Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phhA and SO1667 are separated by 84 nucleotides SO1667 and tyrR are separated by 260 nucleotides tyrR and SO1670 are separated by 341 nucleotides
SO1666: phhA - Phenylalanine 4-monooxygenase (EC 1.14.16.1) (from data) , at 1,753,458 to 1,754,273
phhA
SO1667: SO1667 - pterin-4-alpha-carbinolamine dehydratase (NCBI ptt file), at 1,754,358 to 1,754,696
SO1667
SO1669: tyrR - transcriptional regulatory protein TyrR (NCBI ptt file), at 1,754,957 to 1,756,495
tyrR
SO1670: SO1670 - fumarylacetoacetate hydrolase (EC 3.7.1.2) (from data) , at 1,756,837 to 1,757,823
SO1670
Position (kb)
1754
1755
1756
1757 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1753.958 kb on + strand, within phhA at 1753.984 kb on + strand, within phhA at 1753.992 kb on - strand, within phhA at 1753.993 kb on - strand, within phhA at 1754.105 kb on + strand, within phhA at 1754.111 kb on + strand, within phhA at 1754.113 kb on - strand, within phhA at 1754.113 kb on - strand, within phhA at 1754.201 kb on - strand at 1754.214 kb on - strand at 1754.216 kb on - strand at 1754.308 kb on + strand at 1754.317 kb on + strand at 1754.339 kb on - strand at 1754.362 kb on - strand at 1754.373 kb on - strand at 1754.388 kb on - strand at 1754.412 kb on - strand, within SO1667 at 1754.453 kb on + strand, within SO1667 at 1754.632 kb on + strand, within SO1667 at 1754.634 kb on + strand, within SO1667 at 1754.634 kb on + strand, within SO1667 at 1754.774 kb on - strand at 1754.779 kb on - strand at 1755.012 kb on - strand at 1755.168 kb on + strand, within tyrR at 1755.211 kb on + strand, within tyrR at 1755.297 kb on - strand, within tyrR at 1755.314 kb on - strand, within tyrR at 1755.376 kb on + strand, within tyrR at 1755.589 kb on + strand, within tyrR at 1755.669 kb on + strand, within tyrR at 1755.731 kb on + strand, within tyrR at 1755.754 kb on - strand, within tyrR at 1755.829 kb on + strand, within tyrR at 1755.835 kb on + strand, within tyrR at 1755.837 kb on - strand, within tyrR at 1755.837 kb on - strand, within tyrR at 1755.840 kb on + strand, within tyrR at 1755.894 kb on - strand, within tyrR at 1756.231 kb on + strand, within tyrR at 1756.239 kb on - strand, within tyrR at 1756.239 kb on - strand, within tyrR at 1756.307 kb on + strand, within tyrR at 1756.315 kb on - strand, within tyrR at 1756.337 kb on - strand, within tyrR at 1756.454 kb on - strand at 1756.454 kb on - strand at 1756.666 kb on - strand at 1756.701 kb on + strand at 1756.701 kb on + strand at 1756.701 kb on + strand at 1756.701 kb on + strand at 1756.701 kb on + strand at 1756.701 kb on + strand at 1756.709 kb on - strand at 1756.709 kb on - strand at 1756.709 kb on - strand at 1756.709 kb on - strand at 1756.709 kb on - strand at 1756.709 kb on - strand at 1756.709 kb on - strand at 1756.790 kb on - strand at 1756.944 kb on + strand, within SO1670 at 1756.966 kb on - strand, within SO1670 at 1756.968 kb on + strand, within SO1670 at 1757.026 kb on + strand, within SO1670 at 1757.162 kb on - strand, within SO1670 at 1757.312 kb on + strand, within SO1670
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,753,958 + phhA SO1666 0.61 +0.2 1,753,984 + phhA SO1666 0.64 -0.7 1,753,992 - phhA SO1666 0.65 +0.4 1,753,993 - phhA SO1666 0.66 -0.1 1,754,105 + phhA SO1666 0.79 +0.3 1,754,111 + phhA SO1666 0.80 +0.4 1,754,113 - phhA SO1666 0.80 -0.1 1,754,113 - phhA SO1666 0.80 -0.2 1,754,201 - +0.3 1,754,214 - -0.1 1,754,216 - -0.0 1,754,308 + +0.7 1,754,317 + +0.1 1,754,339 - +1.4 1,754,362 - -0.8 1,754,373 - -0.8 1,754,388 - -0.8 1,754,412 - SO1667 0.16 -0.9 1,754,453 + SO1667 0.28 +0.5 1,754,632 + SO1667 0.81 -0.2 1,754,634 + SO1667 0.81 +0.6 1,754,634 + SO1667 0.81 +0.3 1,754,774 - -0.2 1,754,779 - +0.1 1,755,012 - -0.4 1,755,168 + tyrR SO1669 0.14 -2.0 1,755,211 + tyrR SO1669 0.17 -2.6 1,755,297 - tyrR SO1669 0.22 -1.1 1,755,314 - tyrR SO1669 0.23 -3.5 1,755,376 + tyrR SO1669 0.27 -3.5 1,755,589 + tyrR SO1669 0.41 -0.6 1,755,669 + tyrR SO1669 0.46 -2.5 1,755,731 + tyrR SO1669 0.50 -1.8 1,755,754 - tyrR SO1669 0.52 -2.6 1,755,829 + tyrR SO1669 0.57 -3.3 1,755,835 + tyrR SO1669 0.57 -3.4 1,755,837 - tyrR SO1669 0.57 -1.4 1,755,837 - tyrR SO1669 0.57 -4.1 1,755,840 + tyrR SO1669 0.57 -1.8 1,755,894 - tyrR SO1669 0.61 -2.4 1,756,231 + tyrR SO1669 0.83 -4.1 1,756,239 - tyrR SO1669 0.83 -1.9 1,756,239 - tyrR SO1669 0.83 -3.2 1,756,307 + tyrR SO1669 0.88 -3.5 1,756,315 - tyrR SO1669 0.88 -3.4 1,756,337 - tyrR SO1669 0.90 -2.0 1,756,454 - -2.5 1,756,454 - -3.2 1,756,666 - -0.8 1,756,701 + +0.3 1,756,701 + -0.5 1,756,701 + -0.4 1,756,701 + -0.2 1,756,701 + +0.2 1,756,701 + -0.2 1,756,709 - -0.2 1,756,709 - -0.0 1,756,709 - -0.2 1,756,709 - +0.4 1,756,709 - -0.1 1,756,709 - -0.1 1,756,709 - -0.6 1,756,790 - -0.1 1,756,944 + SO1670 0.11 -0.0 1,756,966 - SO1670 0.13 -1.4 1,756,968 + SO1670 0.13 +0.1 1,757,026 + SO1670 0.19 -0.9 1,757,162 - SO1670 0.33 +0.3 1,757,312 + SO1670 0.48 -0.0
Or see this region's nucleotide sequence