Experiment: NL-CCM; Mixed culture; Sphingobium sp. WW5 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10175 and EX31_RS10180 are separated by 16 nucleotides EX31_RS10180 and EX31_RS10185 are separated by 71 nucleotides EX31_RS10185 and EX31_RS10190 overlap by 8 nucleotides
EX31_RS10175: EX31_RS10175 - amidohydrolase family protein, at 247,918 to 248,751
_RS10175
EX31_RS10180: EX31_RS10180 - type II 3-dehydroquinate dehydratase, at 248,768 to 249,208
_RS10180
EX31_RS10185: EX31_RS10185 - O-antigen ligase family protein, at 249,280 to 250,533
_RS10185
EX31_RS10190: EX31_RS10190 - glycosyltransferase, at 250,526 to 251,638
_RS10190
Position (kb) 249
250
251 Strain fitness (log2 ratio) -2
-1
0
1
2 at 248.554 kb on + strand, within EX31_RS10175 at 248.775 kb on - strand at 248.775 kb on - strand at 248.838 kb on - strand, within EX31_RS10180 at 249.138 kb on + strand, within EX31_RS10180 at 249.139 kb on - strand, within EX31_RS10180 at 249.171 kb on - strand at 249.582 kb on + strand, within EX31_RS10185 at 249.621 kb on + strand, within EX31_RS10185 at 249.630 kb on + strand, within EX31_RS10185 at 249.648 kb on - strand, within EX31_RS10185 at 249.650 kb on - strand, within EX31_RS10185 at 249.651 kb on + strand, within EX31_RS10185 at 249.652 kb on - strand, within EX31_RS10185 at 249.652 kb on - strand, within EX31_RS10185 at 249.707 kb on + strand, within EX31_RS10185 at 249.838 kb on - strand, within EX31_RS10185 at 249.844 kb on - strand, within EX31_RS10185 at 250.004 kb on + strand, within EX31_RS10185 at 250.119 kb on + strand, within EX31_RS10185 at 250.124 kb on - strand, within EX31_RS10185 at 250.211 kb on - strand, within EX31_RS10185 at 250.264 kb on - strand, within EX31_RS10185 at 250.351 kb on - strand, within EX31_RS10185 at 250.375 kb on - strand, within EX31_RS10185 at 250.582 kb on - strand at 250.582 kb on - strand at 250.582 kb on - strand at 250.712 kb on - strand, within EX31_RS10190 at 250.767 kb on + strand, within EX31_RS10190 at 250.768 kb on - strand, within EX31_RS10190 at 250.867 kb on - strand, within EX31_RS10190 at 250.933 kb on - strand, within EX31_RS10190 at 250.976 kb on + strand, within EX31_RS10190 at 250.977 kb on - strand, within EX31_RS10190 at 250.977 kb on - strand, within EX31_RS10190 at 251.217 kb on + strand, within EX31_RS10190
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. WW5 2:1 starting OD to Azospirillum remove 248,554 + EX31_RS10175 0.76 +0.1 248,775 - +0.9 248,775 - -1.1 248,838 - EX31_RS10180 0.16 +0.4 249,138 + EX31_RS10180 0.84 +0.7 249,139 - EX31_RS10180 0.84 +2.0 249,171 - -0.5 249,582 + EX31_RS10185 0.24 +0.9 249,621 + EX31_RS10185 0.27 -0.7 249,630 + EX31_RS10185 0.28 +1.1 249,648 - EX31_RS10185 0.29 +1.6 249,650 - EX31_RS10185 0.30 +1.7 249,651 + EX31_RS10185 0.30 +2.2 249,652 - EX31_RS10185 0.30 -0.5 249,652 - EX31_RS10185 0.30 +1.2 249,707 + EX31_RS10185 0.34 -1.9 249,838 - EX31_RS10185 0.44 +0.4 249,844 - EX31_RS10185 0.45 +0.3 250,004 + EX31_RS10185 0.58 -0.7 250,119 + EX31_RS10185 0.67 +1.0 250,124 - EX31_RS10185 0.67 +1.3 250,211 - EX31_RS10185 0.74 +0.0 250,264 - EX31_RS10185 0.78 +0.6 250,351 - EX31_RS10185 0.85 +0.4 250,375 - EX31_RS10185 0.87 +0.6 250,582 - -0.1 250,582 - -0.1 250,582 - -0.9 250,712 - EX31_RS10190 0.17 -0.5 250,767 + EX31_RS10190 0.22 +0.2 250,768 - EX31_RS10190 0.22 +0.6 250,867 - EX31_RS10190 0.31 -1.1 250,933 - EX31_RS10190 0.37 +0.1 250,976 + EX31_RS10190 0.40 +0.3 250,977 - EX31_RS10190 0.41 -0.9 250,977 - EX31_RS10190 0.41 -0.4 251,217 + EX31_RS10190 0.62 -0.1
Or see this region's nucleotide sequence