Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argC and argB are separated by 31 nucleotides argB and argF are separated by 12 nucleotides argF and argG are separated by 59 nucleotides
SO0275: argC - N-acetyl-gamma-glutamyl-phosphate reductase (NCBI ptt file), at 280,524 to 281,504
argC
SO0276: argB - acetylglutamate kinase (NCBI ptt file), at 281,536 to 282,318
argB
SO0277: argF - ornithine carbamoyltransferase (NCBI ptt file), at 282,331 to 283,236
argF
SO0278: argG - argininosuccinate synthase (NCBI ptt file), at 283,296 to 284,519
argG
Position (kb) 281
282
283 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 280.553 kb on + strand at 280.571 kb on + strand at 280.578 kb on + strand at 280.579 kb on - strand at 280.579 kb on - strand at 280.586 kb on - strand at 280.586 kb on - strand at 280.615 kb on - strand at 280.623 kb on - strand, within argC at 280.662 kb on + strand, within argC at 280.670 kb on - strand, within argC at 280.670 kb on - strand, within argC at 280.674 kb on + strand, within argC at 280.674 kb on + strand, within argC at 280.674 kb on + strand, within argC at 280.674 kb on + strand, within argC at 280.674 kb on + strand, within argC at 280.682 kb on - strand, within argC at 280.689 kb on + strand at 280.697 kb on - strand, within argC at 280.704 kb on - strand, within argC at 280.755 kb on - strand, within argC at 280.757 kb on + strand, within argC at 280.820 kb on - strand, within argC at 280.837 kb on + strand, within argC at 280.882 kb on - strand, within argC at 280.977 kb on + strand, within argC at 281.045 kb on - strand, within argC at 281.047 kb on + strand, within argC at 281.047 kb on + strand, within argC at 281.055 kb on - strand, within argC at 281.067 kb on - strand, within argC at 281.080 kb on - strand, within argC at 281.125 kb on + strand, within argC at 281.125 kb on + strand, within argC at 281.133 kb on - strand, within argC at 281.133 kb on - strand, within argC at 281.142 kb on + strand, within argC at 281.250 kb on - strand, within argC at 281.255 kb on + strand, within argC at 281.255 kb on + strand, within argC at 281.309 kb on - strand, within argC at 281.359 kb on + strand, within argC at 281.421 kb on + strand at 281.469 kb on + strand at 281.732 kb on - strand, within argB at 281.880 kb on + strand, within argB at 281.880 kb on + strand, within argB at 282.002 kb on + strand, within argB at 282.010 kb on - strand, within argB at 282.103 kb on + strand, within argB at 282.133 kb on + strand, within argB at 282.214 kb on + strand, within argB at 282.455 kb on - strand, within argF at 282.530 kb on + strand, within argF at 282.538 kb on - strand, within argF at 282.538 kb on - strand, within argF at 282.538 kb on - strand, within argF at 282.585 kb on + strand, within argF at 282.639 kb on + strand, within argF at 283.043 kb on + strand, within argF at 283.044 kb on + strand, within argF at 283.117 kb on + strand, within argF at 283.127 kb on + strand, within argF at 283.135 kb on - strand, within argF at 283.174 kb on - strand at 283.174 kb on - strand at 283.307 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 280,553 + -1.7 280,571 + -3.9 280,578 + -3.5 280,579 - -4.1 280,579 - -2.1 280,586 - -3.7 280,586 - -4.3 280,615 - -3.7 280,623 - argC SO0275 0.10 -2.9 280,662 + argC SO0275 0.14 -6.0 280,670 - argC SO0275 0.15 -4.6 280,670 - argC SO0275 0.15 -3.5 280,674 + argC SO0275 0.15 -2.8 280,674 + argC SO0275 0.15 -5.6 280,674 + argC SO0275 0.15 -5.6 280,674 + argC SO0275 0.15 -5.9 280,674 + argC SO0275 0.15 -4.3 280,682 - argC SO0275 0.16 -5.4 280,689 + -0.2 280,697 - argC SO0275 0.18 -2.9 280,704 - argC SO0275 0.18 -1.8 280,755 - argC SO0275 0.24 -4.3 280,757 + argC SO0275 0.24 -4.1 280,820 - argC SO0275 0.30 -5.6 280,837 + argC SO0275 0.32 -3.7 280,882 - argC SO0275 0.36 -5.1 280,977 + argC SO0275 0.46 -2.9 281,045 - argC SO0275 0.53 -2.1 281,047 + argC SO0275 0.53 -3.1 281,047 + argC SO0275 0.53 -4.5 281,055 - argC SO0275 0.54 -3.9 281,067 - argC SO0275 0.55 -3.2 281,080 - argC SO0275 0.57 -1.4 281,125 + argC SO0275 0.61 -2.7 281,125 + argC SO0275 0.61 -3.0 281,133 - argC SO0275 0.62 -2.9 281,133 - argC SO0275 0.62 -4.3 281,142 + argC SO0275 0.63 -2.0 281,250 - argC SO0275 0.74 -5.7 281,255 + argC SO0275 0.75 -1.0 281,255 + argC SO0275 0.75 -4.8 281,309 - argC SO0275 0.80 -3.7 281,359 + argC SO0275 0.85 -3.9 281,421 + -5.7 281,469 + -3.2 281,732 - argB SO0276 0.25 -4.7 281,880 + argB SO0276 0.44 -1.0 281,880 + argB SO0276 0.44 -4.0 282,002 + argB SO0276 0.60 -4.9 282,010 - argB SO0276 0.61 -3.8 282,103 + argB SO0276 0.72 -3.9 282,133 + argB SO0276 0.76 -4.9 282,214 + argB SO0276 0.87 -3.6 282,455 - argF SO0277 0.14 -4.3 282,530 + argF SO0277 0.22 -3.5 282,538 - argF SO0277 0.23 -4.4 282,538 - argF SO0277 0.23 -5.5 282,538 - argF SO0277 0.23 -2.7 282,585 + argF SO0277 0.28 -3.7 282,639 + argF SO0277 0.34 -1.8 283,043 + argF SO0277 0.79 -3.3 283,044 + argF SO0277 0.79 -3.5 283,117 + argF SO0277 0.87 -4.3 283,127 + argF SO0277 0.88 +0.8 283,135 - argF SO0277 0.89 -5.5 283,174 - -6.1 283,174 - -4.4 283,307 - -5.3
Or see this region's nucleotide sequence