Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prpC and prpB are separated by 234 nucleotides prpB and SO0346 are separated by 63 nucleotides
SO0344: prpC - 2-methylcitrate synthase (EC 2.3.3.5) <small>(from data)</small>, at 350,228 to 351,355
prpC
SO0345: prpB - methylisocitrate lyase (NCBI ptt file), at 351,590 to 352,468
prpB
SO0346: SO0346 - transcriptional regulator. GntR family (NCBI ptt file), at 352,532 to 353,248
SO0346
Position (kb) 351
352
353 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 350.615 kb on - strand, within prpC at 350.623 kb on + strand, within prpC at 350.695 kb on - strand, within prpC at 350.777 kb on + strand, within prpC at 350.792 kb on - strand, within prpC at 350.842 kb on + strand, within prpC at 350.842 kb on + strand, within prpC at 350.850 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.045 kb on - strand, within prpC at 351.115 kb on + strand, within prpC at 351.163 kb on + strand, within prpC at 351.199 kb on - strand, within prpC at 351.277 kb on - strand at 351.287 kb on + strand at 351.288 kb on - strand at 351.295 kb on - strand at 351.317 kb on + strand at 351.455 kb on + strand at 351.474 kb on - strand at 351.575 kb on - strand at 351.587 kb on - strand at 351.668 kb on + strand at 351.668 kb on + strand at 351.668 kb on + strand at 351.668 kb on + strand at 351.676 kb on - strand at 351.680 kb on + strand, within prpB at 351.680 kb on + strand, within prpB at 351.680 kb on + strand, within prpB at 351.680 kb on + strand, within prpB at 351.680 kb on + strand, within prpB at 351.680 kb on + strand, within prpB at 351.713 kb on - strand, within prpB at 351.776 kb on - strand, within prpB at 351.805 kb on - strand, within prpB at 351.866 kb on + strand, within prpB at 351.869 kb on - strand, within prpB at 351.910 kb on - strand, within prpB at 351.978 kb on - strand, within prpB at 352.131 kb on + strand, within prpB at 352.343 kb on + strand, within prpB at 352.517 kb on + strand at 352.661 kb on - strand, within SO0346 at 352.678 kb on - strand, within SO0346 at 352.679 kb on - strand, within SO0346 at 352.723 kb on - strand, within SO0346 at 352.723 kb on - strand, within SO0346 at 352.810 kb on - strand, within SO0346 at 352.870 kb on + strand, within SO0346 at 352.964 kb on - strand, within SO0346 at 352.968 kb on + strand, within SO0346 at 353.059 kb on + strand, within SO0346 at 353.165 kb on - strand, within SO0346 at 353.197 kb on - strand at 353.198 kb on + strand at 353.240 kb on - strand at 353.260 kb on - strand at 353.283 kb on - strand at 353.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 350,615 - prpC SO0344 0.34 -0.7 350,623 + prpC SO0344 0.35 -0.3 350,695 - prpC SO0344 0.41 -1.6 350,777 + prpC SO0344 0.49 -1.4 350,792 - prpC SO0344 0.50 +0.6 350,842 + prpC SO0344 0.54 -1.5 350,842 + prpC SO0344 0.54 -0.2 350,850 - prpC SO0344 0.55 -0.2 351,029 - prpC SO0344 0.71 -2.5 351,029 - prpC SO0344 0.71 -1.8 351,029 - prpC SO0344 0.71 -0.6 351,029 - prpC SO0344 0.71 +0.2 351,045 - prpC SO0344 0.72 -0.6 351,115 + prpC SO0344 0.79 -0.9 351,163 + prpC SO0344 0.83 -0.3 351,199 - prpC SO0344 0.86 +0.0 351,277 - -0.9 351,287 + +0.5 351,288 - -1.2 351,295 - -1.6 351,317 + -0.9 351,455 + +0.2 351,474 - -0.0 351,575 - -0.3 351,587 - -1.8 351,668 + -0.5 351,668 + -0.3 351,668 + -1.2 351,668 + -2.0 351,676 - -1.4 351,680 + prpB SO0345 0.10 -1.6 351,680 + prpB SO0345 0.10 -1.0 351,680 + prpB SO0345 0.10 -1.4 351,680 + prpB SO0345 0.10 -0.1 351,680 + prpB SO0345 0.10 -0.3 351,680 + prpB SO0345 0.10 -0.6 351,713 - prpB SO0345 0.14 -1.6 351,776 - prpB SO0345 0.21 -3.8 351,805 - prpB SO0345 0.24 -2.9 351,866 + prpB SO0345 0.31 -0.6 351,869 - prpB SO0345 0.32 -2.5 351,910 - prpB SO0345 0.36 -2.0 351,978 - prpB SO0345 0.44 -2.8 352,131 + prpB SO0345 0.62 -1.5 352,343 + prpB SO0345 0.86 -0.8 352,517 + +0.0 352,661 - SO0346 0.18 -0.7 352,678 - SO0346 0.20 -0.0 352,679 - SO0346 0.21 -0.0 352,723 - SO0346 0.27 +0.5 352,723 - SO0346 0.27 +2.1 352,810 - SO0346 0.39 -0.7 352,870 + SO0346 0.47 -0.0 352,964 - SO0346 0.60 -1.0 352,968 + SO0346 0.61 -0.9 353,059 + SO0346 0.74 -1.2 353,165 - SO0346 0.88 -1.2 353,197 - -0.1 353,198 + -0.2 353,240 - -0.1 353,260 - -1.4 353,283 - -2.2 353,334 - -0.5
Or see this region's nucleotide sequence