Experiment: NL-CCM; vial
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS13165 and EX31_RS13170 are separated by 302 nucleotides EX31_RS13170 and EX31_RS13175 overlap by 8 nucleotides EX31_RS13175 and EX31_RS13180 are separated by 44 nucleotides
EX31_RS13165: EX31_RS13165 - pitrilysin, at 57,339 to 60,224
_RS13165
EX31_RS13170: EX31_RS13170 - precorrin-2 dehydrogenase, at 60,527 to 60,871
_RS13170
EX31_RS13175: EX31_RS13175 - YgdB family protein, at 60,864 to 61,301
_RS13175
EX31_RS13180: EX31_RS13180 - prepilin peptidase-dependent protein, at 61,346 to 61,933
_RS13180
Position (kb)
60
61 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 59.545 kb on + strand, within EX31_RS13165 at 59.545 kb on + strand, within EX31_RS13165 at 59.545 kb on + strand, within EX31_RS13165 at 59.545 kb on + strand, within EX31_RS13165 at 59.545 kb on + strand, within EX31_RS13165 at 59.546 kb on - strand, within EX31_RS13165 at 59.546 kb on - strand, within EX31_RS13165 at 59.546 kb on - strand, within EX31_RS13165 at 59.549 kb on - strand, within EX31_RS13165 at 59.558 kb on - strand, within EX31_RS13165 at 59.585 kb on + strand, within EX31_RS13165 at 59.585 kb on + strand, within EX31_RS13165 at 59.586 kb on - strand, within EX31_RS13165 at 59.586 kb on - strand, within EX31_RS13165 at 59.586 kb on - strand, within EX31_RS13165 at 59.597 kb on + strand, within EX31_RS13165 at 59.610 kb on - strand, within EX31_RS13165 at 59.655 kb on + strand, within EX31_RS13165 at 59.655 kb on + strand, within EX31_RS13165 at 59.656 kb on - strand, within EX31_RS13165 at 59.656 kb on - strand, within EX31_RS13165 at 59.656 kb on - strand, within EX31_RS13165 at 59.656 kb on - strand, within EX31_RS13165 at 59.732 kb on + strand, within EX31_RS13165 at 59.760 kb on + strand, within EX31_RS13165 at 59.760 kb on + strand, within EX31_RS13165 at 59.760 kb on + strand, within EX31_RS13165 at 59.761 kb on - strand, within EX31_RS13165 at 59.796 kb on + strand, within EX31_RS13165 at 59.796 kb on + strand, within EX31_RS13165 at 59.797 kb on - strand, within EX31_RS13165 at 59.797 kb on - strand, within EX31_RS13165 at 59.833 kb on + strand, within EX31_RS13165 at 59.849 kb on - strand, within EX31_RS13165 at 59.921 kb on - strand, within EX31_RS13165 at 59.921 kb on - strand, within EX31_RS13165 at 59.921 kb on - strand, within EX31_RS13165 at 59.940 kb on + strand at 59.954 kb on + strand at 59.954 kb on + strand at 59.954 kb on + strand at 59.955 kb on - strand at 59.956 kb on + strand at 59.957 kb on - strand at 59.957 kb on - strand at 59.957 kb on - strand at 59.957 kb on - strand at 59.957 kb on - strand at 60.065 kb on + strand at 60.065 kb on + strand at 60.066 kb on - strand at 60.066 kb on - strand at 60.072 kb on - strand at 60.080 kb on + strand at 60.080 kb on + strand at 60.081 kb on - strand at 60.116 kb on + strand at 60.117 kb on - strand at 60.122 kb on + strand at 60.122 kb on + strand at 60.122 kb on + strand at 60.122 kb on + strand at 60.123 kb on - strand at 60.123 kb on - strand at 60.219 kb on + strand at 60.266 kb on + strand at 60.267 kb on - strand at 60.267 kb on - strand at 60.267 kb on - strand at 60.270 kb on + strand at 60.271 kb on - strand at 60.272 kb on + strand at 60.272 kb on + strand at 60.272 kb on + strand at 60.273 kb on - strand at 60.273 kb on - strand at 60.273 kb on - strand at 60.285 kb on + strand at 60.285 kb on + strand at 60.285 kb on + strand at 60.286 kb on - strand at 60.286 kb on - strand at 60.326 kb on + strand at 60.348 kb on - strand at 60.382 kb on + strand at 60.382 kb on + strand at 60.382 kb on + strand at 60.382 kb on + strand at 60.383 kb on - strand at 60.383 kb on - strand at 60.383 kb on - strand at 60.383 kb on - strand at 60.383 kb on - strand at 60.383 kb on - strand at 60.406 kb on - strand at 60.422 kb on + strand at 60.423 kb on - strand at 60.425 kb on - strand at 60.444 kb on - strand at 60.448 kb on + strand at 60.449 kb on - strand at 60.449 kb on - strand at 60.453 kb on + strand at 60.453 kb on + strand at 60.453 kb on + strand at 60.453 kb on + strand at 60.453 kb on + strand at 60.454 kb on - strand at 60.454 kb on - strand at 60.454 kb on - strand at 60.454 kb on - strand at 60.454 kb on - strand at 60.454 kb on - strand at 60.483 kb on + strand at 60.483 kb on + strand at 60.483 kb on + strand at 60.483 kb on + strand at 60.483 kb on + strand at 60.484 kb on - strand at 60.502 kb on + strand at 60.506 kb on - strand at 60.506 kb on - strand at 60.514 kb on + strand at 60.515 kb on - strand at 60.515 kb on - strand at 60.546 kb on - strand at 60.548 kb on + strand at 60.549 kb on - strand at 60.549 kb on - strand at 60.560 kb on + strand at 60.560 kb on + strand at 60.634 kb on + strand, within EX31_RS13170 at 60.690 kb on + strand, within EX31_RS13170 at 60.691 kb on - strand, within EX31_RS13170 at 60.691 kb on - strand, within EX31_RS13170 at 60.691 kb on - strand, within EX31_RS13170 at 60.691 kb on - strand, within EX31_RS13170 at 60.691 kb on - strand, within EX31_RS13170 at 60.771 kb on + strand, within EX31_RS13170 at 60.771 kb on + strand, within EX31_RS13170 at 60.774 kb on + strand, within EX31_RS13170 at 60.775 kb on - strand, within EX31_RS13170 at 60.775 kb on - strand, within EX31_RS13170 at 60.775 kb on - strand, within EX31_RS13170 at 60.775 kb on - strand, within EX31_RS13170 at 60.775 kb on - strand, within EX31_RS13170 at 60.775 kb on - strand, within EX31_RS13170 at 60.976 kb on + strand, within EX31_RS13175 at 60.977 kb on - strand, within EX31_RS13175 at 60.983 kb on + strand, within EX31_RS13175 at 60.984 kb on - strand, within EX31_RS13175 at 61.034 kb on + strand, within EX31_RS13175 at 61.034 kb on + strand, within EX31_RS13175 at 61.035 kb on - strand, within EX31_RS13175 at 61.059 kb on - strand, within EX31_RS13175 at 61.161 kb on + strand, within EX31_RS13175 at 61.161 kb on + strand, within EX31_RS13175 at 61.161 kb on + strand, within EX31_RS13175 at 61.162 kb on - strand, within EX31_RS13175 at 61.195 kb on + strand, within EX31_RS13175 at 61.195 kb on + strand, within EX31_RS13175 at 61.195 kb on + strand, within EX31_RS13175 at 61.195 kb on + strand, within EX31_RS13175 at 61.196 kb on - strand, within EX31_RS13175 at 61.253 kb on + strand, within EX31_RS13175 at 61.254 kb on - strand, within EX31_RS13175 at 61.254 kb on - strand, within EX31_RS13175 at 61.289 kb on + strand at 61.349 kb on + strand at 61.350 kb on - strand at 61.350 kb on - strand at 61.350 kb on - strand at 61.350 kb on - strand at 61.431 kb on + strand, within EX31_RS13180 at 61.431 kb on + strand, within EX31_RS13180 at 61.431 kb on + strand, within EX31_RS13180 at 61.431 kb on + strand, within EX31_RS13180 at 61.431 kb on + strand, within EX31_RS13180 at 61.432 kb on - strand, within EX31_RS13180 at 61.432 kb on - strand, within EX31_RS13180 at 61.432 kb on - strand, within EX31_RS13180 at 61.458 kb on - strand, within EX31_RS13180 at 61.587 kb on + strand, within EX31_RS13180 at 61.588 kb on - strand, within EX31_RS13180 at 61.641 kb on + strand, within EX31_RS13180 at 61.641 kb on + strand, within EX31_RS13180 at 61.642 kb on - strand, within EX31_RS13180 at 61.642 kb on - strand, within EX31_RS13180 at 61.642 kb on - strand, within EX31_RS13180 at 61.642 kb on - strand, within EX31_RS13180 at 61.645 kb on + strand, within EX31_RS13180 at 61.645 kb on + strand, within EX31_RS13180 at 61.645 kb on + strand, within EX31_RS13180 at 61.645 kb on + strand, within EX31_RS13180 at 61.646 kb on - strand, within EX31_RS13180 at 61.646 kb on - strand, within EX31_RS13180 at 61.646 kb on - strand, within EX31_RS13180 at 61.741 kb on + strand, within EX31_RS13180 at 61.741 kb on + strand, within EX31_RS13180 at 61.762 kb on + strand, within EX31_RS13180 at 61.762 kb on + strand, within EX31_RS13180
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; vial remove 59,545 + EX31_RS13165 0.76 +0.9 59,545 + EX31_RS13165 0.76 +1.0 59,545 + EX31_RS13165 0.76 +0.7 59,545 + EX31_RS13165 0.76 +0.1 59,545 + EX31_RS13165 0.76 +0.9 59,546 - EX31_RS13165 0.76 -1.0 59,546 - EX31_RS13165 0.76 -0.5 59,546 - EX31_RS13165 0.76 +0.5 59,549 - EX31_RS13165 0.77 -0.6 59,558 - EX31_RS13165 0.77 +0.3 59,585 + EX31_RS13165 0.78 -0.6 59,585 + EX31_RS13165 0.78 +0.3 59,586 - EX31_RS13165 0.78 -0.2 59,586 - EX31_RS13165 0.78 -0.1 59,586 - EX31_RS13165 0.78 +0.4 59,597 + EX31_RS13165 0.78 +0.5 59,610 - EX31_RS13165 0.79 -0.1 59,655 + EX31_RS13165 0.80 -0.6 59,655 + EX31_RS13165 0.80 -0.2 59,656 - EX31_RS13165 0.80 +0.5 59,656 - EX31_RS13165 0.80 -0.3 59,656 - EX31_RS13165 0.80 -0.3 59,656 - EX31_RS13165 0.80 +0.7 59,732 + EX31_RS13165 0.83 -0.3 59,760 + EX31_RS13165 0.84 +0.9 59,760 + EX31_RS13165 0.84 -0.2 59,760 + EX31_RS13165 0.84 +0.7 59,761 - EX31_RS13165 0.84 -1.0 59,796 + EX31_RS13165 0.85 +0.0 59,796 + EX31_RS13165 0.85 +0.4 59,797 - EX31_RS13165 0.85 -0.0 59,797 - EX31_RS13165 0.85 +0.9 59,833 + EX31_RS13165 0.86 -0.9 59,849 - EX31_RS13165 0.87 +0.2 59,921 - EX31_RS13165 0.89 -0.5 59,921 - EX31_RS13165 0.89 -0.3 59,921 - EX31_RS13165 0.89 -0.3 59,940 + +0.4 59,954 + -0.2 59,954 + +0.5 59,954 + -0.5 59,955 - -1.4 59,956 + +0.3 59,957 - +0.1 59,957 - +0.8 59,957 - -0.5 59,957 - +0.3 59,957 - +0.4 60,065 + -0.7 60,065 + +0.4 60,066 - +0.3 60,066 - -0.3 60,072 - +0.5 60,080 + -0.0 60,080 + -0.8 60,081 - -0.6 60,116 + +0.9 60,117 - -0.4 60,122 + +1.2 60,122 + -0.7 60,122 + +0.5 60,122 + -0.3 60,123 - -0.2 60,123 - -2.2 60,219 + -0.6 60,266 + +0.0 60,267 - +0.6 60,267 - +0.4 60,267 - +0.7 60,270 + +0.8 60,271 - +1.0 60,272 + -0.9 60,272 + +0.0 60,272 + +0.3 60,273 - -0.3 60,273 - +0.3 60,273 - +1.1 60,285 + +0.8 60,285 + -0.0 60,285 + +0.7 60,286 - -0.4 60,286 - +0.6 60,326 + -0.1 60,348 - +0.1 60,382 + -0.3 60,382 + -0.2 60,382 + +0.1 60,382 + +1.1 60,383 - +0.6 60,383 - -1.0 60,383 - +1.0 60,383 - -0.6 60,383 - +0.1 60,383 - -0.0 60,406 - -0.3 60,422 + -2.0 60,423 - +0.6 60,425 - -3.3 60,444 - -0.0 60,448 + +0.1 60,449 - +0.2 60,449 - +1.0 60,453 + +0.2 60,453 + +0.3 60,453 + -0.5 60,453 + -1.7 60,453 + -1.4 60,454 - -0.6 60,454 - +0.0 60,454 - -0.3 60,454 - -0.2 60,454 - +0.3 60,454 - -1.4 60,483 + -1.6 60,483 + +0.9 60,483 + -0.5 60,483 + -0.5 60,483 + -0.8 60,484 - -0.3 60,502 + -1.8 60,506 - -1.0 60,506 - +0.1 60,514 + +0.0 60,515 - -0.6 60,515 - +0.6 60,546 - +0.2 60,548 + +0.9 60,549 - +0.0 60,549 - -0.4 60,560 + +0.1 60,560 + +0.0 60,634 + EX31_RS13170 0.31 -1.6 60,690 + EX31_RS13170 0.47 +0.5 60,691 - EX31_RS13170 0.48 -0.7 60,691 - EX31_RS13170 0.48 -0.4 60,691 - EX31_RS13170 0.48 +1.0 60,691 - EX31_RS13170 0.48 -0.8 60,691 - EX31_RS13170 0.48 -0.7 60,771 + EX31_RS13170 0.71 +0.9 60,771 + EX31_RS13170 0.71 +0.4 60,774 + EX31_RS13170 0.72 -2.1 60,775 - EX31_RS13170 0.72 -1.3 60,775 - EX31_RS13170 0.72 +0.2 60,775 - EX31_RS13170 0.72 -1.7 60,775 - EX31_RS13170 0.72 -0.1 60,775 - EX31_RS13170 0.72 -0.9 60,775 - EX31_RS13170 0.72 -0.9 60,976 + EX31_RS13175 0.26 -0.3 60,977 - EX31_RS13175 0.26 -0.4 60,983 + EX31_RS13175 0.27 +0.3 60,984 - EX31_RS13175 0.27 +0.4 61,034 + EX31_RS13175 0.39 +1.7 61,034 + EX31_RS13175 0.39 +0.2 61,035 - EX31_RS13175 0.39 -0.7 61,059 - EX31_RS13175 0.45 +0.1 61,161 + EX31_RS13175 0.68 +0.2 61,161 + EX31_RS13175 0.68 -0.8 61,161 + EX31_RS13175 0.68 +0.0 61,162 - EX31_RS13175 0.68 +0.4 61,195 + EX31_RS13175 0.76 -0.0 61,195 + EX31_RS13175 0.76 -0.3 61,195 + EX31_RS13175 0.76 +0.2 61,195 + EX31_RS13175 0.76 -1.6 61,196 - EX31_RS13175 0.76 +0.1 61,253 + EX31_RS13175 0.89 -2.5 61,254 - EX31_RS13175 0.89 +0.2 61,254 - EX31_RS13175 0.89 -0.3 61,289 + -1.1 61,349 + -0.2 61,350 - +0.6 61,350 - -0.8 61,350 - +0.6 61,350 - -1.6 61,431 + EX31_RS13180 0.14 -0.3 61,431 + EX31_RS13180 0.14 -0.5 61,431 + EX31_RS13180 0.14 +0.9 61,431 + EX31_RS13180 0.14 +1.1 61,431 + EX31_RS13180 0.14 +0.0 61,432 - EX31_RS13180 0.15 +0.2 61,432 - EX31_RS13180 0.15 +0.0 61,432 - EX31_RS13180 0.15 -0.0 61,458 - EX31_RS13180 0.19 -0.2 61,587 + EX31_RS13180 0.41 +0.4 61,588 - EX31_RS13180 0.41 -1.4 61,641 + EX31_RS13180 0.50 -0.2 61,641 + EX31_RS13180 0.50 +0.5 61,642 - EX31_RS13180 0.50 -0.5 61,642 - EX31_RS13180 0.50 +0.3 61,642 - EX31_RS13180 0.50 -0.1 61,642 - EX31_RS13180 0.50 -1.3 61,645 + EX31_RS13180 0.51 -0.4 61,645 + EX31_RS13180 0.51 +0.6 61,645 + EX31_RS13180 0.51 +1.0 61,645 + EX31_RS13180 0.51 -0.8 61,646 - EX31_RS13180 0.51 +0.4 61,646 - EX31_RS13180 0.51 -0.3 61,646 - EX31_RS13180 0.51 -0.3 61,741 + EX31_RS13180 0.67 +0.6 61,741 + EX31_RS13180 0.67 -0.1 61,762 + EX31_RS13180 0.71 +0.4 61,762 + EX31_RS13180 0.71 -0.7
Or see this region's nucleotide sequence