Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0861 and serA are separated by 134 nucleotides serA and SO0863 are separated by 157 nucleotides
SO0861: SO0861 - conserved hypothetical protein (NCBI ptt file), at 889,184 to 890,140
SO0861
SO0862: serA - D-3-phosphoglycerate dehydrogenase (NCBI ptt file), at 890,275 to 891,504
serA
SO0863: SO0863 - conserved hypothetical protein (NCBI ptt file), at 891,662 to 892,267
SO0863
Position (kb) 890
891
892 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 890.181 kb on - strand at 890.329 kb on + strand at 890.372 kb on - strand at 890.419 kb on - strand, within serA at 890.424 kb on + strand, within serA at 890.426 kb on + strand, within serA at 890.484 kb on + strand, within serA at 890.517 kb on + strand, within serA at 890.517 kb on + strand, within serA at 890.525 kb on - strand, within serA at 890.527 kb on + strand, within serA at 890.636 kb on - strand, within serA at 890.756 kb on - strand, within serA at 890.783 kb on - strand, within serA at 890.933 kb on + strand, within serA at 890.933 kb on + strand, within serA at 890.933 kb on + strand, within serA at 890.941 kb on - strand, within serA at 890.941 kb on - strand, within serA at 890.982 kb on + strand, within serA at 890.990 kb on - strand, within serA at 890.990 kb on - strand, within serA at 891.080 kb on + strand, within serA at 891.118 kb on - strand, within serA at 891.199 kb on + strand, within serA at 891.254 kb on + strand, within serA at 891.298 kb on - strand, within serA at 891.361 kb on + strand, within serA at 891.369 kb on - strand, within serA at 891.375 kb on - strand, within serA at 891.558 kb on + strand at 891.600 kb on + strand at 891.634 kb on + strand at 891.701 kb on + strand at 891.741 kb on - strand, within SO0863 at 891.749 kb on + strand, within SO0863 at 891.757 kb on - strand, within SO0863 at 891.872 kb on + strand, within SO0863 at 891.880 kb on - strand, within SO0863 at 891.880 kb on - strand, within SO0863 at 891.880 kb on - strand, within SO0863 at 891.916 kb on + strand, within SO0863 at 892.036 kb on - strand, within SO0863 at 892.126 kb on - strand, within SO0863 at 892.135 kb on + strand, within SO0863 at 892.144 kb on + strand, within SO0863 at 892.252 kb on + strand at 892.252 kb on + strand at 892.260 kb on - strand at 892.260 kb on - strand at 892.260 kb on - strand at 892.267 kb on - strand at 892.274 kb on + strand at 892.296 kb on + strand at 892.397 kb on + strand at 892.397 kb on + strand at 892.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 890,181 - -0.8 890,329 + -4.1 890,372 - -1.8 890,419 - serA SO0862 0.12 -5.5 890,424 + serA SO0862 0.12 -4.2 890,426 + serA SO0862 0.12 -3.4 890,484 + serA SO0862 0.17 -3.2 890,517 + serA SO0862 0.20 -5.7 890,517 + serA SO0862 0.20 -4.9 890,525 - serA SO0862 0.20 -4.9 890,527 + serA SO0862 0.20 -4.0 890,636 - serA SO0862 0.29 -3.7 890,756 - serA SO0862 0.39 -6.1 890,783 - serA SO0862 0.41 -3.9 890,933 + serA SO0862 0.53 -5.2 890,933 + serA SO0862 0.53 -2.7 890,933 + serA SO0862 0.53 -5.8 890,941 - serA SO0862 0.54 -2.9 890,941 - serA SO0862 0.54 -5.7 890,982 + serA SO0862 0.57 -6.0 890,990 - serA SO0862 0.58 -3.4 890,990 - serA SO0862 0.58 -3.6 891,080 + serA SO0862 0.65 -4.8 891,118 - serA SO0862 0.69 -3.3 891,199 + serA SO0862 0.75 -3.8 891,254 + serA SO0862 0.80 -2.3 891,298 - serA SO0862 0.83 -3.7 891,361 + serA SO0862 0.88 -3.1 891,369 - serA SO0862 0.89 -6.6 891,375 - serA SO0862 0.89 -2.7 891,558 + -3.3 891,600 + -0.5 891,634 + +1.2 891,701 + -0.1 891,741 - SO0863 0.13 -0.3 891,749 + SO0863 0.14 -0.0 891,757 - SO0863 0.16 +1.2 891,872 + SO0863 0.35 -0.6 891,880 - SO0863 0.36 +0.1 891,880 - SO0863 0.36 -0.8 891,880 - SO0863 0.36 -0.3 891,916 + SO0863 0.42 +1.5 892,036 - SO0863 0.62 +0.1 892,126 - SO0863 0.77 +0.1 892,135 + SO0863 0.78 -0.6 892,144 + SO0863 0.80 +0.2 892,252 + +0.1 892,252 + -0.3 892,260 - +0.5 892,260 - +0.4 892,260 - +0.1 892,267 - +0.1 892,274 + +0.8 892,296 + -0.1 892,397 + +0.3 892,397 + +0.4 892,404 - -0.2
Or see this region's nucleotide sequence