Strain Fitness in Shewanella oneidensis MR-1 around SO0445

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO0444 and SO0445 are separated by 105 nucleotidesSO0445 and SO0447 are separated by 332 nucleotidesSO0447 and SO0448 are separated by 3 nucleotidesSO0448 and SO0449 are separated by 6 nucleotides SO0444: SO0444 - hypothetical protein (NCBI ptt file), at 472,249 to 473,724 SO0444 SO0445: SO0445 - hflC protein, putative (NCBI ptt file), at 473,830 to 474,720 SO0445 SO0447: SO0447 - hypothetical protein (NCBI ptt file), at 475,053 to 475,307 SO0447 SO0448: SO0448 - hypothetical protein (NCBI ptt file), at 475,311 to 475,601 SO0448 SO0449: SO0449 - conserved hypothetical protein (NCBI ptt file), at 475,608 to 477,227 SO0449 Position (kb) 473 474 475Strain fitness (log2 ratio) -1 0 1at 472.963 kb on + strand, within SO0444at 472.964 kb on + strand, within SO0444at 472.965 kb on + strand, within SO0444at 472.983 kb on - strand, within SO0444at 473.001 kb on - strand, within SO0444at 473.088 kb on + strand, within SO0444at 473.202 kb on - strand, within SO0444at 473.207 kb on + strand, within SO0444at 473.346 kb on - strand, within SO0444at 473.348 kb on - strand, within SO0444at 473.418 kb on - strand, within SO0444at 473.490 kb on + strand, within SO0444at 473.490 kb on + strand, within SO0444at 473.527 kb on - strand, within SO0444at 473.570 kb on + strand, within SO0444at 473.578 kb on - strandat 473.602 kb on + strandat 473.653 kb on - strandat 473.653 kb on - strandat 473.793 kb on + strandat 473.923 kb on - strand, within SO0445at 474.047 kb on + strand, within SO0445at 474.123 kb on - strand, within SO0445at 474.195 kb on + strand, within SO0445at 474.195 kb on + strand, within SO0445at 474.203 kb on - strand, within SO0445at 474.203 kb on - strand, within SO0445at 474.203 kb on - strand, within SO0445at 474.203 kb on - strand, within SO0445at 474.213 kb on - strand, within SO0445at 474.217 kb on + strand, within SO0445at 474.268 kb on - strand, within SO0445at 474.279 kb on - strand, within SO0445at 474.427 kb on - strand, within SO0445at 474.488 kb on + strand, within SO0445at 474.488 kb on - strand, within SO0445at 474.488 kb on - strand, within SO0445at 474.606 kb on + strand, within SO0445at 474.614 kb on - strand, within SO0445at 474.665 kb on + strandat 474.734 kb on - strandat 474.750 kb on - strandat 474.905 kb on - strandat 474.905 kb on - strandat 475.083 kb on + strand, within SO0447at 475.141 kb on + strand, within SO0447at 475.177 kb on + strand, within SO0447at 475.177 kb on + strand, within SO0447at 475.179 kb on - strand, within SO0447at 475.182 kb on + strand, within SO0447at 475.185 kb on - strand, within SO0447at 475.362 kb on - strand, within SO0448at 475.424 kb on + strand, within SO0448at 475.431 kb on - strand, within SO0448at 475.478 kb on + strand, within SO0448at 475.598 kb on - strandat 475.635 kb on - strandat 475.635 kb on - strandat 475.652 kb on + strandat 475.718 kb on + strandat 475.718 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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472,963 + SO0444 0.48 -0.4
472,964 + SO0444 0.48 -0.8
472,965 + SO0444 0.49 -0.0
472,983 - SO0444 0.50 +0.6
473,001 - SO0444 0.51 +0.9
473,088 + SO0444 0.57 +1.6
473,202 - SO0444 0.65 -0.3
473,207 + SO0444 0.65 -0.0
473,346 - SO0444 0.74 +0.3
473,348 - SO0444 0.74 -0.3
473,418 - SO0444 0.79 -0.1
473,490 + SO0444 0.84 -0.2
473,490 + SO0444 0.84 +0.3
473,527 - SO0444 0.87 +0.8
473,570 + SO0444 0.89 +0.2
473,578 - +0.0
473,602 + +0.2
473,653 - -0.1
473,653 - -0.1
473,793 + +0.6
473,923 - SO0445 0.10 -0.7
474,047 + SO0445 0.24 -0.5
474,123 - SO0445 0.33 +0.2
474,195 + SO0445 0.41 +0.4
474,195 + SO0445 0.41 +0.0
474,203 - SO0445 0.42 +0.5
474,203 - SO0445 0.42 -0.4
474,203 - SO0445 0.42 -0.4
474,203 - SO0445 0.42 +0.3
474,213 - SO0445 0.43 -0.2
474,217 + SO0445 0.43 +1.1
474,268 - SO0445 0.49 +1.6
474,279 - SO0445 0.50 -1.0
474,427 - SO0445 0.67 +0.2
474,488 + SO0445 0.74 +0.5
474,488 - SO0445 0.74 +0.9
474,488 - SO0445 0.74 -0.1
474,606 + SO0445 0.87 +0.1
474,614 - SO0445 0.88 -0.1
474,665 + -0.1
474,734 - +0.3
474,750 - +0.1
474,905 - +0.6
474,905 - -1.7
475,083 + SO0447 0.12 +0.4
475,141 + SO0447 0.35 -0.2
475,177 + SO0447 0.49 -1.2
475,177 + SO0447 0.49 -0.7
475,179 - SO0447 0.49 -0.0
475,182 + SO0447 0.51 +0.2
475,185 - SO0447 0.52 -0.7
475,362 - SO0448 0.18 +0.0
475,424 + SO0448 0.39 +0.0
475,431 - SO0448 0.41 -0.2
475,478 + SO0448 0.57 +0.9
475,598 - +0.2
475,635 - -1.0
475,635 - -0.2
475,652 + -0.2
475,718 + +1.2
475,718 + -0.4

Or see this region's nucleotide sequence