Experiment: Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and ruvA are separated by 17 nucleotides ruvA and ruvC overlap by 4 nucleotides ruvC and Sama_1842 are separated by 3 nucleotides Sama_1842 and aspS are separated by 123 nucleotides
Sama_1839: ruvB - Holliday junction DNA helicase RuvB (RefSeq), at 2,240,347 to 2,241,351
ruvB
Sama_1840: ruvA - Holliday junction DNA helicase RuvA (RefSeq), at 2,241,369 to 2,241,986
ruvA
Sama_1841: ruvC - Holliday junction resolvase (RefSeq), at 2,241,983 to 2,242,504
ruvC
Sama_1842: Sama_1842 - hypothetical protein (RefSeq), at 2,242,508 to 2,243,254
_1842
Sama_1843: aspS - aspartyl-tRNA synthetase (RefSeq), at 2,243,378 to 2,245,153
aspS
Position (kb)
2241
2242
2243 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2240.998 kb on + strand, within ruvB at 2241.150 kb on + strand, within ruvB at 2241.150 kb on + strand, within ruvB at 2241.151 kb on - strand, within ruvB at 2241.175 kb on - strand, within ruvB at 2241.180 kb on + strand, within ruvB at 2241.181 kb on - strand, within ruvB at 2241.181 kb on - strand, within ruvB at 2241.181 kb on - strand, within ruvB at 2241.272 kb on - strand at 2241.272 kb on - strand at 2241.355 kb on - strand at 2241.371 kb on - strand at 2241.371 kb on - strand at 2241.399 kb on - strand at 2241.692 kb on + strand, within ruvA at 2241.931 kb on - strand at 2241.982 kb on - strand at 2241.991 kb on - strand at 2241.991 kb on - strand at 2242.406 kb on - strand, within ruvC at 2242.422 kb on + strand, within ruvC at 2242.422 kb on + strand, within ruvC at 2242.549 kb on + strand at 2242.554 kb on + strand at 2242.555 kb on - strand at 2242.555 kb on - strand at 2242.555 kb on - strand at 2242.730 kb on - strand, within Sama_1842 at 2242.730 kb on - strand, within Sama_1842 at 2242.735 kb on - strand, within Sama_1842 at 2242.735 kb on - strand, within Sama_1842 at 2242.735 kb on - strand, within Sama_1842 at 2242.836 kb on - strand, within Sama_1842 at 2242.836 kb on - strand, within Sama_1842 at 2242.901 kb on - strand, within Sama_1842 at 2242.901 kb on - strand, within Sama_1842 at 2242.901 kb on - strand, within Sama_1842 at 2243.075 kb on - strand, within Sama_1842 at 2243.255 kb on - strand at 2243.277 kb on + strand at 2243.377 kb on - strand at 2243.377 kb on - strand at 2243.377 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.005 mg/ml remove 2,240,998 + ruvB Sama_1839 0.65 -1.3 2,241,150 + ruvB Sama_1839 0.80 -2.2 2,241,150 + ruvB Sama_1839 0.80 -1.7 2,241,151 - ruvB Sama_1839 0.80 -2.7 2,241,175 - ruvB Sama_1839 0.82 -0.3 2,241,180 + ruvB Sama_1839 0.83 +0.7 2,241,181 - ruvB Sama_1839 0.83 -0.3 2,241,181 - ruvB Sama_1839 0.83 -0.9 2,241,181 - ruvB Sama_1839 0.83 -0.3 2,241,272 - -1.7 2,241,272 - -1.3 2,241,355 - -2.8 2,241,371 - -2.5 2,241,371 - -1.6 2,241,399 - -1.9 2,241,692 + ruvA Sama_1840 0.52 -2.3 2,241,931 - -0.3 2,241,982 - -0.3 2,241,991 - -0.3 2,241,991 - -3.3 2,242,406 - ruvC Sama_1841 0.81 -0.3 2,242,422 + ruvC Sama_1841 0.84 +0.7 2,242,422 + ruvC Sama_1841 0.84 +0.1 2,242,549 + -1.3 2,242,554 + +0.1 2,242,555 - -0.5 2,242,555 - -0.6 2,242,555 - +0.1 2,242,730 - Sama_1842 0.30 -0.7 2,242,730 - Sama_1842 0.30 -1.8 2,242,735 - Sama_1842 0.30 +0.1 2,242,735 - Sama_1842 0.30 -2.2 2,242,735 - Sama_1842 0.30 -1.2 2,242,836 - Sama_1842 0.44 -1.3 2,242,836 - Sama_1842 0.44 -1.2 2,242,901 - Sama_1842 0.53 -0.9 2,242,901 - Sama_1842 0.53 -1.7 2,242,901 - Sama_1842 0.53 +0.7 2,243,075 - Sama_1842 0.76 +0.3 2,243,255 - -2.6 2,243,277 + -2.2 2,243,377 - +1.1 2,243,377 - -0.8 2,243,377 - +0.9
Or see this region's nucleotide sequence