Strain Fitness in Rahnella sp. WP5 around EX31_RS15525

Experiment: NL-CCM; vial

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS15520 and EX31_RS15525 are separated by 369 nucleotidesEX31_RS15525 and EX31_RS15530 are separated by 140 nucleotidesEX31_RS15530 and EX31_RS15535 overlap by 4 nucleotides EX31_RS15520: EX31_RS15520 - pyridoxal phosphate-dependent aminotransferase, at 452 to 1,657 _RS15520 EX31_RS15525: EX31_RS15525 - nitrogen assimilation transcriptional regulator, at 2,027 to 2,950 _RS15525 EX31_RS15530: EX31_RS15530 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 3,091 to 3,531 _RS15530 EX31_RS15535: EX31_RS15535 - LamB/YcsF family protein, at 3,528 to 4,310 _RS15535 Position (kb) 2 3Strain fitness (log2 ratio) -2 -1 0 1 2at 1.039 kb on - strand, within EX31_RS15520at 1.039 kb on - strand, within EX31_RS15520at 1.082 kb on + strand, within EX31_RS15520at 1.082 kb on + strand, within EX31_RS15520at 1.275 kb on + strand, within EX31_RS15520at 1.276 kb on - strand, within EX31_RS15520at 1.276 kb on - strand, within EX31_RS15520at 1.392 kb on + strand, within EX31_RS15520at 1.393 kb on - strand, within EX31_RS15520at 1.546 kb on + strandat 1.690 kb on - strandat 1.729 kb on + strandat 1.729 kb on + strandat 1.730 kb on - strandat 1.778 kb on - strandat 1.888 kb on - strandat 1.896 kb on + strandat 1.952 kb on - strandat 1.987 kb on + strandat 1.987 kb on + strandat 1.988 kb on - strandat 1.988 kb on - strandat 1.991 kb on + strandat 2.220 kb on - strand, within EX31_RS15525at 2.501 kb on - strand, within EX31_RS15525at 2.501 kb on - strand, within EX31_RS15525at 2.591 kb on + strand, within EX31_RS15525at 2.591 kb on + strand, within EX31_RS15525at 2.591 kb on + strand, within EX31_RS15525at 2.592 kb on - strand, within EX31_RS15525at 2.592 kb on - strand, within EX31_RS15525at 2.839 kb on + strand, within EX31_RS15525at 2.948 kb on + strandat 3.011 kb on - strandat 3.011 kb on - strandat 3.011 kb on - strandat 3.011 kb on - strandat 3.022 kb on - strandat 3.205 kb on + strand, within EX31_RS15530at 3.320 kb on + strand, within EX31_RS15530at 3.534 kb on + strandat 3.534 kb on + strandat 3.576 kb on + strandat 3.700 kb on + strand, within EX31_RS15535at 3.700 kb on + strand, within EX31_RS15535at 3.700 kb on + strand, within EX31_RS15535at 3.700 kb on + strand, within EX31_RS15535at 3.700 kb on + strand, within EX31_RS15535at 3.700 kb on + strand, within EX31_RS15535at 3.786 kb on + strand, within EX31_RS15535at 3.786 kb on + strand, within EX31_RS15535at 3.787 kb on - strand, within EX31_RS15535at 3.787 kb on - strand, within EX31_RS15535at 3.787 kb on - strand, within EX31_RS15535at 3.796 kb on - strand, within EX31_RS15535at 3.879 kb on - strand, within EX31_RS15535

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Per-strain Table

Position Strand Gene LocusTag Fraction NL-CCM; vial
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1,039 - EX31_RS15520 0.49 -0.9
1,039 - EX31_RS15520 0.49 +1.8
1,082 + EX31_RS15520 0.52 -0.7
1,082 + EX31_RS15520 0.52 +0.0
1,275 + EX31_RS15520 0.68 -0.7
1,276 - EX31_RS15520 0.68 +2.0
1,276 - EX31_RS15520 0.68 -0.3
1,392 + EX31_RS15520 0.78 -1.0
1,393 - EX31_RS15520 0.78 -0.3
1,546 + -0.2
1,690 - +0.7
1,729 + -0.5
1,729 + -1.9
1,730 - +0.7
1,778 - -0.2
1,888 - -0.8
1,896 + -2.7
1,952 - -0.7
1,987 + -0.0
1,987 + +1.5
1,988 - +1.0
1,988 - +0.4
1,991 + +0.5
2,220 - EX31_RS15525 0.21 -0.5
2,501 - EX31_RS15525 0.51 +0.7
2,501 - EX31_RS15525 0.51 -0.3
2,591 + EX31_RS15525 0.61 -0.3
2,591 + EX31_RS15525 0.61 +0.4
2,591 + EX31_RS15525 0.61 -0.5
2,592 - EX31_RS15525 0.61 -2.5
2,592 - EX31_RS15525 0.61 -0.7
2,839 + EX31_RS15525 0.88 -1.2
2,948 + +0.7
3,011 - -0.4
3,011 - -0.8
3,011 - +0.2
3,011 - -1.2
3,022 - -0.1
3,205 + EX31_RS15530 0.26 -0.1
3,320 + EX31_RS15530 0.52 +0.2
3,534 + -0.2
3,534 + +0.7
3,576 + +0.3
3,700 + EX31_RS15535 0.22 -0.5
3,700 + EX31_RS15535 0.22 -0.6
3,700 + EX31_RS15535 0.22 +0.2
3,700 + EX31_RS15535 0.22 +0.3
3,700 + EX31_RS15535 0.22 +0.3
3,700 + EX31_RS15535 0.22 -0.3
3,786 + EX31_RS15535 0.33 -1.9
3,786 + EX31_RS15535 0.33 -0.8
3,787 - EX31_RS15535 0.33 -1.6
3,787 - EX31_RS15535 0.33 -2.3
3,787 - EX31_RS15535 0.33 -1.1
3,796 - EX31_RS15535 0.34 -0.4
3,879 - EX31_RS15535 0.45 +1.0

Or see this region's nucleotide sequence