Experiment: NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19890 and EX31_RS19895 are separated by 30 nucleotides EX31_RS19895 and EX31_RS19900 are separated by 361 nucleotides EX31_RS19900 and EX31_RS19905 are separated by 147 nucleotides EX31_RS19905 and EX31_RS19910 overlap by 4 nucleotides
EX31_RS19890: EX31_RS19890 - hydrogenase nickel incorporation protein HypB, at 608,015 to 608,794
_RS19890
EX31_RS19895: EX31_RS19895 - hydrogenase maturation nickel metallochaperone HypA, at 608,825 to 609,169
_RS19895
EX31_RS19900: EX31_RS19900 - formate hydrogenlyase regulator HycA, at 609,531 to 609,998
_RS19900
EX31_RS19905: EX31_RS19905 - 4Fe-4S dicluster domain-containing protein, at 610,146 to 610,775
_RS19905
EX31_RS19910: EX31_RS19910 - NADH dehydrogenase, at 610,772 to 612,676
_RS19910
Position (kb) 609
610 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 608.540 kb on - strand, within EX31_RS19890 at 608.540 kb on - strand, within EX31_RS19890 at 608.618 kb on - strand, within EX31_RS19890 at 608.618 kb on - strand, within EX31_RS19890 at 608.623 kb on + strand, within EX31_RS19890 at 608.623 kb on + strand, within EX31_RS19890 at 608.624 kb on - strand, within EX31_RS19890 at 608.785 kb on + strand at 608.794 kb on + strand at 608.794 kb on + strand at 608.794 kb on + strand at 608.794 kb on + strand at 608.794 kb on + strand at 608.794 kb on + strand at 608.794 kb on + strand at 608.794 kb on + strand at 608.794 kb on + strand at 608.795 kb on - strand at 608.795 kb on - strand at 608.795 kb on - strand at 608.795 kb on - strand at 608.795 kb on - strand at 608.795 kb on - strand at 608.795 kb on - strand at 608.795 kb on - strand at 608.795 kb on - strand at 608.795 kb on - strand at 608.795 kb on - strand at 608.925 kb on - strand, within EX31_RS19895 at 608.925 kb on - strand, within EX31_RS19895 at 608.949 kb on + strand, within EX31_RS19895 at 608.950 kb on - strand, within EX31_RS19895 at 609.092 kb on - strand, within EX31_RS19895 at 609.106 kb on + strand, within EX31_RS19895 at 609.107 kb on - strand, within EX31_RS19895 at 609.157 kb on + strand at 609.170 kb on + strand at 609.171 kb on - strand at 609.171 kb on - strand at 609.194 kb on - strand at 609.196 kb on + strand at 609.216 kb on - strand at 609.216 kb on - strand at 609.216 kb on - strand at 609.219 kb on + strand at 609.220 kb on - strand at 609.226 kb on - strand at 609.226 kb on - strand at 609.282 kb on + strand at 609.303 kb on + strand at 609.303 kb on + strand at 609.303 kb on + strand at 609.303 kb on + strand at 609.303 kb on + strand at 609.303 kb on + strand at 609.303 kb on + strand at 609.303 kb on + strand at 609.304 kb on - strand at 609.304 kb on - strand at 609.304 kb on - strand at 609.304 kb on - strand at 609.304 kb on - strand at 609.304 kb on - strand at 609.304 kb on - strand at 609.304 kb on - strand at 609.304 kb on - strand at 609.305 kb on + strand at 609.305 kb on + strand at 609.305 kb on + strand at 609.305 kb on + strand at 609.305 kb on + strand at 609.305 kb on + strand at 609.305 kb on + strand at 609.305 kb on + strand at 609.305 kb on + strand at 609.306 kb on - strand at 609.306 kb on - strand at 609.306 kb on - strand at 609.306 kb on - strand at 609.306 kb on - strand at 609.316 kb on - strand at 609.316 kb on - strand at 609.337 kb on + strand at 609.337 kb on + strand at 609.338 kb on - strand at 609.338 kb on - strand at 609.338 kb on - strand at 609.432 kb on - strand at 609.447 kb on + strand at 609.447 kb on + strand at 609.447 kb on + strand at 609.447 kb on + strand at 609.447 kb on + strand at 609.447 kb on + strand at 609.447 kb on + strand at 609.448 kb on - strand at 609.448 kb on - strand at 609.448 kb on - strand at 609.448 kb on - strand at 609.448 kb on - strand at 609.448 kb on - strand at 609.456 kb on + strand at 609.479 kb on + strand at 609.502 kb on + strand at 609.502 kb on + strand at 609.502 kb on + strand at 609.502 kb on + strand at 609.503 kb on - strand at 609.503 kb on - strand at 609.503 kb on - strand at 609.503 kb on - strand at 609.557 kb on + strand at 609.568 kb on - strand at 609.568 kb on - strand at 609.569 kb on + strand at 609.570 kb on - strand at 609.571 kb on + strand at 609.572 kb on - strand at 609.572 kb on - strand at 609.729 kb on - strand, within EX31_RS19900 at 609.729 kb on - strand, within EX31_RS19900 at 609.750 kb on + strand, within EX31_RS19900 at 609.751 kb on - strand, within EX31_RS19900 at 609.867 kb on - strand, within EX31_RS19900 at 609.869 kb on + strand, within EX31_RS19900 at 609.875 kb on + strand, within EX31_RS19900 at 609.875 kb on + strand, within EX31_RS19900 at 609.875 kb on + strand, within EX31_RS19900 at 609.875 kb on + strand, within EX31_RS19900 at 609.876 kb on - strand, within EX31_RS19900 at 609.897 kb on + strand, within EX31_RS19900 at 609.898 kb on - strand, within EX31_RS19900 at 609.898 kb on - strand, within EX31_RS19900 at 609.898 kb on - strand, within EX31_RS19900 at 609.898 kb on - strand, within EX31_RS19900 at 609.918 kb on - strand, within EX31_RS19900 at 609.922 kb on - strand, within EX31_RS19900 at 609.922 kb on - strand, within EX31_RS19900 at 609.922 kb on - strand, within EX31_RS19900 at 609.951 kb on - strand, within EX31_RS19900 at 610.001 kb on + strand at 610.001 kb on + strand at 610.001 kb on + strand at 610.002 kb on - strand at 610.002 kb on - strand at 610.002 kb on - strand at 610.013 kb on + strand at 610.014 kb on - strand at 610.014 kb on - strand at 610.014 kb on - strand at 610.014 kb on - strand at 610.031 kb on + strand at 610.072 kb on - strand at 610.082 kb on - strand at 610.082 kb on - strand at 610.082 kb on - strand at 610.101 kb on + strand at 610.101 kb on + strand at 610.102 kb on - strand at 610.102 kb on - strand at 610.102 kb on - strand at 610.102 kb on - strand at 610.131 kb on + strand at 610.132 kb on - strand at 610.132 kb on - strand at 610.132 kb on - strand at 610.173 kb on + strand at 610.173 kb on + strand at 610.173 kb on + strand at 610.173 kb on + strand at 610.173 kb on + strand at 610.174 kb on - strand at 610.180 kb on + strand at 610.200 kb on - strand at 610.200 kb on - strand at 610.211 kb on + strand, within EX31_RS19905 at 610.211 kb on + strand, within EX31_RS19905 at 610.211 kb on + strand, within EX31_RS19905 at 610.212 kb on - strand, within EX31_RS19905 at 610.255 kb on + strand, within EX31_RS19905 at 610.364 kb on + strand, within EX31_RS19905 at 610.364 kb on + strand, within EX31_RS19905 at 610.496 kb on + strand, within EX31_RS19905 at 610.496 kb on + strand, within EX31_RS19905 at 610.497 kb on - strand, within EX31_RS19905 at 610.497 kb on - strand, within EX31_RS19905 at 610.497 kb on - strand, within EX31_RS19905 at 610.597 kb on + strand, within EX31_RS19905 at 610.720 kb on + strand at 610.720 kb on + strand at 610.721 kb on - strand at 610.721 kb on - strand at 610.746 kb on - strand at 610.782 kb on + strand at 610.782 kb on + strand at 610.782 kb on + strand at 610.783 kb on - strand at 610.786 kb on - strand at 610.846 kb on + strand at 610.846 kb on + strand at 610.847 kb on - strand at 610.858 kb on - strand at 610.878 kb on + strand at 610.879 kb on - strand at 610.879 kb on - strand at 610.879 kb on - strand at 610.930 kb on + strand at 610.930 kb on + strand at 610.930 kb on + strand at 610.931 kb on - strand at 610.931 kb on - strand at 610.950 kb on + strand at 610.951 kb on - strand at 610.975 kb on + strand, within EX31_RS19910 at 610.975 kb on + strand, within EX31_RS19910 at 610.975 kb on + strand, within EX31_RS19910 at 610.975 kb on + strand, within EX31_RS19910 at 610.975 kb on + strand, within EX31_RS19910 at 610.976 kb on - strand, within EX31_RS19910
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum remove 608,540 - EX31_RS19890 0.67 -0.6 608,540 - EX31_RS19890 0.67 -0.8 608,618 - EX31_RS19890 0.77 -0.1 608,618 - EX31_RS19890 0.77 -0.1 608,623 + EX31_RS19890 0.78 +0.9 608,623 + EX31_RS19890 0.78 -0.8 608,624 - EX31_RS19890 0.78 +0.6 608,785 + +0.6 608,794 + +0.2 608,794 + +1.4 608,794 + -2.5 608,794 + +0.5 608,794 + -0.7 608,794 + +1.3 608,794 + -0.8 608,794 + -1.4 608,794 + +1.9 608,795 - +0.5 608,795 - +0.7 608,795 - -0.7 608,795 - +1.2 608,795 - -0.2 608,795 - +0.2 608,795 - +0.6 608,795 - -0.1 608,795 - +1.0 608,795 - -0.9 608,795 - -0.6 608,925 - EX31_RS19895 0.29 -0.0 608,925 - EX31_RS19895 0.29 -1.0 608,949 + EX31_RS19895 0.36 +0.0 608,950 - EX31_RS19895 0.36 -0.0 609,092 - EX31_RS19895 0.77 -0.3 609,106 + EX31_RS19895 0.81 -0.3 609,107 - EX31_RS19895 0.82 -0.3 609,157 + +0.2 609,170 + -0.6 609,171 - +0.3 609,171 - +0.6 609,194 - +0.1 609,196 + +0.0 609,216 - -2.8 609,216 - -0.3 609,216 - +0.1 609,219 + -0.1 609,220 - -0.7 609,226 - -0.2 609,226 - -0.0 609,282 + +0.2 609,303 + +1.3 609,303 + +1.0 609,303 + +0.7 609,303 + +0.4 609,303 + -0.3 609,303 + -0.7 609,303 + +0.4 609,303 + -0.6 609,304 - -0.7 609,304 - -0.6 609,304 - +0.2 609,304 - -0.0 609,304 - +0.1 609,304 - -1.1 609,304 - +0.4 609,304 - -1.1 609,304 - +0.5 609,305 + +0.7 609,305 + +0.5 609,305 + +0.4 609,305 + +0.4 609,305 + +0.1 609,305 + +0.3 609,305 + +1.6 609,305 + +0.5 609,305 + +0.5 609,306 - -0.3 609,306 - +0.3 609,306 - -0.7 609,306 - +0.1 609,306 - -0.2 609,316 - -0.3 609,316 - +0.2 609,337 + +0.3 609,337 + -0.1 609,338 - +0.6 609,338 - +0.7 609,338 - -0.2 609,432 - -0.3 609,447 + -0.3 609,447 + -0.2 609,447 + +0.8 609,447 + +1.3 609,447 + +0.3 609,447 + -0.3 609,447 + +0.7 609,448 - +0.3 609,448 - -0.1 609,448 - -0.8 609,448 - +0.2 609,448 - -0.8 609,448 - -1.0 609,456 + -0.9 609,479 + -1.0 609,502 + -0.1 609,502 + +0.2 609,502 + +0.0 609,502 + -0.3 609,503 - -0.4 609,503 - +0.8 609,503 - -0.1 609,503 - +0.4 609,557 + +1.1 609,568 - +0.0 609,568 - +0.2 609,569 + +0.2 609,570 - -0.2 609,571 + -1.0 609,572 - +0.5 609,572 - +0.7 609,729 - EX31_RS19900 0.42 -0.6 609,729 - EX31_RS19900 0.42 -0.8 609,750 + EX31_RS19900 0.47 -2.3 609,751 - EX31_RS19900 0.47 -0.2 609,867 - EX31_RS19900 0.72 -0.7 609,869 + EX31_RS19900 0.72 +0.2 609,875 + EX31_RS19900 0.74 +0.6 609,875 + EX31_RS19900 0.74 -0.8 609,875 + EX31_RS19900 0.74 -0.8 609,875 + EX31_RS19900 0.74 +0.9 609,876 - EX31_RS19900 0.74 +0.3 609,897 + EX31_RS19900 0.78 +0.8 609,898 - EX31_RS19900 0.78 +0.4 609,898 - EX31_RS19900 0.78 +0.4 609,898 - EX31_RS19900 0.78 -1.5 609,898 - EX31_RS19900 0.78 -2.0 609,918 - EX31_RS19900 0.83 -0.7 609,922 - EX31_RS19900 0.84 -0.3 609,922 - EX31_RS19900 0.84 +0.2 609,922 - EX31_RS19900 0.84 -1.8 609,951 - EX31_RS19900 0.90 +0.3 610,001 + +0.9 610,001 + +0.4 610,001 + -0.5 610,002 - +0.6 610,002 - +0.2 610,002 - +0.3 610,013 + +0.2 610,014 - +0.5 610,014 - -0.3 610,014 - -0.8 610,014 - -0.7 610,031 + -1.2 610,072 - +0.5 610,082 - +0.5 610,082 - +0.3 610,082 - -1.2 610,101 + -0.3 610,101 + +0.5 610,102 - -0.7 610,102 - +1.0 610,102 - +1.0 610,102 - +0.3 610,131 + -1.0 610,132 - -1.3 610,132 - +0.2 610,132 - +1.9 610,173 + +0.3 610,173 + +0.9 610,173 + -0.1 610,173 + -0.2 610,173 + +0.0 610,174 - +0.1 610,180 + -1.0 610,200 - -0.0 610,200 - +0.8 610,211 + EX31_RS19905 0.10 -1.2 610,211 + EX31_RS19905 0.10 +0.1 610,211 + EX31_RS19905 0.10 +0.6 610,212 - EX31_RS19905 0.10 +0.9 610,255 + EX31_RS19905 0.17 -1.0 610,364 + EX31_RS19905 0.35 -0.1 610,364 + EX31_RS19905 0.35 -0.2 610,496 + EX31_RS19905 0.56 +0.7 610,496 + EX31_RS19905 0.56 -0.9 610,497 - EX31_RS19905 0.56 -0.9 610,497 - EX31_RS19905 0.56 -1.1 610,497 - EX31_RS19905 0.56 -0.4 610,597 + EX31_RS19905 0.72 +0.3 610,720 + +0.9 610,720 + +0.5 610,721 - +0.1 610,721 - -0.5 610,746 - +1.0 610,782 + -0.2 610,782 + +0.9 610,782 + -0.5 610,783 - -0.1 610,786 - -0.3 610,846 + -0.9 610,846 + +0.3 610,847 - -0.9 610,858 - +0.1 610,878 + -0.0 610,879 - -2.8 610,879 - +0.4 610,879 - +0.6 610,930 + -0.0 610,930 + +0.6 610,930 + +0.7 610,931 - -0.5 610,931 - +1.1 610,950 + -0.3 610,951 - -0.1 610,975 + EX31_RS19910 0.11 -0.2 610,975 + EX31_RS19910 0.11 +0.5 610,975 + EX31_RS19910 0.11 +1.3 610,975 + EX31_RS19910 0.11 -0.1 610,975 + EX31_RS19910 0.11 -0.1 610,976 - EX31_RS19910 0.11 -0.8
Or see this region's nucleotide sequence