Experiment: Ying_AminoAcid20 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS13160 and ABIE51_RS13165 are separated by 195 nucleotides ABIE51_RS13165 and ABIE51_RS13170 overlap by 4 nucleotides ABIE51_RS13170 and ABIE51_RS13175 are separated by 97 nucleotides ABIE51_RS13175 and ABIE51_RS13180 are separated by 2 nucleotides
ABIE51_RS13160: ABIE51_RS13160 - carboxyl transferase domain-containing protein, at 2,841,315 to 2,842,940
_RS13160
ABIE51_RS13165: ABIE51_RS13165 - polymer-forming cytoskeletal protein, at 2,843,136 to 2,843,738
_RS13165
ABIE51_RS13170: ABIE51_RS13170 - hypothetical protein, at 2,843,735 to 2,844,097
_RS13170
ABIE51_RS13175: ABIE51_RS13175 - DUF6587 family protein, at 2,844,195 to 2,844,449
_RS13175
ABIE51_RS13180: ABIE51_RS13180 - ferrous iron transport protein B, at 2,844,452 to 2,846,308
_RS13180
Position (kb)
2843
2844
2845 Strain fitness (log2 ratio)
-1
0
1 at 2842.768 kb on - strand, within ABIE51_RS13160 at 2842.788 kb on + strand at 2842.788 kb on + strand at 2842.789 kb on - strand at 2842.789 kb on - strand at 2842.789 kb on - strand at 2842.791 kb on + strand at 2842.792 kb on - strand at 2842.792 kb on - strand at 2842.995 kb on - strand at 2842.995 kb on - strand at 2843.942 kb on + strand, within ABIE51_RS13170 at 2843.943 kb on - strand, within ABIE51_RS13170
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2 remove 2,842,768 - ABIE51_RS13160 0.89 -0.4 2,842,788 + -0.6 2,842,788 + +0.7 2,842,789 - -1.0 2,842,789 - -1.1 2,842,789 - -0.8 2,842,791 + -0.8 2,842,792 - -0.8 2,842,792 - +0.4 2,842,995 - +0.5 2,842,995 - +0.1 2,843,942 + ABIE51_RS13170 0.57 -0.1 2,843,943 - ABIE51_RS13170 0.57 +1.2
Or see this region's nucleotide sequence