Experiment: NL-CCM; vial
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21915 and EX31_RS21920 are separated by 12 nucleotides EX31_RS21920 and EX31_RS21925 are separated by 46 nucleotides EX31_RS21925 and EX31_RS21930 are separated by 34 nucleotides EX31_RS21930 and EX31_RS21935 are separated by 137 nucleotides
EX31_RS21915: EX31_RS21915 - fatty acid desaturase, at 128,237 to 129,244
_RS21915
EX31_RS21920: EX31_RS21920 - PhnD/SsuA/transferrin family substrate-binding protein, at 129,257 to 130,063
_RS21920
EX31_RS21925: EX31_RS21925 - 3-mercaptopyruvate sulfurtransferase, at 130,110 to 130,955
_RS21925
EX31_RS21930: EX31_RS21930 - GNAT family N-acetyltransferase, at 130,990 to 131,538
_RS21930
EX31_RS21935: EX31_RS21935 - helix-turn-helix domain-containing protein, at 131,676 to 132,467
_RS21935
Position (kb) 130
131 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 129.219 kb on - strand at 129.225 kb on - strand at 129.281 kb on + strand at 129.282 kb on - strand at 129.373 kb on + strand, within EX31_RS21920 at 129.373 kb on + strand, within EX31_RS21920 at 129.374 kb on - strand, within EX31_RS21920 at 129.390 kb on + strand, within EX31_RS21920 at 129.418 kb on - strand, within EX31_RS21920 at 129.491 kb on + strand, within EX31_RS21920 at 129.491 kb on + strand, within EX31_RS21920 at 129.599 kb on - strand, within EX31_RS21920 at 129.623 kb on + strand, within EX31_RS21920 at 129.623 kb on + strand, within EX31_RS21920 at 129.624 kb on - strand, within EX31_RS21920 at 129.625 kb on + strand, within EX31_RS21920 at 129.625 kb on + strand, within EX31_RS21920 at 129.625 kb on + strand, within EX31_RS21920 at 129.626 kb on - strand, within EX31_RS21920 at 129.626 kb on - strand, within EX31_RS21920 at 129.720 kb on - strand, within EX31_RS21920 at 129.856 kb on + strand, within EX31_RS21920 at 129.856 kb on + strand, within EX31_RS21920 at 129.856 kb on + strand, within EX31_RS21920 at 129.856 kb on + strand, within EX31_RS21920 at 129.856 kb on + strand, within EX31_RS21920 at 129.856 kb on + strand, within EX31_RS21920 at 129.856 kb on + strand, within EX31_RS21920 at 129.856 kb on + strand, within EX31_RS21920 at 129.856 kb on + strand, within EX31_RS21920 at 129.857 kb on - strand, within EX31_RS21920 at 129.857 kb on - strand, within EX31_RS21920 at 129.857 kb on - strand, within EX31_RS21920 at 129.857 kb on - strand, within EX31_RS21920 at 129.857 kb on - strand, within EX31_RS21920 at 129.857 kb on - strand, within EX31_RS21920 at 129.906 kb on + strand, within EX31_RS21920 at 129.907 kb on - strand, within EX31_RS21920 at 129.907 kb on - strand, within EX31_RS21920 at 129.942 kb on + strand, within EX31_RS21920 at 129.942 kb on + strand, within EX31_RS21920 at 129.942 kb on + strand, within EX31_RS21920 at 129.942 kb on + strand, within EX31_RS21920 at 129.943 kb on - strand, within EX31_RS21920 at 129.946 kb on + strand, within EX31_RS21920 at 129.946 kb on + strand, within EX31_RS21920 at 129.946 kb on + strand, within EX31_RS21920 at 129.946 kb on + strand, within EX31_RS21920 at 129.947 kb on - strand, within EX31_RS21920 at 129.947 kb on - strand, within EX31_RS21920 at 129.947 kb on - strand, within EX31_RS21920 at 129.958 kb on + strand, within EX31_RS21920 at 129.959 kb on - strand, within EX31_RS21920 at 129.981 kb on - strand, within EX31_RS21920 at 130.008 kb on + strand at 130.008 kb on + strand at 130.008 kb on + strand at 130.009 kb on - strand at 130.142 kb on + strand at 130.171 kb on + strand at 130.188 kb on - strand at 130.560 kb on - strand, within EX31_RS21925 at 130.560 kb on - strand, within EX31_RS21925 at 130.560 kb on - strand, within EX31_RS21925 at 130.602 kb on + strand, within EX31_RS21925 at 130.602 kb on + strand, within EX31_RS21925 at 130.603 kb on - strand, within EX31_RS21925 at 130.657 kb on - strand, within EX31_RS21925 at 130.672 kb on + strand, within EX31_RS21925 at 130.673 kb on - strand, within EX31_RS21925 at 130.673 kb on - strand, within EX31_RS21925 at 130.795 kb on + strand, within EX31_RS21925 at 130.796 kb on - strand, within EX31_RS21925 at 130.796 kb on - strand, within EX31_RS21925 at 130.796 kb on - strand, within EX31_RS21925 at 130.796 kb on - strand, within EX31_RS21925 at 130.826 kb on - strand, within EX31_RS21925 at 130.853 kb on + strand, within EX31_RS21925 at 130.871 kb on + strand at 130.928 kb on + strand at 130.928 kb on + strand at 130.928 kb on + strand at 130.928 kb on + strand at 130.929 kb on - strand at 130.932 kb on + strand at 130.932 kb on + strand at 130.932 kb on + strand at 130.932 kb on + strand at 130.933 kb on - strand at 130.933 kb on - strand at 130.933 kb on - strand at 131.015 kb on + strand at 131.015 kb on + strand at 131.016 kb on - strand at 131.016 kb on - strand at 131.016 kb on - strand at 131.016 kb on - strand at 131.016 kb on - strand at 131.046 kb on - strand, within EX31_RS21930 at 131.048 kb on + strand, within EX31_RS21930 at 131.049 kb on - strand, within EX31_RS21930 at 131.049 kb on - strand, within EX31_RS21930 at 131.218 kb on - strand, within EX31_RS21930 at 131.220 kb on - strand, within EX31_RS21930 at 131.257 kb on - strand, within EX31_RS21930 at 131.257 kb on - strand, within EX31_RS21930 at 131.258 kb on + strand, within EX31_RS21930 at 131.320 kb on + strand, within EX31_RS21930 at 131.321 kb on - strand, within EX31_RS21930 at 131.321 kb on - strand, within EX31_RS21930 at 131.321 kb on - strand, within EX31_RS21930 at 131.321 kb on - strand, within EX31_RS21930 at 131.376 kb on - strand, within EX31_RS21930 at 131.376 kb on - strand, within EX31_RS21930 at 131.399 kb on + strand, within EX31_RS21930 at 131.400 kb on - strand, within EX31_RS21930 at 131.400 kb on - strand, within EX31_RS21930 at 131.404 kb on - strand, within EX31_RS21930 at 131.409 kb on + strand, within EX31_RS21930 at 131.410 kb on - strand, within EX31_RS21930 at 131.410 kb on - strand, within EX31_RS21930 at 131.519 kb on + strand at 131.519 kb on + strand at 131.519 kb on + strand at 131.520 kb on - strand at 131.520 kb on - strand at 131.569 kb on + strand at 131.569 kb on + strand at 131.569 kb on + strand at 131.570 kb on - strand at 131.573 kb on + strand at 131.601 kb on - strand at 131.614 kb on - strand at 131.614 kb on - strand at 131.614 kb on - strand at 131.705 kb on + strand at 131.705 kb on + strand at 131.705 kb on + strand at 131.705 kb on + strand at 131.705 kb on + strand at 131.705 kb on + strand at 131.705 kb on + strand at 131.706 kb on - strand at 131.706 kb on - strand at 131.706 kb on - strand at 131.706 kb on - strand at 131.706 kb on - strand at 131.706 kb on - strand at 131.717 kb on - strand at 131.862 kb on + strand, within EX31_RS21935 at 131.893 kb on + strand, within EX31_RS21935 at 131.894 kb on - strand, within EX31_RS21935 at 131.894 kb on - strand, within EX31_RS21935
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; vial remove 129,219 - +0.6 129,225 - +0.9 129,281 + +1.3 129,282 - +0.1 129,373 + EX31_RS21920 0.14 -0.1 129,373 + EX31_RS21920 0.14 -0.8 129,374 - EX31_RS21920 0.14 +1.5 129,390 + EX31_RS21920 0.16 -0.5 129,418 - EX31_RS21920 0.20 -0.1 129,491 + EX31_RS21920 0.29 -1.4 129,491 + EX31_RS21920 0.29 -1.3 129,599 - EX31_RS21920 0.42 +0.3 129,623 + EX31_RS21920 0.45 +0.5 129,623 + EX31_RS21920 0.45 +0.3 129,624 - EX31_RS21920 0.45 +0.6 129,625 + EX31_RS21920 0.46 +0.6 129,625 + EX31_RS21920 0.46 -0.4 129,625 + EX31_RS21920 0.46 -0.1 129,626 - EX31_RS21920 0.46 -1.1 129,626 - EX31_RS21920 0.46 -0.8 129,720 - EX31_RS21920 0.57 -0.2 129,856 + EX31_RS21920 0.74 -0.2 129,856 + EX31_RS21920 0.74 -1.5 129,856 + EX31_RS21920 0.74 +0.1 129,856 + EX31_RS21920 0.74 -1.4 129,856 + EX31_RS21920 0.74 -0.0 129,856 + EX31_RS21920 0.74 -1.2 129,856 + EX31_RS21920 0.74 -1.1 129,856 + EX31_RS21920 0.74 -0.8 129,856 + EX31_RS21920 0.74 -1.2 129,857 - EX31_RS21920 0.74 +1.2 129,857 - EX31_RS21920 0.74 -0.1 129,857 - EX31_RS21920 0.74 -0.5 129,857 - EX31_RS21920 0.74 +1.1 129,857 - EX31_RS21920 0.74 -0.2 129,857 - EX31_RS21920 0.74 +0.1 129,906 + EX31_RS21920 0.80 +0.5 129,907 - EX31_RS21920 0.81 -0.8 129,907 - EX31_RS21920 0.81 -0.0 129,942 + EX31_RS21920 0.85 +0.0 129,942 + EX31_RS21920 0.85 -0.7 129,942 + EX31_RS21920 0.85 -0.6 129,942 + EX31_RS21920 0.85 -0.7 129,943 - EX31_RS21920 0.85 -0.1 129,946 + EX31_RS21920 0.85 -0.9 129,946 + EX31_RS21920 0.85 -0.1 129,946 + EX31_RS21920 0.85 -0.0 129,946 + EX31_RS21920 0.85 +1.3 129,947 - EX31_RS21920 0.86 -1.6 129,947 - EX31_RS21920 0.86 +0.0 129,947 - EX31_RS21920 0.86 +0.5 129,958 + EX31_RS21920 0.87 +0.6 129,959 - EX31_RS21920 0.87 +1.1 129,981 - EX31_RS21920 0.90 +0.6 130,008 + +0.7 130,008 + -0.6 130,008 + +0.0 130,009 - -0.7 130,142 + +0.1 130,171 + -0.8 130,188 - +0.1 130,560 - EX31_RS21925 0.53 -0.6 130,560 - EX31_RS21925 0.53 -1.4 130,560 - EX31_RS21925 0.53 -0.5 130,602 + EX31_RS21925 0.58 -1.0 130,602 + EX31_RS21925 0.58 -2.2 130,603 - EX31_RS21925 0.58 -0.4 130,657 - EX31_RS21925 0.65 -0.5 130,672 + EX31_RS21925 0.66 -0.4 130,673 - EX31_RS21925 0.67 -0.5 130,673 - EX31_RS21925 0.67 -0.3 130,795 + EX31_RS21925 0.81 -3.0 130,796 - EX31_RS21925 0.81 +0.3 130,796 - EX31_RS21925 0.81 +0.7 130,796 - EX31_RS21925 0.81 -0.3 130,796 - EX31_RS21925 0.81 -0.3 130,826 - EX31_RS21925 0.85 -3.1 130,853 + EX31_RS21925 0.88 +0.1 130,871 + -0.5 130,928 + -0.0 130,928 + +1.0 130,928 + -0.4 130,928 + -0.7 130,929 - -0.3 130,932 + -0.3 130,932 + -1.8 130,932 + -0.7 130,932 + +1.5 130,933 - +0.0 130,933 - -1.0 130,933 - -1.1 131,015 + +0.1 131,015 + -0.9 131,016 - +0.3 131,016 - -0.8 131,016 - +0.3 131,016 - -1.0 131,016 - +0.3 131,046 - EX31_RS21930 0.10 +0.1 131,048 + EX31_RS21930 0.11 -1.9 131,049 - EX31_RS21930 0.11 +0.4 131,049 - EX31_RS21930 0.11 -0.8 131,218 - EX31_RS21930 0.42 +0.1 131,220 - EX31_RS21930 0.42 -0.0 131,257 - EX31_RS21930 0.49 -0.2 131,257 - EX31_RS21930 0.49 +1.3 131,258 + EX31_RS21930 0.49 +1.5 131,320 + EX31_RS21930 0.60 +0.5 131,321 - EX31_RS21930 0.60 -0.2 131,321 - EX31_RS21930 0.60 -1.1 131,321 - EX31_RS21930 0.60 +0.1 131,321 - EX31_RS21930 0.60 +0.5 131,376 - EX31_RS21930 0.70 -0.0 131,376 - EX31_RS21930 0.70 -1.0 131,399 + EX31_RS21930 0.74 +0.1 131,400 - EX31_RS21930 0.75 +0.8 131,400 - EX31_RS21930 0.75 -0.6 131,404 - EX31_RS21930 0.75 +0.9 131,409 + EX31_RS21930 0.76 +0.4 131,410 - EX31_RS21930 0.77 -0.1 131,410 - EX31_RS21930 0.77 +0.3 131,519 + +0.6 131,519 + +0.4 131,519 + +0.2 131,520 - -0.3 131,520 - +0.1 131,569 + +1.2 131,569 + -0.9 131,569 + -0.8 131,570 - +0.5 131,573 + -0.8 131,601 - -0.0 131,614 - +1.3 131,614 - -1.6 131,614 - -0.7 131,705 + -0.3 131,705 + -1.4 131,705 + +0.9 131,705 + +0.7 131,705 + +0.0 131,705 + -0.2 131,705 + -0.8 131,706 - +0.2 131,706 - +0.0 131,706 - +0.3 131,706 - -1.4 131,706 - -0.3 131,706 - -0.7 131,717 - +0.3 131,862 + EX31_RS21935 0.23 -1.0 131,893 + EX31_RS21935 0.27 +2.3 131,894 - EX31_RS21935 0.28 -0.1 131,894 - EX31_RS21935 0.28 -0.2
Or see this region's nucleotide sequence