Experiment: 7H9 with Clarithromycin 0.967 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0299 and Rv0300 are separated by 47 nucleotides Rv0300 and Rv0301 overlap by 4 nucleotides Rv0301 and Rv0302 are separated by 135 nucleotides Rv0302 and Rv0303 overlap by 4 nucleotides Rv0303 and Rv0304c are separated by 7 nucleotides
Rv0299: Rv0299 - Hypothetical protein, at 363,476 to 363,778
Rv0299
Rv0300: Rv0300 - Possible antitoxin VapB2, at 363,826 to 364,047
Rv0300
Rv0301: Rv0301 - Possible toxin VapC2, at 364,044 to 364,469
Rv0301
Rv0302: Rv0302 - Probable transcriptional regulatory protein (probably TetR/AcrR-family), at 364,605 to 365,237
Rv0302
Rv0303: Rv0303 - Probable dehydrogenase/reductase, at 365,234 to 366,142
Rv0303
Rv0304c: Rv0304c - PPE family protein PPE5, at 366,150 to 372,764
Rv0304c
Position (kb) 364
365
366 Strain fitness (log2 ratio) -1
0
1 at 363.814 kb on + strand at 363.815 kb on - strand at 363.836 kb on + strand at 363.837 kb on - strand at 363.837 kb on - strand at 363.866 kb on + strand, within Rv0300 at 363.866 kb on + strand, within Rv0300 at 363.867 kb on - strand, within Rv0300 at 364.027 kb on - strand at 364.144 kb on + strand, within Rv0301 at 364.145 kb on - strand, within Rv0301 at 364.145 kb on - strand, within Rv0301 at 364.145 kb on - strand, within Rv0301 at 364.174 kb on + strand, within Rv0301 at 364.175 kb on - strand, within Rv0301 at 364.175 kb on - strand, within Rv0301 at 364.251 kb on + strand, within Rv0301 at 364.251 kb on + strand, within Rv0301 at 364.252 kb on - strand, within Rv0301 at 364.252 kb on - strand, within Rv0301 at 364.252 kb on - strand, within Rv0301 at 364.256 kb on - strand, within Rv0301 at 364.256 kb on - strand, within Rv0301 at 364.256 kb on - strand, within Rv0301 at 364.381 kb on + strand, within Rv0301 at 364.382 kb on - strand, within Rv0301 at 364.382 kb on - strand, within Rv0301 at 364.568 kb on - strand at 364.741 kb on + strand, within Rv0302 at 364.755 kb on - strand, within Rv0302 at 364.755 kb on - strand, within Rv0302 at 364.758 kb on + strand, within Rv0302 at 364.942 kb on - strand, within Rv0302 at 365.131 kb on - strand, within Rv0302 at 365.178 kb on + strand at 365.179 kb on - strand at 365.179 kb on - strand at 365.304 kb on + strand at 365.565 kb on + strand, within Rv0303 at 365.693 kb on + strand, within Rv0303 at 365.695 kb on + strand, within Rv0303 at 365.696 kb on - strand, within Rv0303 at 365.696 kb on - strand, within Rv0303 at 365.696 kb on - strand, within Rv0303 at 365.762 kb on + strand, within Rv0303 at 365.762 kb on + strand, within Rv0303 at 365.798 kb on + strand, within Rv0303 at 365.799 kb on - strand, within Rv0303 at 365.865 kb on + strand, within Rv0303 at 365.918 kb on + strand, within Rv0303 at 365.918 kb on + strand, within Rv0303 at 366.077 kb on + strand at 366.140 kb on + strand at 366.140 kb on + strand at 366.140 kb on + strand at 366.141 kb on - strand at 366.141 kb on - strand at 366.141 kb on - strand at 366.141 kb on - strand at 366.159 kb on - strand at 366.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Clarithromycin 0.967 ug/mL remove 363,814 + +1.0 363,815 - +0.4 363,836 + -0.8 363,837 - -0.1 363,837 - -0.3 363,866 + Rv0300 0.18 -1.7 363,866 + Rv0300 0.18 -0.5 363,867 - Rv0300 0.18 -0.4 364,027 - -1.6 364,144 + Rv0301 0.23 +0.2 364,145 - Rv0301 0.24 -0.1 364,145 - Rv0301 0.24 +0.1 364,145 - Rv0301 0.24 -0.2 364,174 + Rv0301 0.31 +0.2 364,175 - Rv0301 0.31 -0.3 364,175 - Rv0301 0.31 +0.3 364,251 + Rv0301 0.49 +0.4 364,251 + Rv0301 0.49 -0.7 364,252 - Rv0301 0.49 +1.5 364,252 - Rv0301 0.49 +0.2 364,252 - Rv0301 0.49 +0.3 364,256 - Rv0301 0.50 +0.4 364,256 - Rv0301 0.50 -0.4 364,256 - Rv0301 0.50 -0.3 364,381 + Rv0301 0.79 -0.4 364,382 - Rv0301 0.79 -0.1 364,382 - Rv0301 0.79 -0.2 364,568 - +0.0 364,741 + Rv0302 0.21 +0.4 364,755 - Rv0302 0.24 +0.9 364,755 - Rv0302 0.24 +0.1 364,758 + Rv0302 0.24 +0.1 364,942 - Rv0302 0.53 +1.5 365,131 - Rv0302 0.83 -0.3 365,178 + -0.2 365,179 - -0.3 365,179 - -0.0 365,304 + +0.1 365,565 + Rv0303 0.36 +0.5 365,693 + Rv0303 0.50 -0.8 365,695 + Rv0303 0.51 -0.5 365,696 - Rv0303 0.51 -0.3 365,696 - Rv0303 0.51 -0.5 365,696 - Rv0303 0.51 -0.6 365,762 + Rv0303 0.58 -0.1 365,762 + Rv0303 0.58 -0.3 365,798 + Rv0303 0.62 -0.4 365,799 - Rv0303 0.62 -1.0 365,865 + Rv0303 0.69 +0.2 365,918 + Rv0303 0.75 -0.3 365,918 + Rv0303 0.75 -0.6 366,077 + -0.1 366,140 + -0.0 366,140 + -0.1 366,140 + -0.5 366,141 - +0.4 366,141 - -0.5 366,141 - -0.3 366,141 - +1.1 366,159 - +0.3 366,189 - +0.2
Or see this region's nucleotide sequence