Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1679 and SO1680 are separated by 52 nucleotides SO1680 and SO1681 overlap by 1 nucleotides SO1681 and mmsB are separated by 83 nucleotides
SO1679: SO1679 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 1,766,172 to 1,767,329
SO1679
SO1680: SO1680 - enoyl-CoA hydratase/isomerase family protein (NCBI ptt file), at 1,767,382 to 1,768,155
SO1680
SO1681: SO1681 - enoyl-CoA hydratase/isomerase family protein (NCBI ptt file), at 1,768,155 to 1,769,306
SO1681
SO1682: mmsB - 3-hydroxyisobutyrate dehydrogenase (NCBI ptt file), at 1,769,390 to 1,770,292
mmsB
Position (kb)
1768
1769
1770 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1767.267 kb on - strand at 1767.350 kb on + strand at 1767.358 kb on - strand at 1767.370 kb on - strand at 1767.461 kb on + strand, within SO1680 at 1767.475 kb on - strand, within SO1680 at 1767.572 kb on + strand, within SO1680 at 1767.600 kb on + strand, within SO1680 at 1767.600 kb on + strand, within SO1680 at 1767.600 kb on + strand, within SO1680 at 1767.608 kb on - strand, within SO1680 at 1767.627 kb on - strand, within SO1680 at 1767.640 kb on + strand, within SO1680 at 1767.655 kb on - strand, within SO1680 at 1767.715 kb on - strand, within SO1680 at 1767.718 kb on + strand, within SO1680 at 1767.718 kb on + strand, within SO1680 at 1767.723 kb on + strand, within SO1680 at 1767.792 kb on + strand, within SO1680 at 1767.795 kb on - strand, within SO1680 at 1767.837 kb on + strand, within SO1680 at 1767.845 kb on - strand, within SO1680 at 1767.845 kb on - strand, within SO1680 at 1768.106 kb on - strand at 1768.128 kb on + strand at 1768.184 kb on + strand at 1768.280 kb on - strand, within SO1681 at 1768.526 kb on + strand, within SO1681 at 1768.526 kb on + strand, within SO1681 at 1768.526 kb on + strand, within SO1681 at 1768.526 kb on + strand, within SO1681 at 1768.650 kb on + strand, within SO1681 at 1768.717 kb on + strand, within SO1681 at 1768.752 kb on - strand, within SO1681 at 1768.880 kb on - strand, within SO1681 at 1768.921 kb on + strand, within SO1681 at 1769.040 kb on + strand, within SO1681 at 1769.040 kb on + strand, within SO1681 at 1769.080 kb on - strand, within SO1681 at 1769.090 kb on + strand, within SO1681 at 1769.240 kb on - strand at 1769.242 kb on + strand at 1769.250 kb on - strand at 1769.276 kb on - strand at 1769.309 kb on - strand at 1769.340 kb on - strand at 1769.375 kb on - strand at 1769.392 kb on + strand at 1769.402 kb on - strand at 1769.438 kb on - strand at 1769.707 kb on + strand, within mmsB at 1769.726 kb on + strand, within mmsB at 1769.783 kb on + strand, within mmsB at 1769.835 kb on - strand, within mmsB at 1769.844 kb on - strand, within mmsB at 1769.895 kb on - strand, within mmsB at 1769.960 kb on - strand, within mmsB at 1769.981 kb on - strand, within mmsB at 1770.060 kb on - strand, within mmsB at 1770.106 kb on - strand, within mmsB
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,767,267 - -1.3 1,767,350 + +0.4 1,767,358 - -1.4 1,767,370 - +0.3 1,767,461 + SO1680 0.10 -1.2 1,767,475 - SO1680 0.12 -1.0 1,767,572 + SO1680 0.25 -1.6 1,767,600 + SO1680 0.28 +1.0 1,767,600 + SO1680 0.28 -1.1 1,767,600 + SO1680 0.28 -1.0 1,767,608 - SO1680 0.29 -1.5 1,767,627 - SO1680 0.32 -1.8 1,767,640 + SO1680 0.33 -0.7 1,767,655 - SO1680 0.35 -0.9 1,767,715 - SO1680 0.43 -1.5 1,767,718 + SO1680 0.43 +0.0 1,767,718 + SO1680 0.43 -0.7 1,767,723 + SO1680 0.44 -0.3 1,767,792 + SO1680 0.53 -1.1 1,767,795 - SO1680 0.53 -1.2 1,767,837 + SO1680 0.59 -0.7 1,767,845 - SO1680 0.60 +0.6 1,767,845 - SO1680 0.60 -0.9 1,768,106 - -0.7 1,768,128 + -0.1 1,768,184 + -2.7 1,768,280 - SO1681 0.11 -2.5 1,768,526 + SO1681 0.32 -2.3 1,768,526 + SO1681 0.32 -1.8 1,768,526 + SO1681 0.32 -0.7 1,768,526 + SO1681 0.32 -5.3 1,768,650 + SO1681 0.43 -3.4 1,768,717 + SO1681 0.49 -2.8 1,768,752 - SO1681 0.52 -2.6 1,768,880 - SO1681 0.63 -2.5 1,768,921 + SO1681 0.66 -1.1 1,769,040 + SO1681 0.77 -1.4 1,769,040 + SO1681 0.77 -3.1 1,769,080 - SO1681 0.80 -2.7 1,769,090 + SO1681 0.81 -1.8 1,769,240 - -0.8 1,769,242 + +0.0 1,769,250 - -2.5 1,769,276 - -0.9 1,769,309 - +0.1 1,769,340 - -1.1 1,769,375 - -0.2 1,769,392 + +0.5 1,769,402 - -0.1 1,769,438 - -0.9 1,769,707 + mmsB SO1682 0.35 -0.6 1,769,726 + mmsB SO1682 0.37 -0.0 1,769,783 + mmsB SO1682 0.44 +0.2 1,769,835 - mmsB SO1682 0.49 +0.0 1,769,844 - mmsB SO1682 0.50 -0.4 1,769,895 - mmsB SO1682 0.56 -0.7 1,769,960 - mmsB SO1682 0.63 +0.0 1,769,981 - mmsB SO1682 0.65 -0.6 1,770,060 - mmsB SO1682 0.74 +0.1 1,770,106 - mmsB SO1682 0.79 -0.6
Or see this region's nucleotide sequence