Experiment: NL-CCM_agar
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02120 and EX31_RS02125 are separated by 47 nucleotides EX31_RS02125 and EX31_RS02130 overlap by 4 nucleotides EX31_RS02130 and EX31_RS02135 overlap by 8 nucleotides EX31_RS02135 and EX31_RS02140 are separated by 264 nucleotides
EX31_RS02120: EX31_RS02120 - MFS transporter, at 464,091 to 465,296
_RS02120
EX31_RS02125: EX31_RS02125 - helix-turn-helix transcriptional regulator, at 465,344 to 466,054
_RS02125
EX31_RS02130: EX31_RS02130 - amino acid-binding protein, at 466,051 to 466,443
_RS02130
EX31_RS02135: EX31_RS02135 - antibiotic biosynthesis monooxygenase, at 466,436 to 466,771
_RS02135
EX31_RS02140: EX31_RS02140 - DUF1852 domain-containing protein, at 467,036 to 468,019
_RS02140
Position (kb) 466
467 Strain fitness (log2 ratio) -2
-1
0
1 at 465.052 kb on + strand, within EX31_RS02120 at 465.053 kb on - strand, within EX31_RS02120 at 465.281 kb on + strand at 465.282 kb on - strand at 465.294 kb on + strand at 465.295 kb on - strand at 465.295 kb on - strand at 465.295 kb on - strand at 465.295 kb on - strand at 465.295 kb on - strand at 465.295 kb on - strand at 465.332 kb on + strand at 465.461 kb on + strand, within EX31_RS02125 at 465.646 kb on - strand, within EX31_RS02125 at 465.681 kb on + strand, within EX31_RS02125 at 465.682 kb on - strand, within EX31_RS02125 at 465.740 kb on - strand, within EX31_RS02125 at 465.740 kb on - strand, within EX31_RS02125 at 465.801 kb on + strand, within EX31_RS02125 at 465.802 kb on - strand, within EX31_RS02125 at 465.802 kb on - strand, within EX31_RS02125 at 465.802 kb on - strand, within EX31_RS02125 at 465.952 kb on + strand, within EX31_RS02125 at 465.975 kb on - strand, within EX31_RS02125 at 465.975 kb on - strand, within EX31_RS02125 at 466.119 kb on + strand, within EX31_RS02130 at 466.120 kb on - strand, within EX31_RS02130 at 466.142 kb on + strand, within EX31_RS02130 at 466.143 kb on - strand, within EX31_RS02130 at 466.143 kb on - strand, within EX31_RS02130 at 466.148 kb on - strand, within EX31_RS02130 at 466.303 kb on + strand, within EX31_RS02130 at 466.304 kb on - strand, within EX31_RS02130 at 466.384 kb on - strand, within EX31_RS02130 at 466.440 kb on - strand at 466.440 kb on - strand at 466.440 kb on - strand at 466.440 kb on - strand at 466.440 kb on - strand at 466.483 kb on - strand, within EX31_RS02135 at 466.483 kb on - strand, within EX31_RS02135 at 466.494 kb on - strand, within EX31_RS02135 at 466.508 kb on + strand, within EX31_RS02135 at 466.516 kb on - strand, within EX31_RS02135 at 466.666 kb on + strand, within EX31_RS02135 at 466.666 kb on + strand, within EX31_RS02135 at 466.804 kb on - strand at 466.853 kb on - strand at 467.032 kb on + strand at 467.087 kb on + strand at 467.088 kb on - strand at 467.090 kb on - strand at 467.104 kb on + strand at 467.333 kb on - strand, within EX31_RS02140 at 467.434 kb on + strand, within EX31_RS02140 at 467.434 kb on + strand, within EX31_RS02140
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM_agar remove 465,052 + EX31_RS02120 0.80 +0.1 465,053 - EX31_RS02120 0.80 +0.2 465,281 + -0.6 465,282 - +0.7 465,294 + -0.9 465,295 - +0.0 465,295 - -1.2 465,295 - -2.1 465,295 - -0.9 465,295 - -0.4 465,295 - +0.1 465,332 + +0.5 465,461 + EX31_RS02125 0.16 -0.5 465,646 - EX31_RS02125 0.42 +0.1 465,681 + EX31_RS02125 0.47 +0.5 465,682 - EX31_RS02125 0.48 -0.3 465,740 - EX31_RS02125 0.56 -0.5 465,740 - EX31_RS02125 0.56 +0.3 465,801 + EX31_RS02125 0.64 -0.1 465,802 - EX31_RS02125 0.64 +1.7 465,802 - EX31_RS02125 0.64 -0.1 465,802 - EX31_RS02125 0.64 -0.4 465,952 + EX31_RS02125 0.86 -0.7 465,975 - EX31_RS02125 0.89 -0.9 465,975 - EX31_RS02125 0.89 -0.7 466,119 + EX31_RS02130 0.17 -0.4 466,120 - EX31_RS02130 0.18 -0.9 466,142 + EX31_RS02130 0.23 -0.2 466,143 - EX31_RS02130 0.23 -0.0 466,143 - EX31_RS02130 0.23 -2.3 466,148 - EX31_RS02130 0.25 -1.1 466,303 + EX31_RS02130 0.64 +0.0 466,304 - EX31_RS02130 0.64 -0.7 466,384 - EX31_RS02130 0.85 -0.7 466,440 - +0.4 466,440 - +1.2 466,440 - +0.3 466,440 - +0.2 466,440 - -1.2 466,483 - EX31_RS02135 0.14 +0.3 466,483 - EX31_RS02135 0.14 -0.9 466,494 - EX31_RS02135 0.17 +0.2 466,508 + EX31_RS02135 0.21 +0.1 466,516 - EX31_RS02135 0.24 -0.2 466,666 + EX31_RS02135 0.68 +0.5 466,666 + EX31_RS02135 0.68 -2.5 466,804 - +0.1 466,853 - -0.4 467,032 + -1.2 467,087 + -0.8 467,088 - +0.8 467,090 - +0.9 467,104 + -0.5 467,333 - EX31_RS02140 0.30 -0.9 467,434 + EX31_RS02140 0.40 -1.2 467,434 + EX31_RS02140 0.40 -0.1
Or see this region's nucleotide sequence