Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pckA and hslO are separated by 201 nucleotides hslO and hslR are separated by 25 nucleotides hslR and gspC are separated by 251 nucleotides
SO0162: pckA - phosphoenolpyruvate carboxykinase (ATP) (NCBI ptt file), at 168,071 to 169,612
pckA
SO0163: hslO - chaperonin HslO (NCBI ptt file), at 169,814 to 170,674
hslO
SO0164: hslR - heat shock protein 15 (NCBI ptt file), at 170,700 to 171,098
hslR
SO0165: gspC - general secretion pathway protein C (NCBI ptt file), at 171,350 to 172,276
gspC
Position (kb) 169
170
171 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 168.874 kb on + strand, within pckA at 168.874 kb on + strand, within pckA at 168.876 kb on + strand, within pckA at 168.882 kb on - strand, within pckA at 168.918 kb on - strand, within pckA at 168.977 kb on + strand, within pckA at 168.985 kb on - strand, within pckA at 168.985 kb on - strand, within pckA at 169.001 kb on + strand, within pckA at 169.008 kb on + strand, within pckA at 169.013 kb on - strand, within pckA at 169.174 kb on - strand, within pckA at 169.200 kb on + strand, within pckA at 169.222 kb on + strand, within pckA at 169.247 kb on + strand, within pckA at 169.267 kb on - strand, within pckA at 169.337 kb on - strand, within pckA at 169.352 kb on + strand, within pckA at 169.409 kb on + strand, within pckA at 169.481 kb on + strand at 169.487 kb on + strand at 169.564 kb on + strand at 169.567 kb on + strand at 169.572 kb on + strand at 169.586 kb on - strand at 169.623 kb on + strand at 169.628 kb on + strand at 169.742 kb on + strand at 169.742 kb on + strand at 169.780 kb on + strand at 169.780 kb on + strand at 169.785 kb on + strand at 169.848 kb on - strand at 169.912 kb on - strand, within hslO at 169.945 kb on - strand, within hslO at 169.972 kb on + strand, within hslO at 169.987 kb on + strand, within hslO at 170.009 kb on + strand, within hslO at 170.060 kb on + strand, within hslO at 170.060 kb on + strand, within hslO at 170.060 kb on + strand, within hslO at 170.060 kb on + strand, within hslO at 170.060 kb on + strand, within hslO at 170.060 kb on + strand, within hslO at 170.068 kb on - strand, within hslO at 170.068 kb on - strand, within hslO at 170.068 kb on - strand, within hslO at 170.068 kb on - strand, within hslO at 170.068 kb on - strand, within hslO at 170.068 kb on - strand, within hslO at 170.077 kb on + strand, within hslO at 170.085 kb on - strand, within hslO at 170.123 kb on + strand, within hslO at 170.188 kb on - strand, within hslO at 170.213 kb on + strand, within hslO at 170.241 kb on - strand, within hslO at 170.242 kb on + strand, within hslO at 170.250 kb on - strand, within hslO at 170.250 kb on - strand, within hslO at 170.338 kb on + strand, within hslO at 170.369 kb on + strand, within hslO at 170.377 kb on - strand, within hslO at 170.549 kb on + strand, within hslO at 170.678 kb on - strand at 170.772 kb on - strand, within hslR at 170.984 kb on - strand, within hslR at 170.984 kb on - strand, within hslR at 171.080 kb on + strand at 171.088 kb on - strand at 171.139 kb on + strand at 171.163 kb on + strand at 171.171 kb on + strand at 171.204 kb on - strand at 171.281 kb on + strand at 171.305 kb on - strand at 171.329 kb on + strand at 171.361 kb on + strand at 171.387 kb on - strand at 171.453 kb on - strand, within gspC at 171.513 kb on - strand, within gspC at 171.518 kb on - strand, within gspC at 171.520 kb on + strand, within gspC at 171.528 kb on - strand, within gspC at 171.537 kb on - strand, within gspC at 171.538 kb on - strand, within gspC at 171.626 kb on + strand, within gspC at 171.634 kb on - strand, within gspC at 171.655 kb on - strand, within gspC at 171.663 kb on - strand, within gspC
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 168,874 + pckA SO0162 0.52 +0.8 168,874 + pckA SO0162 0.52 -0.9 168,876 + pckA SO0162 0.52 +1.5 168,882 - pckA SO0162 0.53 +0.1 168,918 - pckA SO0162 0.55 -1.8 168,977 + pckA SO0162 0.59 +0.3 168,985 - pckA SO0162 0.59 +0.9 168,985 - pckA SO0162 0.59 +0.8 169,001 + pckA SO0162 0.60 -1.9 169,008 + pckA SO0162 0.61 +1.3 169,013 - pckA SO0162 0.61 +0.8 169,174 - pckA SO0162 0.72 +0.6 169,200 + pckA SO0162 0.73 -1.5 169,222 + pckA SO0162 0.75 +0.1 169,247 + pckA SO0162 0.76 +0.5 169,267 - pckA SO0162 0.78 +0.3 169,337 - pckA SO0162 0.82 -0.8 169,352 + pckA SO0162 0.83 -1.4 169,409 + pckA SO0162 0.87 +1.0 169,481 + +0.6 169,487 + -1.9 169,564 + +0.5 169,567 + -0.4 169,572 + -1.1 169,586 - +0.8 169,623 + +0.5 169,628 + -1.7 169,742 + -0.4 169,742 + +0.8 169,780 + -1.0 169,780 + -1.6 169,785 + +1.1 169,848 - +0.6 169,912 - hslO SO0163 0.11 +5.6 169,945 - hslO SO0163 0.15 +5.0 169,972 + hslO SO0163 0.18 +4.8 169,987 + hslO SO0163 0.20 +4.5 170,009 + hslO SO0163 0.23 +5.0 170,060 + hslO SO0163 0.29 +4.3 170,060 + hslO SO0163 0.29 +4.0 170,060 + hslO SO0163 0.29 +4.4 170,060 + hslO SO0163 0.29 +4.8 170,060 + hslO SO0163 0.29 +4.6 170,060 + hslO SO0163 0.29 +4.6 170,068 - hslO SO0163 0.30 -4.5 170,068 - hslO SO0163 0.30 +5.0 170,068 - hslO SO0163 0.30 +5.6 170,068 - hslO SO0163 0.30 +4.4 170,068 - hslO SO0163 0.30 +5.0 170,068 - hslO SO0163 0.30 +2.3 170,077 + hslO SO0163 0.31 +3.9 170,085 - hslO SO0163 0.31 +3.5 170,123 + hslO SO0163 0.36 +4.1 170,188 - hslO SO0163 0.43 +4.9 170,213 + hslO SO0163 0.46 +4.8 170,241 - hslO SO0163 0.50 +4.2 170,242 + hslO SO0163 0.50 +4.0 170,250 - hslO SO0163 0.51 +5.0 170,250 - hslO SO0163 0.51 +5.4 170,338 + hslO SO0163 0.61 +5.0 170,369 + hslO SO0163 0.64 +4.8 170,377 - hslO SO0163 0.65 +4.7 170,549 + hslO SO0163 0.85 +4.5 170,678 - +3.0 170,772 - hslR SO0164 0.18 -1.0 170,984 - hslR SO0164 0.71 -1.2 170,984 - hslR SO0164 0.71 -1.6 171,080 + +2.7 171,088 - +0.4 171,139 + +3.9 171,163 + +0.7 171,171 + +1.6 171,204 - +0.5 171,281 + -1.1 171,305 - -3.3 171,329 + -0.8 171,361 + -2.2 171,387 - -3.2 171,453 - gspC SO0165 0.11 -1.4 171,513 - gspC SO0165 0.18 -0.7 171,518 - gspC SO0165 0.18 +1.5 171,520 + gspC SO0165 0.18 -3.3 171,528 - gspC SO0165 0.19 -2.1 171,537 - gspC SO0165 0.20 -2.1 171,538 - gspC SO0165 0.20 -1.6 171,626 + gspC SO0165 0.30 -1.5 171,634 - gspC SO0165 0.31 -0.7 171,655 - gspC SO0165 0.33 +0.2 171,663 - gspC SO0165 0.34 -2.3
Or see this region's nucleotide sequence