Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysT-1 and cysW-1 are separated by 9 nucleotides cysW-1 and cysA-1 overlap by 4 nucleotides cysA-1 and SO3603 are separated by 122 nucleotides
SO3600: cysT-1 - sulfate ABC transporter, permease protein (NCBI ptt file), at 3,762,243 to 3,763,088
cysT-1
SO3601: cysW-1 - sulfate ABC transporter, permease protein (NCBI ptt file), at 3,763,098 to 3,763,967
cysW-1
SO3602: cysA-1 - sulfate ABC transporter, ATP-binding protein (NCBI ptt file), at 3,763,964 to 3,765,094
cysA-1
SO3603: SO3603 - secretion protein, HlyD family (NCBI ptt file), at 3,765,217 to 3,766,353
SO3603
Position (kb)
3763
3764
3765
3766 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3763.016 kb on - strand at 3763.039 kb on + strand at 3763.040 kb on + strand at 3763.046 kb on + strand at 3763.105 kb on + strand at 3763.167 kb on - strand at 3763.197 kb on + strand, within cysW-1 at 3763.269 kb on + strand, within cysW-1 at 3763.448 kb on - strand, within cysW-1 at 3763.450 kb on + strand, within cysW-1 at 3763.458 kb on - strand, within cysW-1 at 3763.458 kb on - strand, within cysW-1 at 3763.483 kb on + strand, within cysW-1 at 3763.483 kb on + strand, within cysW-1 at 3763.491 kb on - strand, within cysW-1 at 3763.674 kb on - strand, within cysW-1 at 3763.705 kb on - strand, within cysW-1 at 3763.721 kb on + strand, within cysW-1 at 3763.789 kb on - strand, within cysW-1 at 3763.837 kb on - strand, within cysW-1 at 3763.839 kb on + strand, within cysW-1 at 3763.849 kb on + strand, within cysW-1 at 3764.351 kb on + strand, within cysA-1 at 3764.364 kb on - strand, within cysA-1 at 3764.364 kb on - strand, within cysA-1 at 3764.371 kb on + strand, within cysA-1 at 3764.379 kb on - strand, within cysA-1 at 3764.379 kb on - strand, within cysA-1 at 3764.382 kb on + strand, within cysA-1 at 3764.466 kb on + strand, within cysA-1 at 3764.481 kb on - strand, within cysA-1 at 3764.544 kb on - strand, within cysA-1 at 3764.569 kb on - strand, within cysA-1 at 3764.570 kb on - strand, within cysA-1 at 3764.582 kb on - strand, within cysA-1 at 3764.604 kb on + strand, within cysA-1 at 3764.632 kb on + strand, within cysA-1 at 3764.632 kb on + strand, within cysA-1 at 3764.719 kb on + strand, within cysA-1 at 3764.818 kb on + strand, within cysA-1 at 3764.991 kb on - strand at 3764.991 kb on - strand at 3765.082 kb on - strand at 3765.104 kb on + strand at 3765.104 kb on + strand at 3765.181 kb on - strand at 3765.204 kb on + strand at 3765.264 kb on + strand at 3765.277 kb on - strand at 3765.285 kb on - strand at 3765.350 kb on - strand, within SO3603 at 3765.461 kb on + strand, within SO3603 at 3765.476 kb on + strand, within SO3603 at 3765.527 kb on - strand, within SO3603 at 3765.527 kb on - strand, within SO3603 at 3765.616 kb on + strand, within SO3603 at 3765.623 kb on + strand, within SO3603 at 3765.688 kb on - strand, within SO3603 at 3765.749 kb on + strand, within SO3603 at 3766.015 kb on + strand, within SO3603 at 3766.023 kb on - strand, within SO3603 at 3766.033 kb on - strand, within SO3603 at 3766.074 kb on - strand, within SO3603
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 3,763,016 - -2.8 3,763,039 + -2.3 3,763,040 + -1.4 3,763,046 + -3.0 3,763,105 + -1.8 3,763,167 - -3.0 3,763,197 + cysW-1 SO3601 0.11 -2.4 3,763,269 + cysW-1 SO3601 0.20 -3.1 3,763,448 - cysW-1 SO3601 0.40 -1.2 3,763,450 + cysW-1 SO3601 0.40 -1.8 3,763,458 - cysW-1 SO3601 0.41 -2.8 3,763,458 - cysW-1 SO3601 0.41 -2.0 3,763,483 + cysW-1 SO3601 0.44 -2.1 3,763,483 + cysW-1 SO3601 0.44 -1.2 3,763,491 - cysW-1 SO3601 0.45 -3.7 3,763,674 - cysW-1 SO3601 0.66 -3.0 3,763,705 - cysW-1 SO3601 0.70 -3.4 3,763,721 + cysW-1 SO3601 0.72 -1.7 3,763,789 - cysW-1 SO3601 0.79 -2.8 3,763,837 - cysW-1 SO3601 0.85 -2.6 3,763,839 + cysW-1 SO3601 0.85 -3.9 3,763,849 + cysW-1 SO3601 0.86 -2.1 3,764,351 + cysA-1 SO3602 0.34 -3.3 3,764,364 - cysA-1 SO3602 0.35 -1.8 3,764,364 - cysA-1 SO3602 0.35 -1.5 3,764,371 + cysA-1 SO3602 0.36 -1.9 3,764,379 - cysA-1 SO3602 0.37 -3.2 3,764,379 - cysA-1 SO3602 0.37 -1.3 3,764,382 + cysA-1 SO3602 0.37 -1.5 3,764,466 + cysA-1 SO3602 0.44 -1.8 3,764,481 - cysA-1 SO3602 0.46 -1.1 3,764,544 - cysA-1 SO3602 0.51 -1.5 3,764,569 - cysA-1 SO3602 0.53 -2.9 3,764,570 - cysA-1 SO3602 0.54 -2.7 3,764,582 - cysA-1 SO3602 0.55 -4.2 3,764,604 + cysA-1 SO3602 0.57 -1.7 3,764,632 + cysA-1 SO3602 0.59 -2.2 3,764,632 + cysA-1 SO3602 0.59 -2.9 3,764,719 + cysA-1 SO3602 0.67 -3.6 3,764,818 + cysA-1 SO3602 0.76 -1.0 3,764,991 - -1.4 3,764,991 - -1.7 3,765,082 - -0.1 3,765,104 + -0.7 3,765,104 + +0.1 3,765,181 - +2.0 3,765,204 + +1.5 3,765,264 + +0.0 3,765,277 - +1.0 3,765,285 - -0.2 3,765,350 - SO3603 0.12 -0.5 3,765,461 + SO3603 0.21 +0.3 3,765,476 + SO3603 0.23 -0.6 3,765,527 - SO3603 0.27 +0.4 3,765,527 - SO3603 0.27 -0.3 3,765,616 + SO3603 0.35 -0.9 3,765,623 + SO3603 0.36 +1.2 3,765,688 - SO3603 0.41 +0.7 3,765,749 + SO3603 0.47 +0.4 3,766,015 + SO3603 0.70 -0.9 3,766,023 - SO3603 0.71 -0.2 3,766,033 - SO3603 0.72 -0.2 3,766,074 - SO3603 0.75 +0.5
Or see this region's nucleotide sequence