Experiment: NL-CCM; Mixed culture; Sphingobium sp. WW5 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10200 and EX31_RS10205 overlap by 4 nucleotides EX31_RS10205 and EX31_RS10210 are separated by 9 nucleotides EX31_RS10210 and EX31_RS10215 overlap by 8 nucleotides
EX31_RS10200: EX31_RS10200 - 4-oxalmesaconate hydratase, at 253,380 to 254,126
_RS10200
EX31_RS10205: EX31_RS10205 - 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase, at 254,123 to 254,848
_RS10205
EX31_RS10210: EX31_RS10210 - 4-oxalomesaconate tautomerase, at 254,858 to 255,982
_RS10210
EX31_RS10215: EX31_RS10215 - NAD(P)-dependent oxidoreductase, at 255,975 to 256,859
_RS10215
Position (kb) 254
255
256 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 253.995 kb on + strand, within EX31_RS10200 at 253.996 kb on - strand, within EX31_RS10200 at 253.998 kb on + strand, within EX31_RS10200 at 253.998 kb on + strand, within EX31_RS10200 at 253.999 kb on - strand, within EX31_RS10200 at 254.039 kb on + strand, within EX31_RS10200 at 254.039 kb on + strand, within EX31_RS10200 at 254.119 kb on + strand at 254.120 kb on - strand at 254.120 kb on - strand at 254.161 kb on + strand at 254.177 kb on - strand at 254.220 kb on + strand, within EX31_RS10205 at 254.221 kb on - strand, within EX31_RS10205 at 254.271 kb on + strand, within EX31_RS10205 at 254.551 kb on + strand, within EX31_RS10205 at 254.551 kb on + strand, within EX31_RS10205 at 254.552 kb on - strand, within EX31_RS10205 at 254.607 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.766 kb on - strand, within EX31_RS10205 at 254.766 kb on - strand, within EX31_RS10205 at 254.766 kb on - strand, within EX31_RS10205 at 254.770 kb on + strand, within EX31_RS10205 at 254.770 kb on + strand, within EX31_RS10205 at 254.770 kb on + strand, within EX31_RS10205 at 255.103 kb on + strand, within EX31_RS10210 at 255.104 kb on - strand, within EX31_RS10210 at 255.148 kb on + strand, within EX31_RS10210 at 255.211 kb on + strand, within EX31_RS10210 at 255.290 kb on + strand, within EX31_RS10210 at 255.290 kb on + strand, within EX31_RS10210 at 255.668 kb on - strand, within EX31_RS10210 at 255.668 kb on - strand, within EX31_RS10210 at 255.700 kb on - strand, within EX31_RS10210 at 255.767 kb on - strand, within EX31_RS10210 at 255.767 kb on - strand, within EX31_RS10210 at 255.831 kb on + strand, within EX31_RS10210 at 255.831 kb on + strand, within EX31_RS10210 at 255.886 kb on - strand at 255.886 kb on - strand at 255.890 kb on - strand at 255.981 kb on - strand at 256.082 kb on + strand, within EX31_RS10215 at 256.082 kb on + strand, within EX31_RS10215 at 256.083 kb on - strand, within EX31_RS10215 at 256.083 kb on - strand, within EX31_RS10215 at 256.332 kb on + strand, within EX31_RS10215 at 256.333 kb on - strand, within EX31_RS10215 at 256.410 kb on + strand, within EX31_RS10215 at 256.753 kb on + strand, within EX31_RS10215 at 256.753 kb on + strand, within EX31_RS10215 at 256.754 kb on - strand, within EX31_RS10215 at 256.754 kb on - strand, within EX31_RS10215 at 256.804 kb on - strand at 256.804 kb on - strand at 256.805 kb on + strand at 256.806 kb on - strand at 256.840 kb on + strand at 256.857 kb on + strand at 256.858 kb on - strand at 256.858 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. WW5 2:1 starting OD to Azospirillum remove 253,995 + EX31_RS10200 0.82 +0.3 253,996 - EX31_RS10200 0.82 +1.2 253,998 + EX31_RS10200 0.83 +0.2 253,998 + EX31_RS10200 0.83 -1.6 253,999 - EX31_RS10200 0.83 +1.8 254,039 + EX31_RS10200 0.88 -0.2 254,039 + EX31_RS10200 0.88 +0.0 254,119 + -0.0 254,120 - +0.5 254,120 - +2.0 254,161 + +1.9 254,177 - +0.4 254,220 + EX31_RS10205 0.13 -1.2 254,221 - EX31_RS10205 0.13 -0.3 254,271 + EX31_RS10205 0.20 +0.3 254,551 + EX31_RS10205 0.59 -2.1 254,551 + EX31_RS10205 0.59 -0.9 254,552 - EX31_RS10205 0.59 -0.2 254,607 + EX31_RS10205 0.67 +0.4 254,765 + EX31_RS10205 0.88 +0.4 254,765 + EX31_RS10205 0.88 +0.1 254,765 + EX31_RS10205 0.88 -0.6 254,765 + EX31_RS10205 0.88 +0.5 254,765 + EX31_RS10205 0.88 +0.3 254,765 + EX31_RS10205 0.88 -0.3 254,766 - EX31_RS10205 0.89 +0.2 254,766 - EX31_RS10205 0.89 +0.7 254,766 - EX31_RS10205 0.89 +0.9 254,770 + EX31_RS10205 0.89 +0.3 254,770 + EX31_RS10205 0.89 -1.4 254,770 + EX31_RS10205 0.89 +1.3 255,103 + EX31_RS10210 0.22 +0.6 255,104 - EX31_RS10210 0.22 -0.1 255,148 + EX31_RS10210 0.26 -1.0 255,211 + EX31_RS10210 0.31 -0.0 255,290 + EX31_RS10210 0.38 -0.1 255,290 + EX31_RS10210 0.38 -0.7 255,668 - EX31_RS10210 0.72 -1.1 255,668 - EX31_RS10210 0.72 +0.3 255,700 - EX31_RS10210 0.75 -2.2 255,767 - EX31_RS10210 0.81 -3.2 255,767 - EX31_RS10210 0.81 -2.4 255,831 + EX31_RS10210 0.86 -0.9 255,831 + EX31_RS10210 0.86 -1.0 255,886 - +0.9 255,886 - +0.1 255,890 - -0.3 255,981 - +1.4 256,082 + EX31_RS10215 0.12 -0.5 256,082 + EX31_RS10215 0.12 -0.6 256,083 - EX31_RS10215 0.12 -1.1 256,083 - EX31_RS10215 0.12 +0.5 256,332 + EX31_RS10215 0.40 +0.3 256,333 - EX31_RS10215 0.40 -0.5 256,410 + EX31_RS10215 0.49 -2.1 256,753 + EX31_RS10215 0.88 +1.9 256,753 + EX31_RS10215 0.88 -0.3 256,754 - EX31_RS10215 0.88 -0.1 256,754 - EX31_RS10215 0.88 -0.5 256,804 - +0.7 256,804 - +0.5 256,805 + -0.3 256,806 - +0.3 256,840 + +0.1 256,857 + +1.1 256,858 - -0.1 256,858 - -0.1
Or see this region's nucleotide sequence