Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_78p00160 and PGA1_78p00170 are separated by 86 nucleotides PGA1_78p00170 and PGA1_78p00180 overlap by 8 nucleotides PGA1_78p00180 and PGA1_78p00190 are separated by 6 nucleotides
PGA1_78p00160: PGA1_78p00160 - putative sugar-binding periplasmic protein, at 19,025 to 20,254
_78p00160
PGA1_78p00170: PGA1_78p00170 - putative sn-glycerol-3-phosphate transport system permease protein UgpA, at 20,341 to 21,273
_78p00170
PGA1_78p00180: PGA1_78p00180 - putative sn-glycerol-3-phosphate transport system permease protein UgpE, at 21,266 to 22,195
_78p00180
PGA1_78p00190: PGA1_78p00190 - sn-glycerol-3-phosphate import ATP-binding protein UgbC, at 22,202 to 23,287
_78p00190
Position (kb) 20
21
22 Strain fitness (log2 ratio) -1
0
1
2 at 19.414 kb on + strand, within PGA1_78p00160 at 19.414 kb on + strand, within PGA1_78p00160 at 19.461 kb on - strand, within PGA1_78p00160 at 19.464 kb on + strand, within PGA1_78p00160 at 19.579 kb on - strand, within PGA1_78p00160 at 19.812 kb on - strand, within PGA1_78p00160 at 20.114 kb on + strand, within PGA1_78p00160 at 20.132 kb on - strand at 20.226 kb on + strand at 20.593 kb on - strand, within PGA1_78p00170 at 20.735 kb on + strand, within PGA1_78p00170 at 20.828 kb on + strand, within PGA1_78p00170 at 21.104 kb on - strand, within PGA1_78p00170 at 21.172 kb on - strand, within PGA1_78p00170 at 21.439 kb on + strand, within PGA1_78p00180 at 21.517 kb on + strand, within PGA1_78p00180 at 21.554 kb on + strand, within PGA1_78p00180 at 21.753 kb on + strand, within PGA1_78p00180 at 22.116 kb on + strand at 22.192 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 19,414 + PGA1_78p00160 0.32 +1.5 19,414 + PGA1_78p00160 0.32 +2.3 19,461 - PGA1_78p00160 0.35 +0.9 19,464 + PGA1_78p00160 0.36 +2.0 19,579 - PGA1_78p00160 0.45 -0.3 19,812 - PGA1_78p00160 0.64 +0.8 20,114 + PGA1_78p00160 0.89 +1.8 20,132 - -0.0 20,226 + +2.5 20,593 - PGA1_78p00170 0.27 +0.1 20,735 + PGA1_78p00170 0.42 -0.7 20,828 + PGA1_78p00170 0.52 -0.9 21,104 - PGA1_78p00170 0.82 +1.3 21,172 - PGA1_78p00170 0.89 +0.7 21,439 + PGA1_78p00180 0.19 -1.0 21,517 + PGA1_78p00180 0.27 +1.0 21,554 + PGA1_78p00180 0.31 -0.3 21,753 + PGA1_78p00180 0.52 -0.0 22,116 + +0.5 22,192 - +1.7
Or see this region's nucleotide sequence