Strain Fitness in Rahnella sp. WP5 around EX31_RS01035

Experiment: NL-CCM_agar

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS01030 and EX31_RS01035 are separated by 52 nucleotidesEX31_RS01035 and EX31_RS01040 are separated by 154 nucleotides EX31_RS01030: EX31_RS01030 - low affinity potassium transporter Kup, at 225,959 to 227,833 _RS01030 EX31_RS01035: EX31_RS01035 - hypothetical protein, at 227,886 to 228,695 _RS01035 EX31_RS01040: EX31_RS01040 - serine hydrolase, at 228,850 to 229,737 _RS01040 Position (kb) 227 228 229Strain fitness (log2 ratio) -2 -1 0 1at 226.919 kb on + strand, within EX31_RS01030at 226.919 kb on + strand, within EX31_RS01030at 226.920 kb on - strand, within EX31_RS01030at 226.943 kb on + strand, within EX31_RS01030at 226.943 kb on + strand, within EX31_RS01030at 226.973 kb on + strand, within EX31_RS01030at 227.065 kb on + strand, within EX31_RS01030at 227.187 kb on + strand, within EX31_RS01030at 227.188 kb on - strand, within EX31_RS01030at 227.188 kb on - strand, within EX31_RS01030at 227.414 kb on + strand, within EX31_RS01030at 227.461 kb on - strand, within EX31_RS01030at 227.510 kb on + strand, within EX31_RS01030at 227.519 kb on - strand, within EX31_RS01030at 227.523 kb on + strand, within EX31_RS01030at 227.524 kb on - strand, within EX31_RS01030at 227.524 kb on - strand, within EX31_RS01030at 227.524 kb on - strand, within EX31_RS01030at 227.689 kb on + strandat 227.689 kb on + strandat 227.737 kb on - strandat 227.780 kb on + strandat 227.785 kb on + strandat 227.888 kb on - strandat 227.911 kb on - strandat 227.921 kb on + strandat 227.922 kb on - strandat 228.120 kb on + strand, within EX31_RS01035at 228.362 kb on - strand, within EX31_RS01035at 228.362 kb on - strand, within EX31_RS01035at 228.366 kb on + strand, within EX31_RS01035at 228.366 kb on + strand, within EX31_RS01035at 228.366 kb on + strand, within EX31_RS01035at 228.366 kb on + strand, within EX31_RS01035at 228.366 kb on + strand, within EX31_RS01035at 228.367 kb on - strand, within EX31_RS01035at 228.367 kb on - strand, within EX31_RS01035at 228.367 kb on - strand, within EX31_RS01035at 228.443 kb on - strand, within EX31_RS01035at 228.443 kb on - strand, within EX31_RS01035at 228.455 kb on + strand, within EX31_RS01035at 228.455 kb on + strand, within EX31_RS01035at 228.456 kb on - strand, within EX31_RS01035at 228.456 kb on - strand, within EX31_RS01035at 228.456 kb on - strand, within EX31_RS01035at 228.456 kb on - strand, within EX31_RS01035at 228.478 kb on - strand, within EX31_RS01035at 228.478 kb on - strand, within EX31_RS01035at 228.518 kb on + strand, within EX31_RS01035at 228.519 kb on - strand, within EX31_RS01035at 228.535 kb on + strand, within EX31_RS01035at 228.535 kb on + strand, within EX31_RS01035at 228.535 kb on + strand, within EX31_RS01035at 228.535 kb on + strand, within EX31_RS01035at 228.535 kb on + strand, within EX31_RS01035at 228.535 kb on + strand, within EX31_RS01035at 228.535 kb on + strand, within EX31_RS01035at 228.535 kb on + strand, within EX31_RS01035at 228.535 kb on + strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.536 kb on - strand, within EX31_RS01035at 228.654 kb on + strandat 228.655 kb on - strandat 228.689 kb on - strandat 228.689 kb on - strandat 228.696 kb on - strandat 228.696 kb on - strandat 228.696 kb on - strandat 228.735 kb on + strandat 228.741 kb on + strandat 228.741 kb on + strandat 228.741 kb on + strandat 228.742 kb on - strandat 228.742 kb on - strandat 228.742 kb on - strandat 228.743 kb on + strandat 228.743 kb on + strandat 228.743 kb on + strandat 228.805 kb on + strandat 228.805 kb on + strandat 228.805 kb on + strandat 228.806 kb on - strandat 228.868 kb on + strandat 228.869 kb on - strandat 228.869 kb on - strandat 228.874 kb on - strandat 229.024 kb on + strand, within EX31_RS01040at 229.024 kb on + strand, within EX31_RS01040at 229.026 kb on + strand, within EX31_RS01040at 229.026 kb on + strand, within EX31_RS01040at 229.027 kb on - strand, within EX31_RS01040at 229.027 kb on - strand, within EX31_RS01040at 229.027 kb on - strand, within EX31_RS01040at 229.027 kb on - strand, within EX31_RS01040at 229.087 kb on + strand, within EX31_RS01040at 229.363 kb on + strand, within EX31_RS01040at 229.364 kb on - strand, within EX31_RS01040at 229.381 kb on + strand, within EX31_RS01040at 229.382 kb on - strand, within EX31_RS01040at 229.437 kb on - strand, within EX31_RS01040at 229.489 kb on + strand, within EX31_RS01040at 229.627 kb on + strand, within EX31_RS01040at 229.671 kb on + strandat 229.682 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NL-CCM_agar
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226,919 + EX31_RS01030 0.51 -0.3
226,919 + EX31_RS01030 0.51 -0.2
226,920 - EX31_RS01030 0.51 +0.2
226,943 + EX31_RS01030 0.52 -0.1
226,943 + EX31_RS01030 0.52 +0.3
226,973 + EX31_RS01030 0.54 -0.4
227,065 + EX31_RS01030 0.59 +0.3
227,187 + EX31_RS01030 0.65 -1.1
227,188 - EX31_RS01030 0.66 +0.4
227,188 - EX31_RS01030 0.66 -0.7
227,414 + EX31_RS01030 0.78 -0.6
227,461 - EX31_RS01030 0.80 +0.3
227,510 + EX31_RS01030 0.83 -1.8
227,519 - EX31_RS01030 0.83 +0.2
227,523 + EX31_RS01030 0.83 -0.3
227,524 - EX31_RS01030 0.83 -0.3
227,524 - EX31_RS01030 0.83 -1.2
227,524 - EX31_RS01030 0.83 +1.2
227,689 + +0.0
227,689 + -0.2
227,737 - -1.0
227,780 + -2.1
227,785 + +0.8
227,888 - +0.1
227,911 - +1.0
227,921 + -0.6
227,922 - +0.3
228,120 + EX31_RS01035 0.29 -0.1
228,362 - EX31_RS01035 0.59 +0.3
228,362 - EX31_RS01035 0.59 -1.0
228,366 + EX31_RS01035 0.59 -1.2
228,366 + EX31_RS01035 0.59 -1.0
228,366 + EX31_RS01035 0.59 -0.3
228,366 + EX31_RS01035 0.59 -1.4
228,366 + EX31_RS01035 0.59 -0.8
228,367 - EX31_RS01035 0.59 -0.4
228,367 - EX31_RS01035 0.59 -0.2
228,367 - EX31_RS01035 0.59 +0.3
228,443 - EX31_RS01035 0.69 -0.1
228,443 - EX31_RS01035 0.69 +0.1
228,455 + EX31_RS01035 0.70 -0.1
228,455 + EX31_RS01035 0.70 -0.7
228,456 - EX31_RS01035 0.70 +0.1
228,456 - EX31_RS01035 0.70 -0.2
228,456 - EX31_RS01035 0.70 -0.9
228,456 - EX31_RS01035 0.70 -0.5
228,478 - EX31_RS01035 0.73 -0.4
228,478 - EX31_RS01035 0.73 +0.0
228,518 + EX31_RS01035 0.78 -1.4
228,519 - EX31_RS01035 0.78 -1.1
228,535 + EX31_RS01035 0.80 +1.5
228,535 + EX31_RS01035 0.80 -1.1
228,535 + EX31_RS01035 0.80 +0.3
228,535 + EX31_RS01035 0.80 -0.2
228,535 + EX31_RS01035 0.80 -1.0
228,535 + EX31_RS01035 0.80 -0.9
228,535 + EX31_RS01035 0.80 -0.0
228,535 + EX31_RS01035 0.80 -0.2
228,535 + EX31_RS01035 0.80 +0.2
228,536 - EX31_RS01035 0.80 +0.1
228,536 - EX31_RS01035 0.80 -0.7
228,536 - EX31_RS01035 0.80 -0.1
228,536 - EX31_RS01035 0.80 -0.5
228,536 - EX31_RS01035 0.80 -0.9
228,536 - EX31_RS01035 0.80 -0.2
228,536 - EX31_RS01035 0.80 +0.3
228,536 - EX31_RS01035 0.80 -0.5
228,536 - EX31_RS01035 0.80 +0.1
228,536 - EX31_RS01035 0.80 -0.5
228,536 - EX31_RS01035 0.80 -0.7
228,536 - EX31_RS01035 0.80 -0.3
228,536 - EX31_RS01035 0.80 +0.3
228,654 + -0.7
228,655 - -0.8
228,689 - +0.7
228,689 - -0.2
228,696 - +0.0
228,696 - +1.1
228,696 - +1.1
228,735 + -0.8
228,741 + -0.8
228,741 + -0.3
228,741 + +0.9
228,742 - -0.2
228,742 - +0.5
228,742 - +0.3
228,743 + -1.4
228,743 + -0.0
228,743 + -1.7
228,805 + -1.0
228,805 + -0.6
228,805 + -0.1
228,806 - -1.0
228,868 + -0.1
228,869 - -0.9
228,869 - +0.2
228,874 - +0.5
229,024 + EX31_RS01040 0.20 -0.5
229,024 + EX31_RS01040 0.20 +0.4
229,026 + EX31_RS01040 0.20 -1.3
229,026 + EX31_RS01040 0.20 -0.4
229,027 - EX31_RS01040 0.20 +0.2
229,027 - EX31_RS01040 0.20 -0.3
229,027 - EX31_RS01040 0.20 +0.4
229,027 - EX31_RS01040 0.20 +1.6
229,087 + EX31_RS01040 0.27 +0.0
229,363 + EX31_RS01040 0.58 -0.4
229,364 - EX31_RS01040 0.58 -0.6
229,381 + EX31_RS01040 0.60 -1.5
229,382 - EX31_RS01040 0.60 +1.0
229,437 - EX31_RS01040 0.66 +0.2
229,489 + EX31_RS01040 0.72 -0.5
229,627 + EX31_RS01040 0.88 -0.1
229,671 + +0.3
229,682 - -0.0

Or see this region's nucleotide sequence