Strain Fitness in Shewanella oneidensis MR-1 around SO_t044

Experiment: L-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO2370 and tRNA-Ser-5 are separated by 692 nucleotidestRNA-Ser-5 and SO2373 are separated by 80 nucleotides SO2370: SO2370 - site-specific recombinase, phage integrase family (NCBI ptt file), at 2,476,011 to 2,476,877 SO2370 SO_t044: tRNA-Ser-5 - tRNA-Ser (RefSeq), at 2,477,570 to 2,477,660 tRNA-Ser-5 SO2373: SO2373 - drug resistance transporter, Bcr/CflA family protein (NCBI ptt file), at 2,477,741 to 2,479,012 SO2373 Position (kb) 2477 2478Strain fitness (log2 ratio) -1 0 1at 2476.716 kb on + strand, within SO2370at 2476.872 kb on + strandat 2476.893 kb on + strandat 2476.898 kb on + strandat 2476.901 kb on - strandat 2476.906 kb on - strandat 2476.957 kb on + strandat 2477.100 kb on + strandat 2477.137 kb on - strandat 2477.233 kb on - strandat 2477.285 kb on + strandat 2477.295 kb on + strandat 2477.414 kb on - strandat 2477.414 kb on - strandat 2477.696 kb on + strandat 2477.836 kb on - strandat 2477.893 kb on - strand, within SO2373at 2478.031 kb on - strand, within SO2373at 2478.133 kb on + strand, within SO2373at 2478.166 kb on - strand, within SO2373at 2478.191 kb on - strand, within SO2373at 2478.558 kb on + strand, within SO2373

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Alanine (N)
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2,476,716 + SO2370 0.81 -1.2
2,476,872 + +0.3
2,476,893 + -1.3
2,476,898 + +0.7
2,476,901 - +0.2
2,476,906 - -1.7
2,476,957 + +0.1
2,477,100 + -0.9
2,477,137 - -1.0
2,477,233 - -0.8
2,477,285 + +0.3
2,477,295 + +0.0
2,477,414 - -0.4
2,477,414 - +0.0
2,477,696 + -0.2
2,477,836 - -0.3
2,477,893 - SO2373 0.12 -0.1
2,478,031 - SO2373 0.23 -1.0
2,478,133 + SO2373 0.31 +1.0
2,478,166 - SO2373 0.33 -1.4
2,478,191 - SO2373 0.35 +0.9
2,478,558 + SO2373 0.64 -0.3

Or see this region's nucleotide sequence