Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argG and argH are separated by 91 nucleotides argH and mrcA are separated by 74 nucleotides
SO0278: argG - argininosuccinate synthase (NCBI ptt file), at 283,296 to 284,519
argG
SO0279: argH - argininosuccinate lyase (NCBI ptt file), at 284,611 to 285,978
argH
SO0280: mrcA - penicillin-binding protein 1A (NCBI ptt file), at 286,053 to 288,572
mrcA
Position (kb) 284
285
286 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 283.623 kb on - strand, within argG at 283.636 kb on - strand, within argG at 283.636 kb on - strand, within argG at 283.662 kb on - strand, within argG at 283.678 kb on - strand, within argG at 283.722 kb on - strand, within argG at 283.730 kb on - strand, within argG at 283.770 kb on + strand, within argG at 283.770 kb on + strand, within argG at 283.770 kb on + strand, within argG at 283.770 kb on + strand, within argG at 283.770 kb on + strand, within argG at 283.773 kb on - strand, within argG at 283.778 kb on - strand, within argG at 283.798 kb on + strand, within argG at 283.841 kb on + strand, within argG at 283.900 kb on - strand, within argG at 283.971 kb on + strand, within argG at 284.034 kb on - strand, within argG at 284.048 kb on + strand, within argG at 284.080 kb on + strand, within argG at 284.165 kb on - strand, within argG at 284.184 kb on + strand, within argG at 284.226 kb on + strand, within argG at 284.250 kb on + strand, within argG at 284.256 kb on + strand, within argG at 284.264 kb on - strand, within argG at 284.354 kb on + strand, within argG at 284.376 kb on + strand, within argG at 284.379 kb on + strand, within argG at 284.407 kb on - strand at 284.463 kb on + strand at 284.516 kb on + strand at 284.524 kb on - strand at 284.581 kb on - strand at 284.622 kb on - strand at 284.622 kb on - strand at 284.717 kb on + strand at 284.868 kb on - strand, within argH at 285.133 kb on - strand, within argH at 285.154 kb on - strand, within argH at 285.236 kb on + strand, within argH at 285.244 kb on - strand, within argH at 285.261 kb on - strand, within argH at 285.283 kb on + strand, within argH at 285.319 kb on + strand, within argH at 285.322 kb on - strand, within argH at 285.324 kb on + strand, within argH at 285.342 kb on - strand, within argH at 285.403 kb on + strand, within argH at 285.403 kb on + strand, within argH at 285.403 kb on + strand, within argH at 285.403 kb on + strand, within argH at 285.505 kb on + strand, within argH at 285.587 kb on - strand, within argH at 285.623 kb on + strand, within argH at 285.669 kb on + strand, within argH at 285.669 kb on + strand, within argH at 285.696 kb on + strand, within argH at 285.735 kb on + strand, within argH at 285.784 kb on - strand, within argH at 285.821 kb on + strand, within argH at 285.837 kb on + strand, within argH at 285.846 kb on - strand at 285.908 kb on + strand at 285.939 kb on - strand at 286.059 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 283,623 - argG SO0278 0.27 -2.7 283,636 - argG SO0278 0.28 -5.2 283,636 - argG SO0278 0.28 -3.0 283,662 - argG SO0278 0.30 -4.1 283,678 - argG SO0278 0.31 -3.9 283,722 - argG SO0278 0.35 -0.5 283,730 - argG SO0278 0.35 -4.6 283,770 + argG SO0278 0.39 -4.6 283,770 + argG SO0278 0.39 -4.8 283,770 + argG SO0278 0.39 -2.4 283,770 + argG SO0278 0.39 -1.0 283,770 + argG SO0278 0.39 -2.8 283,773 - argG SO0278 0.39 -4.3 283,778 - argG SO0278 0.39 -2.6 283,798 + argG SO0278 0.41 -1.8 283,841 + argG SO0278 0.45 -3.1 283,900 - argG SO0278 0.49 -4.1 283,971 + argG SO0278 0.55 -4.2 284,034 - argG SO0278 0.60 -2.6 284,048 + argG SO0278 0.61 -3.8 284,080 + argG SO0278 0.64 -4.2 284,165 - argG SO0278 0.71 -4.4 284,184 + argG SO0278 0.73 -3.1 284,226 + argG SO0278 0.76 -1.8 284,250 + argG SO0278 0.78 -3.6 284,256 + argG SO0278 0.78 -3.7 284,264 - argG SO0278 0.79 -4.8 284,354 + argG SO0278 0.86 -3.5 284,376 + argG SO0278 0.88 -5.1 284,379 + argG SO0278 0.88 -6.1 284,407 - -4.8 284,463 + -4.5 284,516 + +0.5 284,524 - -4.0 284,581 - -1.2 284,622 - -1.7 284,622 - -2.7 284,717 + -5.5 284,868 - argH SO0279 0.19 +0.2 285,133 - argH SO0279 0.38 -4.3 285,154 - argH SO0279 0.40 -2.7 285,236 + argH SO0279 0.46 -6.1 285,244 - argH SO0279 0.46 -3.5 285,261 - argH SO0279 0.48 -4.1 285,283 + argH SO0279 0.49 -3.1 285,319 + argH SO0279 0.52 -1.2 285,322 - argH SO0279 0.52 -5.2 285,324 + argH SO0279 0.52 -4.8 285,342 - argH SO0279 0.53 -4.5 285,403 + argH SO0279 0.58 -2.9 285,403 + argH SO0279 0.58 -1.9 285,403 + argH SO0279 0.58 -5.1 285,403 + argH SO0279 0.58 -4.9 285,505 + argH SO0279 0.65 -3.8 285,587 - argH SO0279 0.71 -1.0 285,623 + argH SO0279 0.74 -4.4 285,669 + argH SO0279 0.77 -5.7 285,669 + argH SO0279 0.77 -5.5 285,696 + argH SO0279 0.79 -4.2 285,735 + argH SO0279 0.82 -3.4 285,784 - argH SO0279 0.86 -2.5 285,821 + argH SO0279 0.88 -2.8 285,837 + argH SO0279 0.90 -3.7 285,846 - -5.8 285,908 + -4.6 285,939 - -1.0 286,059 + +0.6
Or see this region's nucleotide sequence