Experiment: NL-CCM; vial
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22835 and EX31_RS22840 are separated by 185 nucleotides EX31_RS22840 and EX31_RS22845 are separated by 146 nucleotides EX31_RS22845 and EX31_RS22850 are separated by 102 nucleotides
EX31_RS22835: EX31_RS22835 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD, at 327,544 to 328,416
_RS22835
EX31_RS22840: EX31_RS22840 - L-lactate dehydrogenase, at 328,602 to 329,513
_RS22840
EX31_RS22845: EX31_RS22845 - tRNA-Arg, at 329,660 to 329,736
_RS22845
EX31_RS22850: EX31_RS22850 - DUF4942 domain-containing protein, at 329,839 to 330,690
_RS22850
Position (kb)
328
329
330 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 328.434 kb on - strand at 328.438 kb on - strand at 328.438 kb on - strand at 328.438 kb on - strand at 328.505 kb on - strand at 328.512 kb on + strand at 328.559 kb on + strand at 328.559 kb on + strand at 328.560 kb on - strand at 328.560 kb on - strand at 328.564 kb on + strand at 328.568 kb on + strand at 328.568 kb on + strand at 328.581 kb on - strand at 328.715 kb on + strand, within EX31_RS22840 at 328.715 kb on + strand, within EX31_RS22840 at 328.715 kb on + strand, within EX31_RS22840 at 328.715 kb on + strand, within EX31_RS22840 at 328.716 kb on - strand, within EX31_RS22840 at 328.716 kb on - strand, within EX31_RS22840 at 328.716 kb on - strand, within EX31_RS22840 at 328.795 kb on - strand, within EX31_RS22840 at 329.291 kb on + strand, within EX31_RS22840 at 329.379 kb on + strand, within EX31_RS22840 at 329.379 kb on + strand, within EX31_RS22840 at 329.380 kb on - strand, within EX31_RS22840 at 329.380 kb on - strand, within EX31_RS22840 at 329.380 kb on - strand, within EX31_RS22840 at 329.380 kb on - strand, within EX31_RS22840 at 329.414 kb on + strand, within EX31_RS22840 at 329.415 kb on - strand, within EX31_RS22840 at 329.419 kb on + strand, within EX31_RS22840 at 329.420 kb on - strand, within EX31_RS22840 at 329.420 kb on - strand, within EX31_RS22840 at 329.420 kb on - strand, within EX31_RS22840 at 329.429 kb on - strand at 329.429 kb on - strand at 329.459 kb on - strand at 329.459 kb on - strand at 329.511 kb on + strand at 329.512 kb on - strand at 329.529 kb on + strand at 329.529 kb on + strand at 329.529 kb on + strand at 329.529 kb on + strand at 329.529 kb on + strand at 329.529 kb on + strand at 329.529 kb on + strand at 329.529 kb on + strand at 329.529 kb on + strand at 329.530 kb on - strand at 329.530 kb on - strand at 329.530 kb on - strand at 329.530 kb on - strand at 329.554 kb on - strand at 329.554 kb on - strand at 329.575 kb on + strand at 329.794 kb on + strand at 329.810 kb on + strand at 329.810 kb on + strand at 329.810 kb on + strand at 329.811 kb on - strand at 329.850 kb on + strand at 329.850 kb on + strand at 329.883 kb on + strand at 329.883 kb on + strand at 329.883 kb on + strand at 329.884 kb on - strand at 329.884 kb on - strand at 329.948 kb on + strand, within EX31_RS22850 at 329.995 kb on - strand, within EX31_RS22850 at 329.995 kb on - strand, within EX31_RS22850 at 330.049 kb on - strand, within EX31_RS22850 at 330.049 kb on - strand, within EX31_RS22850 at 330.123 kb on + strand, within EX31_RS22850 at 330.124 kb on - strand, within EX31_RS22850 at 330.137 kb on + strand, within EX31_RS22850 at 330.154 kb on - strand, within EX31_RS22850 at 330.158 kb on - strand, within EX31_RS22850 at 330.158 kb on - strand, within EX31_RS22850 at 330.158 kb on - strand, within EX31_RS22850 at 330.163 kb on - strand, within EX31_RS22850 at 330.211 kb on + strand, within EX31_RS22850 at 330.211 kb on + strand, within EX31_RS22850 at 330.211 kb on + strand, within EX31_RS22850 at 330.220 kb on - strand, within EX31_RS22850 at 330.231 kb on + strand, within EX31_RS22850 at 330.232 kb on - strand, within EX31_RS22850 at 330.232 kb on - strand, within EX31_RS22850 at 330.282 kb on + strand, within EX31_RS22850 at 330.283 kb on - strand, within EX31_RS22850 at 330.435 kb on + strand, within EX31_RS22850
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; vial remove 328,434 - +1.3 328,438 - -0.5 328,438 - -0.0 328,438 - +0.3 328,505 - +0.6 328,512 + -1.5 328,559 + -0.5 328,559 + -0.5 328,560 - -0.8 328,560 - +0.7 328,564 + +0.7 328,568 + +1.0 328,568 + -1.3 328,581 - -0.1 328,715 + EX31_RS22840 0.12 -0.8 328,715 + EX31_RS22840 0.12 -1.0 328,715 + EX31_RS22840 0.12 -1.1 328,715 + EX31_RS22840 0.12 -0.2 328,716 - EX31_RS22840 0.12 +0.3 328,716 - EX31_RS22840 0.12 -0.9 328,716 - EX31_RS22840 0.12 -0.1 328,795 - EX31_RS22840 0.21 +0.8 329,291 + EX31_RS22840 0.76 -0.3 329,379 + EX31_RS22840 0.85 -0.6 329,379 + EX31_RS22840 0.85 +0.8 329,380 - EX31_RS22840 0.85 -1.1 329,380 - EX31_RS22840 0.85 -1.1 329,380 - EX31_RS22840 0.85 +0.3 329,380 - EX31_RS22840 0.85 -2.2 329,414 + EX31_RS22840 0.89 -1.9 329,415 - EX31_RS22840 0.89 -0.2 329,419 + EX31_RS22840 0.90 +0.1 329,420 - EX31_RS22840 0.90 -1.1 329,420 - EX31_RS22840 0.90 +0.3 329,420 - EX31_RS22840 0.90 +0.7 329,429 - +2.1 329,429 - +1.5 329,459 - +0.1 329,459 - +0.1 329,511 + +0.5 329,512 - -0.7 329,529 + +2.9 329,529 + -0.7 329,529 + +0.3 329,529 + -0.4 329,529 + +0.6 329,529 + -0.6 329,529 + +0.1 329,529 + +0.4 329,529 + +0.0 329,530 - +0.2 329,530 - +0.9 329,530 - +0.1 329,530 - -0.3 329,554 - -0.7 329,554 - -0.4 329,575 + +1.0 329,794 + -1.9 329,810 + +0.9 329,810 + -0.1 329,810 + +0.1 329,811 - +1.3 329,850 + -0.2 329,850 + +0.1 329,883 + -2.2 329,883 + +0.2 329,883 + +0.1 329,884 - +0.2 329,884 - -0.1 329,948 + EX31_RS22850 0.13 -1.3 329,995 - EX31_RS22850 0.18 +0.6 329,995 - EX31_RS22850 0.18 +0.1 330,049 - EX31_RS22850 0.25 -0.6 330,049 - EX31_RS22850 0.25 +0.6 330,123 + EX31_RS22850 0.33 -1.5 330,124 - EX31_RS22850 0.33 -0.3 330,137 + EX31_RS22850 0.35 -0.3 330,154 - EX31_RS22850 0.37 +0.3 330,158 - EX31_RS22850 0.37 -0.5 330,158 - EX31_RS22850 0.37 -0.7 330,158 - EX31_RS22850 0.37 -0.2 330,163 - EX31_RS22850 0.38 -0.6 330,211 + EX31_RS22850 0.44 +0.5 330,211 + EX31_RS22850 0.44 +0.3 330,211 + EX31_RS22850 0.44 -1.4 330,220 - EX31_RS22850 0.45 -0.1 330,231 + EX31_RS22850 0.46 -0.6 330,232 - EX31_RS22850 0.46 +0.1 330,232 - EX31_RS22850 0.46 -0.6 330,282 + EX31_RS22850 0.52 -1.1 330,283 - EX31_RS22850 0.52 -0.2 330,435 + EX31_RS22850 0.70 +0.6
Or see this region's nucleotide sequence