Strain Fitness in Lysobacter sp. OAE881 around ABIE51_RS16100

Experiment: Ying_AminoAcid20 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABIE51_RS16090 and ABIE51_RS16095 are separated by 22 nucleotidesABIE51_RS16095 and ABIE51_RS16100 are separated by 113 nucleotidesABIE51_RS16100 and ABIE51_RS16105 are separated by 37 nucleotidesABIE51_RS16105 and ABIE51_RS16110 are separated by 129 nucleotides ABIE51_RS16090: ABIE51_RS16090 - kinase, at 3,484,688 to 3,485,494 _RS16090 ABIE51_RS16095: ABIE51_RS16095 - shikimate kinase, at 3,485,517 to 3,486,041 _RS16095 ABIE51_RS16100: ABIE51_RS16100 - 3-dehydroquinate synthase, at 3,486,155 to 3,487,261 _RS16100 ABIE51_RS16105: ABIE51_RS16105 - WGR domain-containing protein, at 3,487,299 to 3,487,553 _RS16105 ABIE51_RS16110: ABIE51_RS16110 - uroporphyrinogen decarboxylase, at 3,487,683 to 3,488,753 _RS16110 Position (kb) 3486 3487 3488Strain fitness (log2 ratio) -1 0 1at 3485.382 kb on + strand, within ABIE51_RS16090at 3485.504 kb on + strandat 3485.505 kb on - strandat 3485.505 kb on - strandat 3485.943 kb on + strand, within ABIE51_RS16095at 3485.943 kb on + strand, within ABIE51_RS16095at 3486.197 kb on + strandat 3487.272 kb on + strandat 3487.272 kb on + strandat 3487.273 kb on - strandat 3487.287 kb on + strandat 3487.287 kb on + strandat 3487.288 kb on - strandat 3487.551 kb on + strandat 3487.613 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2
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3,485,382 + ABIE51_RS16090 0.86 +0.2
3,485,504 + +0.1
3,485,505 - -0.0
3,485,505 - -1.4
3,485,943 + ABIE51_RS16095 0.81 +0.5
3,485,943 + ABIE51_RS16095 0.81 -0.4
3,486,197 + +1.4
3,487,272 + +0.2
3,487,272 + +0.2
3,487,273 - -0.2
3,487,287 + +0.6
3,487,287 + -0.7
3,487,288 - +1.6
3,487,551 + +0.2
3,487,613 - +0.8

Or see this region's nucleotide sequence