Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sapA and pspF are separated by 117 nucleotides pspF and pspA are separated by 177 nucleotides pspA and pspB are separated by 17 nucleotides
SO1805: sapA - peptide ABC transporter, periplasmic peptide-binding protein (NCBI ptt file), at 1,894,864 to 1,896,441
sapA
SO1806: pspF - psp operon transcriptional activator (NCBI ptt file), at 1,896,559 to 1,897,650
pspF
SO1807: pspA - phage shock protein A (NCBI ptt file), at 1,897,828 to 1,898,511
pspA
SO1808: pspB - phage shock protein B (NCBI ptt file), at 1,898,529 to 1,898,768
pspB
Position (kb)
1896
1897
1898 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1895.567 kb on - strand, within sapA at 1896.031 kb on - strand, within sapA at 1896.197 kb on - strand, within sapA at 1896.222 kb on - strand, within sapA at 1896.543 kb on + strand at 1896.603 kb on + strand at 1896.611 kb on - strand at 1896.740 kb on + strand, within pspF at 1896.748 kb on + strand, within pspF at 1896.748 kb on - strand, within pspF at 1896.765 kb on + strand, within pspF at 1896.817 kb on + strand, within pspF at 1896.903 kb on + strand, within pspF at 1896.908 kb on + strand, within pspF at 1896.967 kb on + strand, within pspF at 1896.989 kb on - strand, within pspF at 1897.037 kb on + strand, within pspF at 1897.052 kb on + strand, within pspF at 1897.060 kb on - strand, within pspF at 1897.060 kb on - strand, within pspF at 1897.081 kb on + strand, within pspF at 1897.165 kb on - strand, within pspF at 1897.353 kb on + strand, within pspF at 1897.371 kb on - strand, within pspF at 1897.443 kb on + strand, within pspF at 1897.497 kb on - strand, within pspF at 1897.585 kb on + strand at 1897.592 kb on + strand at 1897.600 kb on - strand at 1897.857 kb on + strand at 1897.863 kb on + strand at 1897.891 kb on + strand at 1897.947 kb on + strand, within pspA at 1898.142 kb on - strand, within pspA at 1898.253 kb on + strand, within pspA at 1898.512 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,895,567 - sapA SO1805 0.45 +3.1 1,896,031 - sapA SO1805 0.74 +1.0 1,896,197 - sapA SO1805 0.84 +2.0 1,896,222 - sapA SO1805 0.86 +0.5 1,896,543 + +2.7 1,896,603 + -0.1 1,896,611 - +0.2 1,896,740 + pspF SO1806 0.17 +1.3 1,896,748 + pspF SO1806 0.17 -0.2 1,896,748 - pspF SO1806 0.17 +0.1 1,896,765 + pspF SO1806 0.19 +0.2 1,896,817 + pspF SO1806 0.24 +2.2 1,896,903 + pspF SO1806 0.32 -0.3 1,896,908 + pspF SO1806 0.32 -0.4 1,896,967 + pspF SO1806 0.37 -0.2 1,896,989 - pspF SO1806 0.39 +0.9 1,897,037 + pspF SO1806 0.44 +0.9 1,897,052 + pspF SO1806 0.45 +0.7 1,897,060 - pspF SO1806 0.46 +0.3 1,897,060 - pspF SO1806 0.46 +1.8 1,897,081 + pspF SO1806 0.48 +3.5 1,897,165 - pspF SO1806 0.55 +1.1 1,897,353 + pspF SO1806 0.73 +0.4 1,897,371 - pspF SO1806 0.74 +1.0 1,897,443 + pspF SO1806 0.81 -0.0 1,897,497 - pspF SO1806 0.86 -0.1 1,897,585 + +0.7 1,897,592 + +0.4 1,897,600 - -1.1 1,897,857 + -1.8 1,897,863 + -0.9 1,897,891 + -2.4 1,897,947 + pspA SO1807 0.17 +0.4 1,898,142 - pspA SO1807 0.46 -0.3 1,898,253 + pspA SO1807 0.62 -1.0 1,898,512 + +0.7
Or see this region's nucleotide sequence