Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2063A

Experiment: 7H9 with Delamanid 0.236 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2062c and Rv2063 are separated by 77 nucleotidesRv2063 and Rv2063A overlap by 8 nucleotidesRv2063A and Rv2064 overlap by 17 nucleotides Rv2062c: Rv2062c - Cobalamin biosynthesis protein CobN, at 2,317,169 to 2,320,753 Rv2062c Rv2063: Rv2063 - Antitoxin MazE7, at 2,320,831 to 2,321,064 Rv2063 Rv2063A: Rv2063A - Possible toxin MazF7, at 2,321,057 to 2,321,467 Rv2063A Rv2064: Rv2064 - Precorrin-3B synthase CobG, at 2,321,451 to 2,322,542 Rv2064 Position (kb) 2321 2322Strain fitness (log2 ratio) -2 -1 0 1at 2320.279 kb on - strand, within Rv2062cat 2320.443 kb on + strandat 2320.443 kb on + strandat 2320.444 kb on - strandat 2320.444 kb on - strandat 2320.444 kb on - strandat 2320.824 kb on + strandat 2321.163 kb on + strand, within Rv2063Aat 2321.163 kb on + strand, within Rv2063Aat 2321.263 kb on - strand, within Rv2063Aat 2321.729 kb on + strand, within Rv2064at 2322.041 kb on - strand, within Rv2064

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Delamanid 0.236 ug/mL
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2,320,279 - Rv2062c 0.87 -0.6
2,320,443 + -0.4
2,320,443 + +0.4
2,320,444 - -0.5
2,320,444 - -0.1
2,320,444 - +0.8
2,320,824 + -0.6
2,321,163 + Rv2063A 0.26 -2.5
2,321,163 + Rv2063A 0.26 -0.5
2,321,263 - Rv2063A 0.50 +0.2
2,321,729 + Rv2064 0.25 -0.4
2,322,041 - Rv2064 0.54 +0.4

Or see this region's nucleotide sequence