2,047,366 - SO1944 0.42 -0.6 2,047,366 - SO1944 0.42 -2.2 2,047,370 + SO1944 0.42 +0.5 2,047,370 + SO1944 0.42 -1.0 2,047,378 - SO1944 0.44 +0.4 2,047,378 - SO1944 0.44 +3.6 2,047,378 - SO1944 0.44 -2.9 2,047,378 - SO1944 0.44 +0.7 2,047,404 + SO1944 0.47 -2.5 2,047,404 + SO1944 0.47 +0.5 2,047,404 + SO1944 0.47 -0.3 2,047,404 + SO1944 0.47 +0.6 2,047,404 + SO1944 0.47 -1.0 2,047,412 - SO1944 0.48 +0.0 2,047,412 - SO1944 0.48 -1.7 2,047,412 - SO1944 0.48 +1.1 2,047,534 + SO1944 0.66 -1.9 2,047,534 + SO1944 0.66 +1.8 2,047,542 - SO1944 0.67 +0.6 2,047,542 - SO1944 0.67 -0.2 2,047,542 - SO1944 0.67 +1.4 2,047,669 - SO1944 0.85 +0.6 2,047,757 - +1.0 2,047,871 + -0.8 2,048,213 + phoQ SO1945 0.32 -3.4 2,048,215 - phoQ SO1945 0.33 -0.5 2,048,379 - phoQ SO1945 0.45 -2.5 2,048,424 - phoQ SO1945 0.48 -3.3 2,048,446 - phoQ SO1945 0.50 -0.3 2,048,452 - phoQ SO1945 0.50 -2.3 2,048,459 - phoQ SO1945 0.51 -1.5 2,048,574 - phoQ SO1945 0.59 -1.3 2,048,619 + phoQ SO1945 0.62 +0.0 2,049,336 - phoP SO1946 0.33 +0.5 2,049,427 + phoP SO1946 0.46 +1.7 2,049,437 + phoP SO1946 0.48 +0.6 2,049,514 + phoP SO1946 0.59 +0.0 2,049,559 + phoP SO1946 0.66 +2.2 2,049,601 + phoP SO1946 0.72 +2.6 2,049,709 + phoP SO1946 0.88 +1.9 2,049,824 - +0.8 2,049,908 - +1.3 2,050,057 + SO1947 0.25 -0.5 2,050,057 + SO1947 0.25 -1.1 2,050,067 + SO1947 0.28 -0.1
Or see this region's nucleotide sequence