Strain Fitness in Shewanella amazonensis SB2B around Sama_0015

Experiment: Cisplatin 0.005 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSama_0014 and recF are separated by 27 nucleotidesrecF and Sama_0016 are separated by 92 nucleotides Sama_0014: Sama_0014 - DNA-directed DNA polymerase (RefSeq), at 12,168 to 13,268 _0014 Sama_0015: recF - recombination protein F (RefSeq), at 13,296 to 14,378 recF Sama_0016: Sama_0016 - DNA topoisomerase (ATP-hydrolyzing) (RefSeq), at 14,471 to 16,888 _0016 Position (kb) 13 14 15Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 13.266 kb on + strandat 13.266 kb on + strandat 13.266 kb on + strandat 13.266 kb on + strandat 13.267 kb on - strandat 13.295 kb on + strandat 13.295 kb on + strandat 13.296 kb on - strandat 13.296 kb on - strandat 13.296 kb on - strandat 13.334 kb on + strandat 13.335 kb on - strandat 13.335 kb on - strandat 13.380 kb on + strandat 13.380 kb on + strandat 13.381 kb on - strandat 13.425 kb on + strand, within recFat 13.425 kb on + strand, within recFat 13.425 kb on + strand, within recFat 13.425 kb on + strand, within recFat 13.425 kb on + strand, within recFat 13.452 kb on - strand, within recFat 13.452 kb on - strand, within recFat 13.541 kb on + strand, within recFat 13.542 kb on - strand, within recFat 13.542 kb on - strand, within recFat 13.630 kb on + strand, within recFat 13.630 kb on + strand, within recFat 13.630 kb on + strand, within recFat 13.630 kb on + strand, within recFat 13.630 kb on + strand, within recFat 13.630 kb on + strand, within recFat 13.630 kb on + strand, within recFat 13.630 kb on + strand, within recFat 13.631 kb on - strand, within recFat 13.631 kb on - strand, within recFat 13.631 kb on - strand, within recFat 13.631 kb on - strand, within recFat 13.635 kb on - strand, within recFat 13.635 kb on - strand, within recFat 13.635 kb on - strand, within recFat 13.635 kb on - strand, within recFat 13.647 kb on - strand, within recFat 13.701 kb on - strand, within recFat 13.701 kb on - strand, within recFat 13.754 kb on + strand, within recFat 13.754 kb on + strand, within recFat 13.755 kb on - strand, within recFat 13.838 kb on - strand, within recFat 13.841 kb on + strand, within recFat 13.867 kb on + strand, within recFat 13.867 kb on + strand, within recFat 13.878 kb on + strand, within recFat 13.878 kb on + strand, within recFat 13.878 kb on + strand, within recFat 13.878 kb on + strand, within recFat 13.878 kb on + strand, within recFat 13.879 kb on - strand, within recFat 13.879 kb on - strand, within recFat 13.879 kb on - strand, within recFat 13.882 kb on + strand, within recFat 13.882 kb on + strand, within recFat 13.882 kb on + strand, within recFat 13.882 kb on + strand, within recFat 13.883 kb on - strand, within recFat 13.883 kb on - strand, within recFat 13.883 kb on - strand, within recFat 13.883 kb on - strand, within recFat 13.883 kb on - strand, within recFat 13.883 kb on - strand, within recFat 13.904 kb on + strand, within recFat 13.904 kb on + strand, within recFat 13.904 kb on + strand, within recFat 13.910 kb on + strand, within recFat 13.910 kb on + strand, within recFat 13.912 kb on + strand, within recFat 13.912 kb on + strand, within recFat 14.007 kb on + strand, within recFat 14.026 kb on + strand, within recFat 14.033 kb on + strand, within recFat 14.033 kb on + strand, within recFat 14.051 kb on + strand, within recFat 14.051 kb on + strand, within recFat 14.078 kb on + strand, within recFat 14.078 kb on + strand, within recFat 14.122 kb on + strand, within recFat 14.162 kb on + strand, within recFat 14.162 kb on + strand, within recFat 14.162 kb on + strand, within recFat 14.190 kb on + strand, within recFat 14.190 kb on + strand, within recFat 14.190 kb on + strand, within recFat 14.190 kb on + strand, within recFat 14.190 kb on + strand, within recFat 14.257 kb on + strand, within recFat 14.257 kb on + strand, within recFat 14.257 kb on + strand, within recFat 14.261 kb on + strand, within recFat 14.261 kb on + strand, within recFat 14.347 kb on + strandat 14.347 kb on + strandat 14.347 kb on + strandat 14.372 kb on + strandat 14.372 kb on + strandat 14.372 kb on + strandat 14.372 kb on + strandat 14.376 kb on + strandat 14.376 kb on + strandat 14.376 kb on + strandat 14.376 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.005 mg/ml
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13,266 + -1.1
13,266 + -1.7
13,266 + -3.3
13,266 + -5.0
13,267 - -0.9
13,295 + -2.8
13,295 + -0.7
13,296 - -3.3
13,296 - -1.1
13,296 - -3.8
13,334 + -3.1
13,335 - -1.9
13,335 - -2.5
13,380 + -3.1
13,380 + -2.9
13,381 - -3.8
13,425 + recF Sama_0015 0.12 -2.8
13,425 + recF Sama_0015 0.12 -3.5
13,425 + recF Sama_0015 0.12 -1.1
13,425 + recF Sama_0015 0.12 -2.9
13,425 + recF Sama_0015 0.12 -0.9
13,452 - recF Sama_0015 0.14 -2.1
13,452 - recF Sama_0015 0.14 -5.7
13,541 + recF Sama_0015 0.23 -4.4
13,542 - recF Sama_0015 0.23 -2.3
13,542 - recF Sama_0015 0.23 -3.0
13,630 + recF Sama_0015 0.31 -3.0
13,630 + recF Sama_0015 0.31 -5.1
13,630 + recF Sama_0015 0.31 +0.9
13,630 + recF Sama_0015 0.31 -2.8
13,630 + recF Sama_0015 0.31 -0.9
13,630 + recF Sama_0015 0.31 -2.5
13,630 + recF Sama_0015 0.31 -4.0
13,630 + recF Sama_0015 0.31 -1.9
13,631 - recF Sama_0015 0.31 -2.5
13,631 - recF Sama_0015 0.31 -4.1
13,631 - recF Sama_0015 0.31 -1.3
13,631 - recF Sama_0015 0.31 -1.4
13,635 - recF Sama_0015 0.31 -1.2
13,635 - recF Sama_0015 0.31 -2.6
13,635 - recF Sama_0015 0.31 -3.2
13,635 - recF Sama_0015 0.31 -3.6
13,647 - recF Sama_0015 0.32 -2.9
13,701 - recF Sama_0015 0.37 -1.7
13,701 - recF Sama_0015 0.37 -3.4
13,754 + recF Sama_0015 0.42 -3.8
13,754 + recF Sama_0015 0.42 -2.1
13,755 - recF Sama_0015 0.42 -2.1
13,838 - recF Sama_0015 0.50 -1.5
13,841 + recF Sama_0015 0.50 -4.2
13,867 + recF Sama_0015 0.53 -3.9
13,867 + recF Sama_0015 0.53 -3.7
13,878 + recF Sama_0015 0.54 -3.2
13,878 + recF Sama_0015 0.54 -2.8
13,878 + recF Sama_0015 0.54 -3.7
13,878 + recF Sama_0015 0.54 -3.3
13,878 + recF Sama_0015 0.54 -4.7
13,879 - recF Sama_0015 0.54 -0.7
13,879 - recF Sama_0015 0.54 -3.1
13,879 - recF Sama_0015 0.54 -3.7
13,882 + recF Sama_0015 0.54 -0.9
13,882 + recF Sama_0015 0.54 +0.3
13,882 + recF Sama_0015 0.54 -3.0
13,882 + recF Sama_0015 0.54 -4.3
13,883 - recF Sama_0015 0.54 -1.1
13,883 - recF Sama_0015 0.54 -2.0
13,883 - recF Sama_0015 0.54 -3.4
13,883 - recF Sama_0015 0.54 -1.9
13,883 - recF Sama_0015 0.54 -2.5
13,883 - recF Sama_0015 0.54 -1.9
13,904 + recF Sama_0015 0.56 -2.0
13,904 + recF Sama_0015 0.56 -1.1
13,904 + recF Sama_0015 0.56 -2.5
13,910 + recF Sama_0015 0.57 -1.6
13,910 + recF Sama_0015 0.57 -1.7
13,912 + recF Sama_0015 0.57 -2.6
13,912 + recF Sama_0015 0.57 -2.6
14,007 + recF Sama_0015 0.66 -4.3
14,026 + recF Sama_0015 0.67 -2.1
14,033 + recF Sama_0015 0.68 -1.5
14,033 + recF Sama_0015 0.68 -2.3
14,051 + recF Sama_0015 0.70 -3.1
14,051 + recF Sama_0015 0.70 -3.3
14,078 + recF Sama_0015 0.72 -1.1
14,078 + recF Sama_0015 0.72 -1.7
14,122 + recF Sama_0015 0.76 -4.0
14,162 + recF Sama_0015 0.80 -2.5
14,162 + recF Sama_0015 0.80 -1.7
14,162 + recF Sama_0015 0.80 -2.4
14,190 + recF Sama_0015 0.83 -2.1
14,190 + recF Sama_0015 0.83 -1.1
14,190 + recF Sama_0015 0.83 -2.8
14,190 + recF Sama_0015 0.83 -2.1
14,190 + recF Sama_0015 0.83 -1.5
14,257 + recF Sama_0015 0.89 -3.9
14,257 + recF Sama_0015 0.89 -0.1
14,257 + recF Sama_0015 0.89 -2.4
14,261 + recF Sama_0015 0.89 -4.2
14,261 + recF Sama_0015 0.89 -3.1
14,347 + -4.7
14,347 + -3.7
14,347 + -3.9
14,372 + +1.2
14,372 + -0.5
14,372 + -1.1
14,372 + -1.5
14,376 + -0.1
14,376 + -1.2
14,376 + -0.6
14,376 + -0.3

Or see this region's nucleotide sequence