Experiment: syringaldehyde 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purM and purN overlap by 1 nucleotides purN and SO2762 are separated by 140 nucleotides SO2762 and SO2763 are separated by 316 nucleotides
SO2760: purM - phosphoribosylformylglycinamidine cyclo-ligase (NCBI ptt file), at 2,882,759 to 2,883,817
purM
SO2761: purN - phosphoribosylglycinamide formyltransferase (NCBI ptt file), at 2,883,817 to 2,884,461
purN
SO2762: SO2762 - nagD protein (NCBI ptt file), at 2,884,602 to 2,885,348
SO2762
SO2763: SO2763 - conserved hypothetical protein (NCBI ptt file), at 2,885,665 to 2,886,480
SO2763
Position (kb)
2884
2885
2886 Strain fitness (log2 ratio)
-1
0
1
2 at 2883.666 kb on + strand, within purM at 2883.731 kb on - strand at 2883.962 kb on - strand, within purN at 2884.223 kb on + strand, within purN at 2884.357 kb on - strand, within purN at 2884.493 kb on - strand at 2884.583 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.645 kb on + strand at 2884.746 kb on + strand, within SO2762 at 2884.810 kb on - strand, within SO2762 at 2884.890 kb on - strand, within SO2762 at 2884.957 kb on + strand, within SO2762 at 2885.340 kb on - strand at 2885.342 kb on + strand at 2885.343 kb on + strand at 2885.361 kb on + strand at 2885.424 kb on - strand at 2885.424 kb on - strand at 2885.430 kb on + strand at 2885.769 kb on + strand, within SO2763 at 2885.875 kb on - strand, within SO2763 at 2885.983 kb on + strand, within SO2763 at 2886.138 kb on + strand, within SO2763 at 2886.146 kb on - strand, within SO2763 at 2886.147 kb on + strand, within SO2763 at 2886.224 kb on - strand, within SO2763 at 2886.246 kb on + strand, within SO2763 at 2886.246 kb on + strand, within SO2763 at 2886.246 kb on + strand, within SO2763 at 2886.246 kb on + strand, within SO2763 at 2886.254 kb on - strand, within SO2763 at 2886.254 kb on - strand, within SO2763 at 2886.254 kb on - strand, within SO2763 at 2886.281 kb on - strand, within SO2763 at 2886.281 kb on - strand, within SO2763
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 1 mM remove 2,883,666 + purM SO2760 0.86 +2.0 2,883,731 - -1.4 2,883,962 - purN SO2761 0.22 +0.3 2,884,223 + purN SO2761 0.63 -0.6 2,884,357 - purN SO2761 0.84 -0.3 2,884,493 - -0.4 2,884,583 + -0.5 2,884,630 + +0.2 2,884,630 + +0.3 2,884,630 + +0.5 2,884,630 + -0.0 2,884,645 + +0.6 2,884,746 + SO2762 0.19 -0.5 2,884,810 - SO2762 0.28 +0.5 2,884,890 - SO2762 0.39 -0.8 2,884,957 + SO2762 0.48 -0.7 2,885,340 - +0.2 2,885,342 + +0.3 2,885,343 + +1.7 2,885,361 + +0.6 2,885,424 - -0.2 2,885,424 - -0.2 2,885,430 + -0.7 2,885,769 + SO2763 0.13 -0.4 2,885,875 - SO2763 0.26 -0.2 2,885,983 + SO2763 0.39 +0.2 2,886,138 + SO2763 0.58 -0.7 2,886,146 - SO2763 0.59 -0.3 2,886,147 + SO2763 0.59 +2.0 2,886,224 - SO2763 0.69 -0.9 2,886,246 + SO2763 0.71 +0.1 2,886,246 + SO2763 0.71 +0.2 2,886,246 + SO2763 0.71 -0.2 2,886,246 + SO2763 0.71 -0.7 2,886,254 - SO2763 0.72 +1.0 2,886,254 - SO2763 0.72 +0.2 2,886,254 - SO2763 0.72 +0.1 2,886,281 - SO2763 0.75 -1.0 2,886,281 - SO2763 0.75 -1.1
Or see this region's nucleotide sequence