Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ansA and SO2422 are separated by 70 nucleotides SO2422 and SO2423 overlap by 4 nucleotides SO2423 and SO2424 are separated by 8 nucleotides SO2424 and SO2426 are separated by 181 nucleotides
SO2421: ansA - L-asparaginase I (NCBI ptt file), at 2,530,903 to 2,531,916
ansA
SO2422: SO2422 - conserved hypothetical protein (NCBI ptt file), at 2,531,987 to 2,532,268
SO2422
SO2423: SO2423 - hypothetical protein (NCBI ptt file), at 2,532,265 to 2,532,582
SO2423
SO2424: SO2424 - zinc carboxypeptidase domain protein (NCBI ptt file), at 2,532,591 to 2,533,718
SO2424
SO2426: SO2426 - DNA-binding response regulator (NCBI ptt file), at 2,533,900 to 2,534,613
SO2426
Position (kb)
2532
2533
2534 Strain fitness (log2 ratio)
-1
0
1
2 at 2531.632 kb on - strand, within ansA at 2531.969 kb on + strand at 2531.977 kb on - strand at 2531.977 kb on - strand at 2532.056 kb on - strand, within SO2422 at 2532.172 kb on - strand, within SO2422 at 2532.217 kb on - strand, within SO2422 at 2532.226 kb on - strand, within SO2422 at 2532.272 kb on - strand at 2532.272 kb on - strand at 2532.353 kb on - strand, within SO2423 at 2532.420 kb on - strand, within SO2423 at 2532.644 kb on + strand at 2532.759 kb on - strand, within SO2424 at 2532.837 kb on + strand, within SO2424 at 2532.845 kb on - strand, within SO2424 at 2532.845 kb on - strand, within SO2424 at 2532.869 kb on + strand, within SO2424 at 2532.896 kb on + strand, within SO2424 at 2533.001 kb on + strand, within SO2424 at 2533.001 kb on + strand, within SO2424 at 2533.009 kb on - strand, within SO2424 at 2533.193 kb on - strand, within SO2424 at 2533.226 kb on + strand, within SO2424 at 2533.351 kb on + strand, within SO2424 at 2533.388 kb on + strand, within SO2424 at 2533.529 kb on - strand, within SO2424 at 2533.564 kb on - strand, within SO2424 at 2533.747 kb on + strand at 2533.747 kb on + strand at 2533.755 kb on - strand at 2533.920 kb on - strand at 2534.387 kb on + strand, within SO2426 at 2534.473 kb on - strand, within SO2426 at 2534.516 kb on + strand, within SO2426 at 2534.516 kb on + strand, within SO2426 at 2534.688 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,531,632 - ansA SO2421 0.72 +1.4 2,531,969 + -0.5 2,531,977 - +0.7 2,531,977 - +0.9 2,532,056 - SO2422 0.24 +0.0 2,532,172 - SO2422 0.66 -0.6 2,532,217 - SO2422 0.82 +0.1 2,532,226 - SO2422 0.85 -0.9 2,532,272 - +0.4 2,532,272 - -0.3 2,532,353 - SO2423 0.28 -0.2 2,532,420 - SO2423 0.49 -0.7 2,532,644 + +1.0 2,532,759 - SO2424 0.15 +0.4 2,532,837 + SO2424 0.22 +1.8 2,532,845 - SO2424 0.23 +0.2 2,532,845 - SO2424 0.23 -0.6 2,532,869 + SO2424 0.25 +1.3 2,532,896 + SO2424 0.27 +1.0 2,533,001 + SO2424 0.36 +1.3 2,533,001 + SO2424 0.36 +0.2 2,533,009 - SO2424 0.37 -0.7 2,533,193 - SO2424 0.53 +0.3 2,533,226 + SO2424 0.56 +0.5 2,533,351 + SO2424 0.67 +1.0 2,533,388 + SO2424 0.71 +1.9 2,533,529 - SO2424 0.83 -0.3 2,533,564 - SO2424 0.86 -0.1 2,533,747 + +0.4 2,533,747 + +0.0 2,533,755 - +1.1 2,533,920 - +0.1 2,534,387 + SO2426 0.68 +1.0 2,534,473 - SO2426 0.80 +1.4 2,534,516 + SO2426 0.86 +1.1 2,534,516 + SO2426 0.86 +0.5 2,534,688 - +0.4
Or see this region's nucleotide sequence