Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0224

Experiment: Vanillin 2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0222 and Psest_0223 are separated by 146 nucleotidesPsest_0223 and Psest_0224 are separated by 123 nucleotidesPsest_0224 and Psest_0225 are separated by 87 nucleotides Psest_0222: Psest_0222 - phosphate ABC transporter, ATP-binding protein, at 230,844 to 231,671 _0222 Psest_0223: Psest_0223 - phosphate transport system regulatory protein PhoU, at 231,818 to 232,546 _0223 Psest_0224: Psest_0224 - Transcriptional regulator, at 232,670 to 233,584 _0224 Psest_0225: Psest_0225 - Predicted permease, DMT superfamily, at 233,672 to 234,583 _0225 Position (kb) 232 233 234Strain fitness (log2 ratio) -2 -1 0 1 2at 231.749 kb on + strandat 232.524 kb on + strandat 232.524 kb on + strandat 232.524 kb on + strandat 232.524 kb on + strandat 232.524 kb on + strandat 232.524 kb on + strandat 232.524 kb on + strandat 232.525 kb on - strandat 232.525 kb on - strandat 232.525 kb on - strandat 232.612 kb on + strandat 232.634 kb on + strandat 232.634 kb on + strandat 232.635 kb on - strandat 232.698 kb on + strandat 232.698 kb on + strandat 232.699 kb on - strandat 232.770 kb on + strand, within Psest_0224at 232.770 kb on + strand, within Psest_0224at 232.770 kb on + strand, within Psest_0224at 232.771 kb on - strand, within Psest_0224at 232.771 kb on - strand, within Psest_0224at 232.771 kb on - strand, within Psest_0224at 232.782 kb on + strand, within Psest_0224at 232.783 kb on - strand, within Psest_0224at 232.914 kb on + strand, within Psest_0224at 232.914 kb on + strand, within Psest_0224at 232.915 kb on - strand, within Psest_0224at 232.995 kb on + strand, within Psest_0224at 232.995 kb on + strand, within Psest_0224at 232.995 kb on + strand, within Psest_0224at 233.005 kb on + strand, within Psest_0224at 233.005 kb on + strand, within Psest_0224at 233.005 kb on + strand, within Psest_0224at 233.005 kb on + strand, within Psest_0224at 233.005 kb on + strand, within Psest_0224at 233.005 kb on + strand, within Psest_0224at 233.005 kb on + strand, within Psest_0224at 233.006 kb on - strand, within Psest_0224at 233.006 kb on - strand, within Psest_0224at 233.006 kb on - strand, within Psest_0224at 233.055 kb on + strand, within Psest_0224at 233.262 kb on + strand, within Psest_0224at 233.370 kb on + strand, within Psest_0224at 233.370 kb on + strand, within Psest_0224at 233.370 kb on + strand, within Psest_0224at 233.371 kb on - strand, within Psest_0224at 233.371 kb on - strand, within Psest_0224at 233.550 kb on + strandat 233.550 kb on + strandat 233.551 kb on - strandat 233.551 kb on - strandat 233.551 kb on - strandat 233.680 kb on + strandat 233.680 kb on + strandat 233.680 kb on + strandat 233.681 kb on - strandat 233.681 kb on - strandat 233.681 kb on - strandat 233.681 kb on - strandat 233.750 kb on + strandat 233.750 kb on + strandat 233.750 kb on + strandat 233.750 kb on + strandat 233.750 kb on + strandat 233.750 kb on + strandat 233.750 kb on + strandat 233.750 kb on + strandat 233.750 kb on + strandat 233.750 kb on + strandat 234.108 kb on + strand, within Psest_0225at 234.108 kb on + strand, within Psest_0225at 234.109 kb on - strand, within Psest_0225at 234.109 kb on - strand, within Psest_0225at 234.321 kb on - strand, within Psest_0225at 234.582 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vanillin 2 mM
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231,749 + +1.3
232,524 + -1.0
232,524 + -2.1
232,524 + +1.3
232,524 + +1.0
232,524 + +0.3
232,524 + -0.4
232,524 + +0.3
232,525 - -0.7
232,525 - +1.3
232,525 - +0.3
232,612 + -0.6
232,634 + +1.3
232,634 + +1.3
232,635 - -0.8
232,698 + +0.3
232,698 + +0.3
232,699 - +0.3
232,770 + Psest_0224 0.11 +2.3
232,770 + Psest_0224 0.11 +0.3
232,770 + Psest_0224 0.11 +0.6
232,771 - Psest_0224 0.11 -1.0
232,771 - Psest_0224 0.11 -0.4
232,771 - Psest_0224 0.11 +1.3
232,782 + Psest_0224 0.12 -0.3
232,783 - Psest_0224 0.12 -0.4
232,914 + Psest_0224 0.27 +1.1
232,914 + Psest_0224 0.27 +0.3
232,915 - Psest_0224 0.27 -1.0
232,995 + Psest_0224 0.36 -1.0
232,995 + Psest_0224 0.36 -0.5
232,995 + Psest_0224 0.36 +1.3
233,005 + Psest_0224 0.37 +0.7
233,005 + Psest_0224 0.37 -0.7
233,005 + Psest_0224 0.37 -2.0
233,005 + Psest_0224 0.37 +1.3
233,005 + Psest_0224 0.37 +1.3
233,005 + Psest_0224 0.37 -0.3
233,005 + Psest_0224 0.37 +0.3
233,006 - Psest_0224 0.37 +0.2
233,006 - Psest_0224 0.37 +0.3
233,006 - Psest_0224 0.37 -0.8
233,055 + Psest_0224 0.42 +2.3
233,262 + Psest_0224 0.65 -1.7
233,370 + Psest_0224 0.77 -0.3
233,370 + Psest_0224 0.77 +0.1
233,370 + Psest_0224 0.77 -0.0
233,371 - Psest_0224 0.77 +0.9
233,371 - Psest_0224 0.77 -1.1
233,550 + -0.3
233,550 + -1.0
233,551 - +1.3
233,551 - +1.3
233,551 - +2.3
233,680 + +1.3
233,680 + +1.0
233,680 + +1.3
233,681 - +2.3
233,681 - -0.3
233,681 - +2.3
233,681 - +1.3
233,750 + +2.3
233,750 + +1.3
233,750 + +0.3
233,750 + -1.7
233,750 + -0.7
233,750 + +1.3
233,750 + +0.3
233,750 + -1.1
233,750 + -1.3
233,750 + +1.3
234,108 + Psest_0225 0.48 +0.3
234,108 + Psest_0225 0.48 +0.6
234,109 - Psest_0225 0.48 -0.3
234,109 - Psest_0225 0.48 +0.7
234,321 - Psest_0225 0.71 -0.7
234,582 - -1.3

Or see this region's nucleotide sequence