Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS16090 and ABIE51_RS16095 are separated by 22 nucleotides ABIE51_RS16095 and ABIE51_RS16100 are separated by 113 nucleotides ABIE51_RS16100 and ABIE51_RS16105 are separated by 37 nucleotides ABIE51_RS16105 and ABIE51_RS16110 are separated by 129 nucleotides
ABIE51_RS16090: ABIE51_RS16090 - kinase, at 3,484,688 to 3,485,494
_RS16090
ABIE51_RS16095: ABIE51_RS16095 - shikimate kinase, at 3,485,517 to 3,486,041
_RS16095
ABIE51_RS16100: ABIE51_RS16100 - 3-dehydroquinate synthase, at 3,486,155 to 3,487,261
_RS16100
ABIE51_RS16105: ABIE51_RS16105 - WGR domain-containing protein, at 3,487,299 to 3,487,553
_RS16105
ABIE51_RS16110: ABIE51_RS16110 - uroporphyrinogen decarboxylase, at 3,487,683 to 3,488,753
_RS16110
Position (kb)
3486
3487
3488 Strain fitness (log2 ratio)
-1
0
1 at 3485.382 kb on + strand, within ABIE51_RS16090 at 3485.504 kb on + strand at 3485.505 kb on - strand at 3485.505 kb on - strand at 3485.943 kb on + strand, within ABIE51_RS16095 at 3485.943 kb on + strand, within ABIE51_RS16095 at 3486.197 kb on + strand at 3487.272 kb on + strand at 3487.272 kb on + strand at 3487.273 kb on - strand at 3487.287 kb on + strand at 3487.287 kb on + strand at 3487.288 kb on - strand at 3487.551 kb on + strand at 3487.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 3,485,382 + ABIE51_RS16090 0.86 +0.2 3,485,504 + +0.1 3,485,505 - -0.0 3,485,505 - -1.4 3,485,943 + ABIE51_RS16095 0.81 +0.5 3,485,943 + ABIE51_RS16095 0.81 -0.4 3,486,197 + +1.4 3,487,272 + +0.2 3,487,272 + +0.2 3,487,273 - -0.2 3,487,287 + +0.6 3,487,287 + -0.7 3,487,288 - +1.6 3,487,551 + +0.2 3,487,613 - +0.8
Or see this region's nucleotide sequence