Experiment: NL-CCM_agar
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21980 and EX31_RS21985 are separated by 179 nucleotides EX31_RS21985 and EX31_RS21990 are separated by 58 nucleotides EX31_RS21990 and EX31_RS21995 are separated by 209 nucleotides
EX31_RS21980: EX31_RS21980 - AroM family protein, at 140,717 to 141,394
_RS21980
EX31_RS21985: EX31_RS21985 - pyrimidine/purine nucleoside phosphorylase, at 141,574 to 141,864
_RS21985
EX31_RS21990: EX31_RS21990 - recombination-associated protein RdgC, at 141,923 to 142,837
_RS21990
EX31_RS21995: EX31_RS21995 - fructokinase, at 143,047 to 143,967
_RS21995
Position (kb)
141
142
143 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 140.953 kb on - strand, within EX31_RS21980 at 141.037 kb on + strand, within EX31_RS21980 at 141.037 kb on + strand, within EX31_RS21980 at 141.038 kb on - strand, within EX31_RS21980 at 141.038 kb on - strand, within EX31_RS21980 at 141.070 kb on + strand, within EX31_RS21980 at 141.071 kb on - strand, within EX31_RS21980 at 141.093 kb on + strand, within EX31_RS21980 at 141.334 kb on + strand at 141.357 kb on + strand at 141.357 kb on + strand at 141.357 kb on + strand at 141.357 kb on + strand at 141.357 kb on + strand at 141.357 kb on + strand at 141.358 kb on - strand at 141.358 kb on - strand at 141.358 kb on - strand at 141.358 kb on - strand at 141.358 kb on - strand at 141.504 kb on + strand at 141.504 kb on + strand at 141.504 kb on + strand at 141.504 kb on + strand at 141.504 kb on + strand at 141.505 kb on - strand at 141.534 kb on + strand at 141.537 kb on + strand at 141.538 kb on - strand at 141.538 kb on - strand at 141.538 kb on - strand at 141.538 kb on - strand at 141.573 kb on + strand at 141.573 kb on + strand at 141.574 kb on - strand at 141.609 kb on - strand, within EX31_RS21985 at 141.676 kb on + strand, within EX31_RS21985 at 141.678 kb on + strand, within EX31_RS21985 at 141.679 kb on - strand, within EX31_RS21985 at 141.768 kb on + strand, within EX31_RS21985 at 141.769 kb on - strand, within EX31_RS21985 at 141.787 kb on + strand, within EX31_RS21985 at 141.787 kb on + strand, within EX31_RS21985 at 141.787 kb on + strand, within EX31_RS21985 at 141.787 kb on + strand, within EX31_RS21985 at 141.788 kb on - strand, within EX31_RS21985 at 141.788 kb on - strand, within EX31_RS21985 at 141.815 kb on + strand, within EX31_RS21985 at 141.815 kb on + strand, within EX31_RS21985 at 141.815 kb on + strand, within EX31_RS21985 at 141.815 kb on + strand, within EX31_RS21985 at 141.838 kb on + strand at 141.838 kb on + strand at 141.838 kb on + strand at 141.838 kb on + strand at 141.838 kb on + strand at 141.838 kb on + strand at 141.838 kb on + strand at 141.839 kb on - strand at 141.839 kb on - strand at 141.839 kb on - strand at 141.839 kb on - strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.846 kb on + strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.847 kb on - strand at 141.850 kb on + strand at 141.850 kb on + strand at 141.850 kb on + strand at 141.850 kb on + strand at 141.850 kb on + strand at 141.851 kb on - strand at 141.851 kb on - strand at 141.851 kb on - strand at 141.851 kb on - strand at 141.858 kb on + strand at 141.858 kb on + strand at 141.859 kb on - strand at 141.862 kb on + strand at 141.862 kb on + strand at 141.862 kb on + strand at 141.862 kb on + strand at 141.944 kb on + strand at 141.944 kb on + strand at 141.945 kb on - strand at 141.945 kb on - strand at 142.039 kb on + strand, within EX31_RS21990 at 142.085 kb on - strand, within EX31_RS21990 at 142.117 kb on + strand, within EX31_RS21990 at 142.118 kb on - strand, within EX31_RS21990 at 142.144 kb on + strand, within EX31_RS21990 at 142.144 kb on + strand, within EX31_RS21990 at 142.144 kb on + strand, within EX31_RS21990 at 142.145 kb on - strand, within EX31_RS21990 at 142.145 kb on - strand, within EX31_RS21990 at 142.145 kb on - strand, within EX31_RS21990 at 142.208 kb on - strand, within EX31_RS21990 at 142.268 kb on - strand, within EX31_RS21990 at 142.369 kb on + strand, within EX31_RS21990 at 142.583 kb on + strand, within EX31_RS21990 at 142.584 kb on - strand, within EX31_RS21990 at 142.594 kb on + strand, within EX31_RS21990 at 142.594 kb on + strand, within EX31_RS21990 at 142.595 kb on - strand, within EX31_RS21990 at 142.832 kb on + strand at 142.832 kb on + strand at 142.833 kb on - strand at 142.833 kb on - strand at 142.833 kb on - strand at 142.833 kb on - strand at 142.878 kb on - strand at 142.900 kb on + strand at 142.945 kb on + strand at 142.945 kb on + strand at 142.945 kb on + strand at 142.946 kb on - strand at 142.946 kb on - strand at 142.955 kb on + strand at 142.955 kb on + strand at 142.955 kb on + strand at 142.956 kb on - strand at 142.963 kb on + strand at 142.963 kb on + strand at 142.963 kb on + strand at 142.963 kb on + strand at 142.991 kb on + strand at 142.992 kb on - strand at 143.001 kb on - strand at 143.030 kb on + strand at 143.030 kb on + strand at 143.030 kb on + strand at 143.066 kb on + strand at 143.066 kb on + strand at 143.066 kb on + strand at 143.081 kb on + strand at 143.081 kb on + strand at 143.109 kb on + strand at 143.110 kb on - strand at 143.110 kb on - strand at 143.160 kb on + strand, within EX31_RS21995 at 143.160 kb on + strand, within EX31_RS21995 at 143.160 kb on + strand, within EX31_RS21995 at 143.161 kb on - strand, within EX31_RS21995 at 143.349 kb on + strand, within EX31_RS21995 at 143.349 kb on + strand, within EX31_RS21995 at 143.420 kb on + strand, within EX31_RS21995 at 143.421 kb on - strand, within EX31_RS21995 at 143.421 kb on - strand, within EX31_RS21995 at 143.421 kb on - strand, within EX31_RS21995 at 143.421 kb on - strand, within EX31_RS21995 at 143.518 kb on - strand, within EX31_RS21995 at 143.575 kb on + strand, within EX31_RS21995 at 143.635 kb on + strand, within EX31_RS21995 at 143.636 kb on - strand, within EX31_RS21995 at 143.636 kb on - strand, within EX31_RS21995 at 143.676 kb on + strand, within EX31_RS21995 at 143.676 kb on + strand, within EX31_RS21995 at 143.677 kb on - strand, within EX31_RS21995 at 143.677 kb on - strand, within EX31_RS21995 at 143.728 kb on + strand, within EX31_RS21995 at 143.729 kb on - strand, within EX31_RS21995 at 143.811 kb on + strand, within EX31_RS21995 at 143.811 kb on + strand, within EX31_RS21995 at 143.812 kb on - strand, within EX31_RS21995
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM_agar remove 140,953 - EX31_RS21980 0.35 -1.0 141,037 + EX31_RS21980 0.47 +0.2 141,037 + EX31_RS21980 0.47 +0.1 141,038 - EX31_RS21980 0.47 -0.2 141,038 - EX31_RS21980 0.47 +1.1 141,070 + EX31_RS21980 0.52 -0.8 141,071 - EX31_RS21980 0.52 +0.2 141,093 + EX31_RS21980 0.55 -0.8 141,334 + +0.4 141,357 + +0.1 141,357 + -0.5 141,357 + +0.3 141,357 + +0.1 141,357 + +0.1 141,357 + -0.5 141,358 - +0.3 141,358 - +0.1 141,358 - -0.3 141,358 - -0.2 141,358 - +0.2 141,504 + -1.7 141,504 + +1.0 141,504 + +0.1 141,504 + +0.6 141,504 + -0.2 141,505 - -0.0 141,534 + -0.2 141,537 + -0.7 141,538 - +0.3 141,538 - -0.0 141,538 - -0.1 141,538 - +0.2 141,573 + -0.9 141,573 + -0.8 141,574 - -0.2 141,609 - EX31_RS21985 0.12 +0.2 141,676 + EX31_RS21985 0.35 +1.0 141,678 + EX31_RS21985 0.36 -2.0 141,679 - EX31_RS21985 0.36 +1.5 141,768 + EX31_RS21985 0.67 +0.3 141,769 - EX31_RS21985 0.67 -0.5 141,787 + EX31_RS21985 0.73 -1.6 141,787 + EX31_RS21985 0.73 +0.2 141,787 + EX31_RS21985 0.73 -1.1 141,787 + EX31_RS21985 0.73 +0.1 141,788 - EX31_RS21985 0.74 +0.2 141,788 - EX31_RS21985 0.74 +0.4 141,815 + EX31_RS21985 0.83 +0.5 141,815 + EX31_RS21985 0.83 -0.1 141,815 + EX31_RS21985 0.83 -0.3 141,815 + EX31_RS21985 0.83 +0.7 141,838 + -0.7 141,838 + -0.6 141,838 + +0.6 141,838 + -0.2 141,838 + -1.0 141,838 + +0.4 141,838 + -0.3 141,839 - -0.2 141,839 - +0.4 141,839 - +1.1 141,839 - +0.8 141,846 + -0.0 141,846 + -0.1 141,846 + -0.7 141,847 - +0.3 141,847 - -1.1 141,847 - +1.9 141,847 - -0.9 141,847 - +0.5 141,850 + +0.9 141,850 + -0.7 141,850 + +0.4 141,850 + -0.9 141,850 + -0.3 141,851 - -0.6 141,851 - +0.5 141,851 - -2.1 141,851 - -1.3 141,858 + +1.3 141,858 + +0.2 141,859 - +0.7 141,862 + +0.0 141,862 + +0.9 141,862 + +0.5 141,862 + -0.8 141,944 + +0.2 141,944 + +0.7 141,945 - +0.3 141,945 - +1.1 142,039 + EX31_RS21990 0.13 -0.2 142,085 - EX31_RS21990 0.18 -0.5 142,117 + EX31_RS21990 0.21 -0.1 142,118 - EX31_RS21990 0.21 -0.6 142,144 + EX31_RS21990 0.24 +0.9 142,144 + EX31_RS21990 0.24 +0.1 142,144 + EX31_RS21990 0.24 -0.4 142,145 - EX31_RS21990 0.24 +1.0 142,145 - EX31_RS21990 0.24 -0.9 142,145 - EX31_RS21990 0.24 -0.7 142,208 - EX31_RS21990 0.31 -0.1 142,268 - EX31_RS21990 0.38 -0.1 142,369 + EX31_RS21990 0.49 -1.3 142,583 + EX31_RS21990 0.72 -0.9 142,584 - EX31_RS21990 0.72 +0.4 142,594 + EX31_RS21990 0.73 -1.1 142,594 + EX31_RS21990 0.73 -0.5 142,595 - EX31_RS21990 0.73 -1.5 142,832 + +0.1 142,832 + -1.0 142,833 - +0.3 142,833 - +0.6 142,833 - -1.1 142,833 - +0.1 142,878 - +0.4 142,900 + -0.8 142,945 + +0.3 142,945 + -0.6 142,945 + +1.5 142,946 - +0.3 142,946 - -1.0 142,955 + -0.6 142,955 + -0.4 142,955 + -0.4 142,956 - +0.5 142,963 + +0.1 142,963 + -0.2 142,963 + -0.4 142,963 + +0.6 142,991 + -0.2 142,992 - +0.8 143,001 - -0.4 143,030 + -0.1 143,030 + +0.2 143,030 + +0.4 143,066 + +0.1 143,066 + -0.6 143,066 + -0.4 143,081 + -0.2 143,081 + -0.1 143,109 + +0.4 143,110 - -0.6 143,110 - -0.4 143,160 + EX31_RS21995 0.12 +0.7 143,160 + EX31_RS21995 0.12 -0.7 143,160 + EX31_RS21995 0.12 +0.7 143,161 - EX31_RS21995 0.12 +0.9 143,349 + EX31_RS21995 0.33 -0.1 143,349 + EX31_RS21995 0.33 -0.1 143,420 + EX31_RS21995 0.40 +0.4 143,421 - EX31_RS21995 0.41 +0.6 143,421 - EX31_RS21995 0.41 -0.4 143,421 - EX31_RS21995 0.41 -0.2 143,421 - EX31_RS21995 0.41 -0.0 143,518 - EX31_RS21995 0.51 +1.2 143,575 + EX31_RS21995 0.57 -0.2 143,635 + EX31_RS21995 0.64 +0.3 143,636 - EX31_RS21995 0.64 +0.0 143,636 - EX31_RS21995 0.64 -1.0 143,676 + EX31_RS21995 0.68 -1.0 143,676 + EX31_RS21995 0.68 -0.4 143,677 - EX31_RS21995 0.68 -0.7 143,677 - EX31_RS21995 0.68 +0.9 143,728 + EX31_RS21995 0.74 -0.1 143,729 - EX31_RS21995 0.74 +1.0 143,811 + EX31_RS21995 0.83 -0.4 143,811 + EX31_RS21995 0.83 +0.9 143,812 - EX31_RS21995 0.83 +0.2
Or see this region's nucleotide sequence