Experiment: NL-CCM_agar
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09505 and EX31_RS09510 are separated by 159 nucleotides EX31_RS09510 and EX31_RS09515 are separated by 569 nucleotides EX31_RS09515 and EX31_RS09520 are separated by 80 nucleotides EX31_RS09520 and EX31_RS09525 are separated by 144 nucleotides
EX31_RS09505: EX31_RS09505 - hypothetical protein, at 107,346 to 107,615
_RS09505
EX31_RS09510: EX31_RS09510 - hypothetical protein, at 107,775 to 107,975
_RS09510
EX31_RS09515: EX31_RS09515 - GNAT family N-acetyltransferase, at 108,545 to 109,009
_RS09515
EX31_RS09520: EX31_RS09520 - hypothetical protein, at 109,090 to 109,329
_RS09520
EX31_RS09525: EX31_RS09525 - bestrophin, at 109,474 to 110,379
_RS09525
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 107.615 kb on + strand at 107.616 kb on - strand at 107.616 kb on - strand at 107.655 kb on - strand at 107.659 kb on + strand at 107.660 kb on - strand at 107.660 kb on - strand at 107.661 kb on + strand at 107.661 kb on + strand at 107.661 kb on + strand at 107.661 kb on + strand at 107.662 kb on - strand at 107.714 kb on + strand at 107.714 kb on + strand at 107.715 kb on - strand at 107.715 kb on - strand at 107.807 kb on - strand, within EX31_RS09510 at 107.954 kb on + strand, within EX31_RS09510 at 107.973 kb on + strand at 107.998 kb on + strand at 107.999 kb on - strand at 108.241 kb on - strand at 108.298 kb on + strand at 108.310 kb on + strand at 108.311 kb on - strand at 108.311 kb on - strand at 108.336 kb on - strand at 108.340 kb on - strand at 108.340 kb on - strand at 108.349 kb on - strand at 108.349 kb on - strand at 108.360 kb on - strand at 108.360 kb on - strand at 108.360 kb on - strand at 108.418 kb on - strand at 108.418 kb on - strand at 108.510 kb on + strand at 108.526 kb on + strand at 108.617 kb on + strand, within EX31_RS09515 at 108.617 kb on + strand, within EX31_RS09515 at 108.618 kb on - strand, within EX31_RS09515 at 108.658 kb on + strand, within EX31_RS09515 at 108.658 kb on + strand, within EX31_RS09515 at 108.658 kb on + strand, within EX31_RS09515 at 108.659 kb on - strand, within EX31_RS09515 at 108.794 kb on + strand, within EX31_RS09515 at 108.956 kb on + strand, within EX31_RS09515 at 108.986 kb on + strand at 108.986 kb on + strand at 108.986 kb on + strand at 108.986 kb on + strand at 108.986 kb on + strand at 108.986 kb on + strand at 108.987 kb on - strand at 108.987 kb on - strand at 108.988 kb on + strand at 108.988 kb on + strand at 109.007 kb on + strand at 109.007 kb on + strand at 109.007 kb on + strand at 109.008 kb on - strand at 109.036 kb on + strand at 109.036 kb on + strand at 109.040 kb on + strand at 109.041 kb on - strand at 109.041 kb on - strand at 109.091 kb on + strand at 109.091 kb on + strand at 109.092 kb on - strand at 109.240 kb on - strand, within EX31_RS09520 at 109.242 kb on + strand, within EX31_RS09520 at 109.243 kb on - strand, within EX31_RS09520 at 109.245 kb on + strand, within EX31_RS09520 at 109.246 kb on - strand, within EX31_RS09520 at 109.329 kb on + strand at 109.330 kb on - strand at 109.368 kb on + strand at 109.368 kb on + strand at 109.368 kb on + strand at 109.369 kb on - strand at 109.376 kb on - strand at 109.397 kb on + strand at 109.475 kb on + strand at 109.475 kb on + strand at 109.476 kb on - strand at 109.476 kb on - strand at 109.476 kb on - strand at 109.476 kb on - strand at 109.555 kb on + strand at 109.569 kb on + strand, within EX31_RS09525 at 109.569 kb on + strand, within EX31_RS09525 at 109.569 kb on + strand, within EX31_RS09525 at 109.570 kb on - strand, within EX31_RS09525 at 109.570 kb on - strand, within EX31_RS09525 at 109.570 kb on - strand, within EX31_RS09525 at 109.570 kb on - strand, within EX31_RS09525 at 109.570 kb on - strand, within EX31_RS09525 at 109.591 kb on - strand, within EX31_RS09525 at 109.633 kb on - strand, within EX31_RS09525 at 109.652 kb on + strand, within EX31_RS09525 at 109.653 kb on - strand, within EX31_RS09525 at 109.723 kb on - strand, within EX31_RS09525 at 109.723 kb on - strand, within EX31_RS09525 at 109.733 kb on + strand, within EX31_RS09525 at 109.733 kb on + strand, within EX31_RS09525 at 109.733 kb on + strand, within EX31_RS09525 at 109.734 kb on - strand, within EX31_RS09525 at 109.734 kb on - strand, within EX31_RS09525 at 109.735 kb on + strand, within EX31_RS09525 at 109.736 kb on - strand, within EX31_RS09525 at 109.761 kb on - strand, within EX31_RS09525 at 109.782 kb on + strand, within EX31_RS09525 at 109.823 kb on - strand, within EX31_RS09525 at 109.823 kb on - strand, within EX31_RS09525 at 109.845 kb on + strand, within EX31_RS09525 at 109.845 kb on + strand, within EX31_RS09525 at 109.847 kb on + strand, within EX31_RS09525 at 109.918 kb on - strand, within EX31_RS09525 at 109.918 kb on - strand, within EX31_RS09525 at 109.918 kb on - strand, within EX31_RS09525 at 109.918 kb on - strand, within EX31_RS09525
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM_agar remove 107,615 + +0.9 107,616 - +0.4 107,616 - -0.6 107,655 - -0.4 107,659 + -0.0 107,660 - -0.3 107,660 - +0.1 107,661 + -0.1 107,661 + +0.3 107,661 + -2.2 107,661 + +0.2 107,662 - -0.5 107,714 + -0.6 107,714 + -0.3 107,715 - -0.3 107,715 - +0.7 107,807 - EX31_RS09510 0.16 +0.6 107,954 + EX31_RS09510 0.89 +1.2 107,973 + +0.8 107,998 + +1.2 107,999 - -0.1 108,241 - -0.1 108,298 + +1.1 108,310 + +0.7 108,311 - -0.2 108,311 - +0.6 108,336 - +0.4 108,340 - +0.5 108,340 - +0.8 108,349 - -0.4 108,349 - -0.9 108,360 - +0.3 108,360 - -0.2 108,360 - +0.9 108,418 - +0.2 108,418 - -0.6 108,510 + +0.7 108,526 + -0.1 108,617 + EX31_RS09515 0.15 -0.6 108,617 + EX31_RS09515 0.15 +0.1 108,618 - EX31_RS09515 0.16 -0.1 108,658 + EX31_RS09515 0.24 -0.3 108,658 + EX31_RS09515 0.24 -0.9 108,658 + EX31_RS09515 0.24 -1.4 108,659 - EX31_RS09515 0.25 +0.6 108,794 + EX31_RS09515 0.54 -1.0 108,956 + EX31_RS09515 0.88 -0.1 108,986 + +0.3 108,986 + +0.0 108,986 + +1.0 108,986 + +0.5 108,986 + -0.4 108,986 + -0.4 108,987 - +1.5 108,987 - +0.8 108,988 + +0.1 108,988 + +1.4 109,007 + -0.3 109,007 + -0.3 109,007 + +0.3 109,008 - +0.6 109,036 + -0.0 109,036 + -0.1 109,040 + -0.3 109,041 - +0.2 109,041 - -1.8 109,091 + -2.2 109,091 + +0.3 109,092 - -0.0 109,240 - EX31_RS09520 0.62 -0.2 109,242 + EX31_RS09520 0.63 +0.9 109,243 - EX31_RS09520 0.64 +0.9 109,245 + EX31_RS09520 0.65 +0.7 109,246 - EX31_RS09520 0.65 +0.4 109,329 + +0.1 109,330 - -0.7 109,368 + -0.2 109,368 + -1.4 109,368 + -0.9 109,369 - -1.0 109,376 - -0.7 109,397 + +1.7 109,475 + -0.9 109,475 + +0.7 109,476 - +0.6 109,476 - -0.2 109,476 - +0.9 109,476 - -3.2 109,555 + -0.1 109,569 + EX31_RS09525 0.10 -0.2 109,569 + EX31_RS09525 0.10 +1.6 109,569 + EX31_RS09525 0.10 -1.2 109,570 - EX31_RS09525 0.11 -0.7 109,570 - EX31_RS09525 0.11 +1.6 109,570 - EX31_RS09525 0.11 +0.7 109,570 - EX31_RS09525 0.11 -0.8 109,570 - EX31_RS09525 0.11 -0.3 109,591 - EX31_RS09525 0.13 +0.3 109,633 - EX31_RS09525 0.18 -1.4 109,652 + EX31_RS09525 0.20 -2.0 109,653 - EX31_RS09525 0.20 +0.9 109,723 - EX31_RS09525 0.27 +0.3 109,723 - EX31_RS09525 0.27 -0.2 109,733 + EX31_RS09525 0.29 +0.1 109,733 + EX31_RS09525 0.29 -0.2 109,733 + EX31_RS09525 0.29 +1.6 109,734 - EX31_RS09525 0.29 +0.6 109,734 - EX31_RS09525 0.29 +0.0 109,735 + EX31_RS09525 0.29 -1.3 109,736 - EX31_RS09525 0.29 -0.5 109,761 - EX31_RS09525 0.32 -0.7 109,782 + EX31_RS09525 0.34 +0.2 109,823 - EX31_RS09525 0.39 -1.5 109,823 - EX31_RS09525 0.39 -0.8 109,845 + EX31_RS09525 0.41 -0.7 109,845 + EX31_RS09525 0.41 +0.4 109,847 + EX31_RS09525 0.41 -0.2 109,918 - EX31_RS09525 0.49 -1.2 109,918 - EX31_RS09525 0.49 +1.2 109,918 - EX31_RS09525 0.49 -0.1 109,918 - EX31_RS09525 0.49 -0.9
Or see this region's nucleotide sequence