Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutL and miaA overlap by 8 nucleotides miaA and hfq are separated by 91 nucleotides hfq and hflX are separated by 23 nucleotides
SO0601: mutL - DNA mismatch repair protein MutL (NCBI ptt file), at 626,850 to 628,745
mutL
SO0602: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI ptt file), at 628,738 to 629,664
miaA
SO0603: hfq - host factor-I protein (NCBI ptt file), at 629,756 to 630,028
hfq
SO0604: hflX - GTP-binding protein HflX (NCBI ptt file), at 630,052 to 631,359
hflX
Position (kb) 628
629
630 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9 at 627.912 kb on - strand, within mutL at 627.972 kb on + strand, within mutL at 627.972 kb on + strand, within mutL at 627.975 kb on + strand, within mutL at 627.978 kb on - strand, within mutL at 628.050 kb on + strand, within mutL at 628.070 kb on - strand, within mutL at 628.126 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.199 kb on + strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.207 kb on - strand, within mutL at 628.212 kb on - strand, within mutL at 628.529 kb on + strand, within mutL at 628.537 kb on - strand, within mutL at 628.537 kb on - strand, within mutL at 628.695 kb on + strand at 628.891 kb on + strand, within miaA at 629.010 kb on + strand, within miaA at 629.302 kb on + strand, within miaA at 629.395 kb on + strand, within miaA at 629.471 kb on + strand, within miaA at 629.942 kb on + strand, within hfq at 629.999 kb on + strand, within hfq at 630.310 kb on + strand, within hflX at 630.354 kb on + strand, within hflX at 630.359 kb on + strand, within hflX at 630.402 kb on - strand, within hflX at 630.502 kb on + strand, within hflX
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 627,912 - mutL SO0601 0.56 +1.7 627,972 + mutL SO0601 0.59 +0.0 627,972 + mutL SO0601 0.59 +2.6 627,975 + mutL SO0601 0.59 +2.0 627,978 - mutL SO0601 0.59 -0.3 628,050 + mutL SO0601 0.63 +1.3 628,070 - mutL SO0601 0.64 +0.5 628,126 + mutL SO0601 0.67 +1.3 628,199 + mutL SO0601 0.71 +1.8 628,199 + mutL SO0601 0.71 -1.0 628,199 + mutL SO0601 0.71 -0.5 628,199 + mutL SO0601 0.71 +0.5 628,199 + mutL SO0601 0.71 +0.8 628,199 + mutL SO0601 0.71 +1.8 628,199 + mutL SO0601 0.71 -1.8 628,199 + mutL SO0601 0.71 -0.9 628,207 - mutL SO0601 0.72 -0.4 628,207 - mutL SO0601 0.72 +2.3 628,207 - mutL SO0601 0.72 -0.3 628,207 - mutL SO0601 0.72 +1.3 628,207 - mutL SO0601 0.72 +2.0 628,207 - mutL SO0601 0.72 +2.7 628,207 - mutL SO0601 0.72 +2.7 628,207 - mutL SO0601 0.72 +1.4 628,207 - mutL SO0601 0.72 +0.9 628,207 - mutL SO0601 0.72 +1.4 628,207 - mutL SO0601 0.72 +2.5 628,207 - mutL SO0601 0.72 +1.3 628,207 - mutL SO0601 0.72 +2.4 628,212 - mutL SO0601 0.72 +0.3 628,529 + mutL SO0601 0.89 +2.7 628,537 - mutL SO0601 0.89 +5.3 628,537 - mutL SO0601 0.89 +3.0 628,695 + +1.0 628,891 + miaA SO0602 0.17 +1.0 629,010 + miaA SO0602 0.29 +2.4 629,302 + miaA SO0602 0.61 +1.8 629,395 + miaA SO0602 0.71 +0.1 629,471 + miaA SO0602 0.79 +1.6 629,942 + hfq SO0603 0.68 +8.8 629,999 + hfq SO0603 0.89 +6.7 630,310 + hflX SO0604 0.20 +6.0 630,354 + hflX SO0604 0.23 +5.2 630,359 + hflX SO0604 0.23 +5.4 630,402 - hflX SO0604 0.27 +2.7 630,502 + hflX SO0604 0.34 +5.5
Or see this region's nucleotide sequence