Experiment: NL-CCM; vial
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00670 and EX31_RS25040 are separated by 58 nucleotides EX31_RS25040 and EX31_RS00675 are separated by 170 nucleotides EX31_RS00675 and EX31_RS00680 are separated by 746 nucleotides
EX31_RS00670: EX31_RS00670 - pyrroloquinoline quinone biosynthesis protein PqqB, at 138,889 to 139,800
_RS00670
EX31_RS25040: EX31_RS25040 - pyrroloquinoline quinone precursor peptide PqqA, at 139,859 to 139,930
_RS25040
EX31_RS00675: EX31_RS00675 - membrane dipeptidase, at 140,101 to 141,138
_RS00675
EX31_RS00680: EX31_RS00680 - ferric-rhodotorulic acid/ferric-coprogen receptor FhuE, at 141,885 to 144,110
_RS00680
Position (kb)
140
141
142 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 139.165 kb on - strand, within EX31_RS00670 at 139.165 kb on - strand, within EX31_RS00670 at 139.215 kb on - strand, within EX31_RS00670 at 139.215 kb on - strand, within EX31_RS00670 at 139.290 kb on - strand, within EX31_RS00670 at 139.787 kb on - strand at 139.787 kb on - strand at 139.787 kb on - strand at 139.787 kb on - strand at 139.791 kb on + strand at 139.841 kb on - strand at 139.860 kb on + strand at 139.861 kb on - strand at 139.875 kb on + strand, within EX31_RS25040 at 139.875 kb on + strand, within EX31_RS25040 at 139.876 kb on - strand, within EX31_RS25040 at 139.889 kb on + strand, within EX31_RS25040 at 139.890 kb on - strand, within EX31_RS25040 at 139.896 kb on - strand, within EX31_RS25040 at 139.922 kb on - strand, within EX31_RS25040 at 139.922 kb on - strand, within EX31_RS25040 at 139.930 kb on + strand at 139.930 kb on + strand at 139.930 kb on + strand at 139.930 kb on + strand at 139.930 kb on + strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.934 kb on + strand at 139.934 kb on + strand at 139.934 kb on + strand at 139.934 kb on + strand at 139.935 kb on - strand at 139.935 kb on - strand at 139.935 kb on - strand at 139.935 kb on - strand at 139.935 kb on - strand at 139.935 kb on - strand at 139.951 kb on + strand at 139.959 kb on + strand at 139.959 kb on + strand at 139.960 kb on - strand at 139.977 kb on - strand at 139.998 kb on + strand at 139.999 kb on - strand at 139.999 kb on - strand at 139.999 kb on - strand at 139.999 kb on - strand at 139.999 kb on - strand at 140.009 kb on - strand at 140.064 kb on - strand at 140.102 kb on + strand at 140.292 kb on + strand, within EX31_RS00675 at 140.347 kb on - strand, within EX31_RS00675 at 140.458 kb on + strand, within EX31_RS00675 at 140.672 kb on - strand, within EX31_RS00675 at 140.763 kb on + strand, within EX31_RS00675 at 140.945 kb on + strand, within EX31_RS00675 at 140.974 kb on + strand, within EX31_RS00675 at 140.975 kb on - strand, within EX31_RS00675 at 140.975 kb on - strand, within EX31_RS00675 at 141.088 kb on + strand at 141.099 kb on + strand at 141.100 kb on - strand at 141.150 kb on + strand at 141.155 kb on + strand at 141.155 kb on + strand at 141.156 kb on - strand at 141.156 kb on - strand at 141.183 kb on + strand at 141.183 kb on + strand at 141.183 kb on + strand at 141.333 kb on + strand at 141.375 kb on - strand at 141.380 kb on - strand at 141.380 kb on - strand at 141.380 kb on - strand at 141.380 kb on - strand at 141.434 kb on + strand at 141.434 kb on + strand at 141.435 kb on - strand at 141.435 kb on - strand at 141.489 kb on - strand at 141.633 kb on + strand at 141.644 kb on + strand at 141.644 kb on + strand at 141.665 kb on - strand at 141.764 kb on - strand at 141.764 kb on - strand at 141.803 kb on + strand at 141.804 kb on - strand at 141.804 kb on - strand at 141.893 kb on + strand at 141.893 kb on + strand at 141.894 kb on - strand at 141.894 kb on - strand at 141.894 kb on - strand at 141.895 kb on + strand at 141.896 kb on - strand at 141.896 kb on - strand at 141.896 kb on - strand at 141.896 kb on - strand at 141.940 kb on + strand at 141.940 kb on + strand at 141.940 kb on + strand at 141.941 kb on - strand at 141.962 kb on - strand at 141.980 kb on + strand at 141.981 kb on - strand at 142.049 kb on - strand at 142.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; vial remove 139,165 - EX31_RS00670 0.30 +0.7 139,165 - EX31_RS00670 0.30 -0.7 139,215 - EX31_RS00670 0.36 -0.1 139,215 - EX31_RS00670 0.36 +0.2 139,290 - EX31_RS00670 0.44 -0.7 139,787 - -1.4 139,787 - +1.3 139,787 - +0.1 139,787 - +0.5 139,791 + -0.1 139,841 - -0.8 139,860 + +0.2 139,861 - -1.0 139,875 + EX31_RS25040 0.22 +1.9 139,875 + EX31_RS25040 0.22 +0.6 139,876 - EX31_RS25040 0.24 +0.9 139,889 + EX31_RS25040 0.42 +1.1 139,890 - EX31_RS25040 0.43 -0.9 139,896 - EX31_RS25040 0.51 +0.0 139,922 - EX31_RS25040 0.88 -0.6 139,922 - EX31_RS25040 0.88 -0.6 139,930 + -1.3 139,930 + -0.9 139,930 + +1.2 139,930 + -0.1 139,930 + +1.5 139,931 - +0.1 139,931 - -0.3 139,931 - +0.0 139,931 - -0.8 139,931 - +0.4 139,931 - +0.0 139,931 - -1.7 139,931 - -0.1 139,934 + -0.9 139,934 + -0.5 139,934 + +0.5 139,934 + +0.2 139,935 - -0.6 139,935 - -0.2 139,935 - +0.6 139,935 - +0.6 139,935 - +1.5 139,935 - +0.4 139,951 + +0.4 139,959 + -2.1 139,959 + +0.5 139,960 - +0.1 139,977 - +0.4 139,998 + -0.1 139,999 - -0.5 139,999 - -0.3 139,999 - -0.7 139,999 - +0.2 139,999 - +1.5 140,009 - +0.3 140,064 - +1.2 140,102 + +0.1 140,292 + EX31_RS00675 0.18 -1.0 140,347 - EX31_RS00675 0.24 -1.2 140,458 + EX31_RS00675 0.34 -2.6 140,672 - EX31_RS00675 0.55 -0.6 140,763 + EX31_RS00675 0.64 -0.3 140,945 + EX31_RS00675 0.81 -0.3 140,974 + EX31_RS00675 0.84 +0.2 140,975 - EX31_RS00675 0.84 -0.0 140,975 - EX31_RS00675 0.84 +0.6 141,088 + +0.2 141,099 + -2.1 141,100 - -0.7 141,150 + -1.5 141,155 + -1.2 141,155 + +0.7 141,156 - +1.3 141,156 - +1.1 141,183 + -1.2 141,183 + +0.1 141,183 + +0.3 141,333 + -0.9 141,375 - +0.5 141,380 - +1.3 141,380 - +0.7 141,380 - +0.3 141,380 - +2.0 141,434 + +0.1 141,434 + -0.1 141,435 - +0.2 141,435 - -0.2 141,489 - -1.1 141,633 + -0.4 141,644 + +1.6 141,644 + +0.5 141,665 - +0.4 141,764 - +0.6 141,764 - -0.0 141,803 + -1.2 141,804 - +0.6 141,804 - -0.1 141,893 + -1.7 141,893 + -1.2 141,894 - -0.2 141,894 - +0.1 141,894 - +0.4 141,895 + +0.6 141,896 - +0.3 141,896 - -0.5 141,896 - -0.7 141,896 - -0.4 141,940 + -0.6 141,940 + +0.9 141,940 + +0.2 141,941 - -0.4 141,962 - +1.4 141,980 + -0.1 141,981 - +1.3 142,049 - -0.6 142,049 - -1.3
Or see this region's nucleotide sequence