Experiment: NL-CCM_agar
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09720 and EX31_RS09725 are separated by 66 nucleotides EX31_RS09725 and EX31_RS09730 are separated by 318 nucleotides
EX31_RS09720: EX31_RS09720 - MFS transporter, at 144,739 to 146,022
_RS09720
EX31_RS09725: EX31_RS09725 - PQQ-dependent sugar dehydrogenase, at 146,089 to 147,240
_RS09725
EX31_RS09730: EX31_RS09730 - sugar ABC transporter substrate-binding protein, at 147,559 to 148,632
_RS09730
Position (kb) 146
147
148 Strain fitness (log2 ratio) -2
-1
0
1 at 145.115 kb on + strand, within EX31_RS09720 at 145.329 kb on + strand, within EX31_RS09720 at 145.370 kb on + strand, within EX31_RS09720 at 145.372 kb on + strand, within EX31_RS09720 at 145.372 kb on + strand, within EX31_RS09720 at 145.372 kb on + strand, within EX31_RS09720 at 145.372 kb on + strand, within EX31_RS09720 at 145.386 kb on + strand, within EX31_RS09720 at 145.500 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.514 kb on - strand, within EX31_RS09720 at 145.514 kb on - strand, within EX31_RS09720 at 145.514 kb on - strand, within EX31_RS09720 at 145.514 kb on - strand, within EX31_RS09720 at 145.514 kb on - strand, within EX31_RS09720 at 145.515 kb on + strand, within EX31_RS09720 at 145.515 kb on + strand, within EX31_RS09720 at 145.515 kb on + strand, within EX31_RS09720 at 145.516 kb on - strand, within EX31_RS09720 at 145.525 kb on + strand, within EX31_RS09720 at 145.525 kb on + strand, within EX31_RS09720 at 145.537 kb on + strand, within EX31_RS09720 at 145.680 kb on + strand, within EX31_RS09720 at 145.680 kb on + strand, within EX31_RS09720 at 145.681 kb on - strand, within EX31_RS09720 at 145.681 kb on - strand, within EX31_RS09720 at 145.865 kb on - strand, within EX31_RS09720 at 145.958 kb on - strand at 145.958 kb on - strand at 146.144 kb on + strand at 146.144 kb on + strand at 146.144 kb on + strand at 146.317 kb on - strand, within EX31_RS09725 at 146.319 kb on - strand, within EX31_RS09725 at 146.319 kb on - strand, within EX31_RS09725 at 146.361 kb on - strand, within EX31_RS09725 at 146.423 kb on + strand, within EX31_RS09725 at 146.424 kb on - strand, within EX31_RS09725 at 146.424 kb on - strand, within EX31_RS09725 at 146.457 kb on - strand, within EX31_RS09725 at 146.464 kb on - strand, within EX31_RS09725 at 146.541 kb on + strand, within EX31_RS09725 at 146.542 kb on - strand, within EX31_RS09725 at 146.542 kb on - strand, within EX31_RS09725 at 146.562 kb on + strand, within EX31_RS09725 at 146.795 kb on - strand, within EX31_RS09725 at 146.903 kb on + strand, within EX31_RS09725 at 146.903 kb on + strand, within EX31_RS09725 at 147.214 kb on + strand at 147.215 kb on - strand at 147.240 kb on + strand at 147.241 kb on - strand at 147.261 kb on + strand at 147.261 kb on + strand at 147.262 kb on - strand at 147.284 kb on - strand at 147.353 kb on - strand at 147.353 kb on - strand at 147.354 kb on + strand at 147.380 kb on - strand at 147.383 kb on + strand at 147.384 kb on - strand at 147.388 kb on + strand at 147.442 kb on + strand at 147.744 kb on + strand, within EX31_RS09730 at 147.778 kb on - strand, within EX31_RS09730 at 147.789 kb on + strand, within EX31_RS09730 at 147.789 kb on + strand, within EX31_RS09730 at 147.881 kb on - strand, within EX31_RS09730 at 147.918 kb on + strand, within EX31_RS09730 at 148.048 kb on - strand, within EX31_RS09730 at 148.106 kb on - strand, within EX31_RS09730 at 148.113 kb on + strand, within EX31_RS09730 at 148.113 kb on + strand, within EX31_RS09730 at 148.113 kb on + strand, within EX31_RS09730 at 148.191 kb on + strand, within EX31_RS09730
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM_agar remove 145,115 + EX31_RS09720 0.29 +0.9 145,329 + EX31_RS09720 0.46 -0.2 145,370 + EX31_RS09720 0.49 -0.1 145,372 + EX31_RS09720 0.49 -0.1 145,372 + EX31_RS09720 0.49 +0.4 145,372 + EX31_RS09720 0.49 -0.4 145,372 + EX31_RS09720 0.49 +0.6 145,386 + EX31_RS09720 0.50 +1.2 145,500 + EX31_RS09720 0.59 +0.2 145,513 + EX31_RS09720 0.60 +0.5 145,513 + EX31_RS09720 0.60 -1.8 145,513 + EX31_RS09720 0.60 -0.9 145,513 + EX31_RS09720 0.60 +0.2 145,513 + EX31_RS09720 0.60 +0.9 145,513 + EX31_RS09720 0.60 +0.8 145,513 + EX31_RS09720 0.60 -1.3 145,513 + EX31_RS09720 0.60 -1.4 145,513 + EX31_RS09720 0.60 +0.3 145,514 - EX31_RS09720 0.60 +0.3 145,514 - EX31_RS09720 0.60 -0.7 145,514 - EX31_RS09720 0.60 +0.9 145,514 - EX31_RS09720 0.60 +1.3 145,514 - EX31_RS09720 0.60 +0.7 145,515 + EX31_RS09720 0.60 +0.7 145,515 + EX31_RS09720 0.60 -0.4 145,515 + EX31_RS09720 0.60 -0.3 145,516 - EX31_RS09720 0.61 -0.0 145,525 + EX31_RS09720 0.61 +0.7 145,525 + EX31_RS09720 0.61 -0.7 145,537 + EX31_RS09720 0.62 -0.3 145,680 + EX31_RS09720 0.73 -1.3 145,680 + EX31_RS09720 0.73 -0.9 145,681 - EX31_RS09720 0.73 -0.2 145,681 - EX31_RS09720 0.73 +0.9 145,865 - EX31_RS09720 0.88 +1.3 145,958 - -0.6 145,958 - -1.3 146,144 + -0.8 146,144 + +1.3 146,144 + -0.6 146,317 - EX31_RS09725 0.20 -0.3 146,319 - EX31_RS09725 0.20 +0.4 146,319 - EX31_RS09725 0.20 +1.1 146,361 - EX31_RS09725 0.24 +0.3 146,423 + EX31_RS09725 0.29 -1.1 146,424 - EX31_RS09725 0.29 -0.1 146,424 - EX31_RS09725 0.29 +0.0 146,457 - EX31_RS09725 0.32 -0.3 146,464 - EX31_RS09725 0.33 -2.1 146,541 + EX31_RS09725 0.39 -0.0 146,542 - EX31_RS09725 0.39 -0.4 146,542 - EX31_RS09725 0.39 -0.2 146,562 + EX31_RS09725 0.41 -1.3 146,795 - EX31_RS09725 0.61 -0.1 146,903 + EX31_RS09725 0.71 -1.1 146,903 + EX31_RS09725 0.71 -1.0 147,214 + +0.0 147,215 - +0.7 147,240 + -1.1 147,241 - +1.0 147,261 + +0.9 147,261 + +0.3 147,262 - +0.7 147,284 - +0.3 147,353 - -0.5 147,353 - -0.1 147,354 + +0.2 147,380 - +0.1 147,383 + +0.4 147,384 - +0.5 147,388 + -0.0 147,442 + -0.2 147,744 + EX31_RS09730 0.17 -1.0 147,778 - EX31_RS09730 0.20 +0.2 147,789 + EX31_RS09730 0.21 -0.2 147,789 + EX31_RS09730 0.21 +1.6 147,881 - EX31_RS09730 0.30 -0.5 147,918 + EX31_RS09730 0.33 +0.3 148,048 - EX31_RS09730 0.46 -0.2 148,106 - EX31_RS09730 0.51 +0.4 148,113 + EX31_RS09730 0.52 -0.8 148,113 + EX31_RS09730 0.52 -0.1 148,113 + EX31_RS09730 0.52 -0.1 148,191 + EX31_RS09730 0.59 +1.6
Or see this region's nucleotide sequence