Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purM and purN overlap by 1 nucleotides purN and SO2762 are separated by 140 nucleotides
SO2760: purM - phosphoribosylformylglycinamidine cyclo-ligase (NCBI ptt file), at 2,882,759 to 2,883,817
purM
SO2761: purN - phosphoribosylglycinamide formyltransferase (NCBI ptt file), at 2,883,817 to 2,884,461
purN
SO2762: SO2762 - nagD protein (NCBI ptt file), at 2,884,602 to 2,885,348
SO2762
Position (kb)
2883
2884
2885 Strain fitness (log2 ratio)
-2
-1
0
1 at 2882.854 kb on + strand at 2882.862 kb on - strand at 2882.883 kb on + strand, within purM at 2882.976 kb on + strand, within purM at 2883.151 kb on - strand, within purM at 2883.244 kb on - strand, within purM at 2883.244 kb on - strand, within purM at 2883.507 kb on - strand, within purM at 2883.666 kb on + strand, within purM at 2883.731 kb on - strand at 2883.962 kb on - strand, within purN at 2884.223 kb on + strand, within purN at 2884.357 kb on - strand, within purN at 2884.493 kb on - strand at 2884.583 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.645 kb on + strand at 2884.746 kb on + strand, within SO2762 at 2884.810 kb on - strand, within SO2762 at 2884.890 kb on - strand, within SO2762 at 2884.957 kb on + strand, within SO2762 at 2885.340 kb on - strand at 2885.342 kb on + strand at 2885.343 kb on + strand at 2885.361 kb on + strand at 2885.424 kb on - strand at 2885.424 kb on - strand at 2885.430 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,882,854 + +0.1 2,882,862 - +0.5 2,882,883 + purM SO2760 0.12 -0.0 2,882,976 + purM SO2760 0.20 -1.3 2,883,151 - purM SO2760 0.37 -0.8 2,883,244 - purM SO2760 0.46 +0.4 2,883,244 - purM SO2760 0.46 -0.9 2,883,507 - purM SO2760 0.71 +0.3 2,883,666 + purM SO2760 0.86 +1.4 2,883,731 - -0.7 2,883,962 - purN SO2761 0.22 -0.9 2,884,223 + purN SO2761 0.63 +0.2 2,884,357 - purN SO2761 0.84 -0.0 2,884,493 - -0.2 2,884,583 + -1.9 2,884,630 + -0.6 2,884,630 + -0.1 2,884,630 + +0.6 2,884,630 + -0.1 2,884,645 + -0.4 2,884,746 + SO2762 0.19 +0.1 2,884,810 - SO2762 0.28 +0.8 2,884,890 - SO2762 0.39 +0.6 2,884,957 + SO2762 0.48 -0.3 2,885,340 - -0.8 2,885,342 + -0.0 2,885,343 + -0.8 2,885,361 + +1.4 2,885,424 - -2.1 2,885,424 - +0.1 2,885,430 + -0.1
Or see this region's nucleotide sequence