Strain Fitness in Rahnella sp. WP5 around EX31_RS16680

Experiment: NL-CCM_agar

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS16670 and EX31_RS16675 are separated by 99 nucleotidesEX31_RS16675 and EX31_RS16680 are separated by 55 nucleotidesEX31_RS16680 and EX31_RS16685 overlap by 4 nucleotidesEX31_RS16685 and EX31_RS16690 are separated by 15 nucleotides EX31_RS16670: EX31_RS16670 - NAD(P)-dependent alcohol dehydrogenase, at 16,779 to 17,837 _RS16670 EX31_RS16675: EX31_RS16675 - histidine utilization repressor, at 17,937 to 18,686 _RS16675 EX31_RS16680: EX31_RS16680 - enoyl-CoA hydratase, at 18,742 to 19,512 _RS16680 EX31_RS16685: EX31_RS16685 - 3-hydroxyacyl-CoA dehydrogenase family protein, at 19,509 to 20,402 _RS16685 EX31_RS16690: EX31_RS16690 - CoA transferase, at 20,418 to 21,620 _RS16690 Position (kb) 18 19 20Strain fitness (log2 ratio) -2 -1 0 1at 17.756 kb on + strandat 17.756 kb on + strandat 17.813 kb on - strandat 17.869 kb on - strandat 17.873 kb on - strandat 17.874 kb on + strandat 17.875 kb on - strandat 17.945 kb on + strandat 17.980 kb on + strandat 18.110 kb on + strand, within EX31_RS16675at 18.110 kb on + strand, within EX31_RS16675at 18.111 kb on - strand, within EX31_RS16675at 18.111 kb on - strand, within EX31_RS16675at 18.184 kb on - strand, within EX31_RS16675at 18.470 kb on - strand, within EX31_RS16675at 18.504 kb on + strand, within EX31_RS16675at 18.621 kb on - strandat 18.622 kb on + strandat 18.622 kb on + strandat 18.623 kb on - strandat 18.623 kb on - strandat 18.623 kb on - strandat 18.700 kb on + strandat 18.701 kb on - strandat 18.701 kb on - strandat 18.701 kb on - strandat 18.701 kb on - strandat 18.743 kb on + strandat 18.843 kb on - strand, within EX31_RS16680at 19.280 kb on + strand, within EX31_RS16680at 19.281 kb on - strand, within EX31_RS16680at 19.281 kb on - strand, within EX31_RS16680at 19.378 kb on - strand, within EX31_RS16680at 19.778 kb on + strand, within EX31_RS16685at 19.785 kb on + strand, within EX31_RS16685at 19.858 kb on + strand, within EX31_RS16685at 19.859 kb on - strand, within EX31_RS16685at 19.859 kb on - strand, within EX31_RS16685at 19.896 kb on - strand, within EX31_RS16685at 19.956 kb on - strand, within EX31_RS16685at 20.340 kb on + strandat 20.405 kb on + strandat 20.406 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NL-CCM_agar
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17,756 + -1.3
17,756 + -1.7
17,813 - -1.2
17,869 - -0.3
17,873 - -0.3
17,874 + -0.1
17,875 - +0.4
17,945 + -0.7
17,980 + -1.1
18,110 + EX31_RS16675 0.23 -0.5
18,110 + EX31_RS16675 0.23 -1.2
18,111 - EX31_RS16675 0.23 -1.1
18,111 - EX31_RS16675 0.23 -0.7
18,184 - EX31_RS16675 0.33 +0.4
18,470 - EX31_RS16675 0.71 -0.0
18,504 + EX31_RS16675 0.76 -0.2
18,621 - -1.3
18,622 + -0.3
18,622 + -0.3
18,623 - -0.5
18,623 - +0.5
18,623 - +0.5
18,700 + -0.5
18,701 - +0.2
18,701 - -0.7
18,701 - -0.7
18,701 - -0.4
18,743 + -0.8
18,843 - EX31_RS16680 0.13 -2.3
19,280 + EX31_RS16680 0.70 -2.3
19,281 - EX31_RS16680 0.70 +0.5
19,281 - EX31_RS16680 0.70 -0.7
19,378 - EX31_RS16680 0.82 -0.2
19,778 + EX31_RS16685 0.30 -0.4
19,785 + EX31_RS16685 0.31 +0.5
19,858 + EX31_RS16685 0.39 -0.4
19,859 - EX31_RS16685 0.39 -0.4
19,859 - EX31_RS16685 0.39 +0.3
19,896 - EX31_RS16685 0.43 -1.3
19,956 - EX31_RS16685 0.50 +1.1
20,340 + -0.4
20,405 + -0.7
20,406 - -0.7

Or see this region's nucleotide sequence