Experiment: NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23725 and EX31_RS23730 are separated by 40 nucleotides EX31_RS23730 and EX31_RS23735 are separated by 64 nucleotides EX31_RS23735 and EX31_RS23740 are separated by 409 nucleotides
EX31_RS23725: EX31_RS23725 - DUF2132 domain-containing protein, at 511,637 to 512,005
_RS23725
EX31_RS23730: EX31_RS23730 - isochorismatase family protein, at 512,046 to 512,570
_RS23730
EX31_RS23735: EX31_RS23735 - uridine diphosphate-N-acetylglucosamine-binding protein YvcK, at 512,635 to 513,543
_RS23735
EX31_RS23740: EX31_RS23740 - GTP 3',8-cyclase MoaA, at 513,953 to 514,939
_RS23740
Position (kb)
512
513
514 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 511.645 kb on + strand at 511.645 kb on + strand at 511.645 kb on + strand at 511.645 kb on + strand at 511.646 kb on - strand at 511.646 kb on - strand at 511.723 kb on + strand, within EX31_RS23725 at 511.759 kb on + strand, within EX31_RS23725 at 511.760 kb on - strand, within EX31_RS23725 at 511.832 kb on - strand, within EX31_RS23725 at 511.850 kb on + strand, within EX31_RS23725 at 512.066 kb on + strand at 512.066 kb on + strand at 512.079 kb on - strand at 512.110 kb on - strand, within EX31_RS23730 at 512.224 kb on + strand, within EX31_RS23730 at 512.224 kb on + strand, within EX31_RS23730 at 512.224 kb on + strand, within EX31_RS23730 at 512.225 kb on - strand, within EX31_RS23730 at 512.225 kb on - strand, within EX31_RS23730 at 512.225 kb on - strand, within EX31_RS23730 at 512.236 kb on + strand, within EX31_RS23730 at 512.236 kb on + strand, within EX31_RS23730 at 512.236 kb on + strand, within EX31_RS23730 at 512.267 kb on + strand, within EX31_RS23730 at 512.290 kb on - strand, within EX31_RS23730 at 512.339 kb on + strand, within EX31_RS23730 at 512.339 kb on + strand, within EX31_RS23730 at 512.372 kb on - strand, within EX31_RS23730 at 512.406 kb on + strand, within EX31_RS23730 at 512.406 kb on + strand, within EX31_RS23730 at 512.406 kb on + strand, within EX31_RS23730 at 512.439 kb on - strand, within EX31_RS23730 at 512.466 kb on + strand, within EX31_RS23730 at 512.467 kb on - strand, within EX31_RS23730 at 512.486 kb on + strand, within EX31_RS23730 at 512.487 kb on - strand, within EX31_RS23730 at 512.506 kb on + strand, within EX31_RS23730 at 512.507 kb on - strand, within EX31_RS23730 at 512.616 kb on + strand at 512.616 kb on + strand at 512.616 kb on + strand at 512.617 kb on - strand at 512.617 kb on - strand at 512.617 kb on - strand at 512.617 kb on - strand at 512.618 kb on + strand at 512.630 kb on - strand at 512.821 kb on - strand, within EX31_RS23735 at 512.888 kb on + strand, within EX31_RS23735 at 512.888 kb on + strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.889 kb on - strand, within EX31_RS23735 at 512.895 kb on + strand, within EX31_RS23735 at 513.526 kb on + strand at 513.527 kb on - strand at 513.527 kb on - strand at 513.527 kb on - strand at 513.527 kb on - strand at 513.527 kb on - strand at 513.527 kb on - strand at 513.527 kb on - strand at 513.527 kb on - strand at 513.527 kb on - strand at 513.558 kb on - strand at 513.559 kb on + strand at 513.559 kb on + strand at 513.559 kb on + strand at 513.560 kb on - strand at 513.560 kb on - strand at 513.576 kb on - strand at 513.576 kb on - strand at 513.576 kb on - strand at 513.576 kb on - strand at 513.600 kb on - strand at 513.649 kb on + strand at 513.650 kb on - strand at 513.759 kb on + strand at 513.759 kb on + strand at 513.760 kb on - strand at 513.801 kb on - strand at 513.836 kb on + strand at 513.836 kb on + strand at 513.836 kb on + strand at 513.836 kb on + strand at 513.837 kb on - strand at 513.837 kb on - strand at 513.837 kb on - strand at 513.872 kb on + strand at 513.873 kb on - strand at 513.873 kb on - strand at 513.949 kb on + strand at 513.964 kb on + strand at 513.964 kb on + strand at 513.964 kb on + strand at 513.965 kb on - strand at 513.965 kb on - strand at 513.992 kb on + strand at 513.993 kb on - strand at 513.993 kb on - strand at 514.037 kb on + strand at 514.037 kb on + strand at 514.037 kb on + strand at 514.037 kb on + strand at 514.037 kb on + strand at 514.037 kb on + strand at 514.037 kb on + strand at 514.038 kb on - strand at 514.038 kb on - strand at 514.038 kb on - strand at 514.038 kb on - strand at 514.038 kb on - strand at 514.038 kb on - strand at 514.038 kb on - strand at 514.038 kb on - strand at 514.055 kb on + strand, within EX31_RS23740 at 514.055 kb on + strand, within EX31_RS23740 at 514.056 kb on - strand, within EX31_RS23740 at 514.056 kb on - strand, within EX31_RS23740 at 514.056 kb on - strand, within EX31_RS23740 at 514.078 kb on + strand, within EX31_RS23740 at 514.078 kb on + strand, within EX31_RS23740 at 514.078 kb on + strand, within EX31_RS23740 at 514.078 kb on + strand, within EX31_RS23740 at 514.079 kb on - strand, within EX31_RS23740 at 514.079 kb on - strand, within EX31_RS23740 at 514.079 kb on - strand, within EX31_RS23740 at 514.079 kb on - strand, within EX31_RS23740 at 514.392 kb on + strand, within EX31_RS23740 at 514.392 kb on + strand, within EX31_RS23740 at 514.392 kb on + strand, within EX31_RS23740 at 514.392 kb on + strand, within EX31_RS23740 at 514.392 kb on + strand, within EX31_RS23740 at 514.392 kb on + strand, within EX31_RS23740 at 514.392 kb on + strand, within EX31_RS23740 at 514.393 kb on - strand, within EX31_RS23740 at 514.393 kb on - strand, within EX31_RS23740 at 514.393 kb on - strand, within EX31_RS23740 at 514.393 kb on - strand, within EX31_RS23740 at 514.393 kb on - strand, within EX31_RS23740 at 514.393 kb on - strand, within EX31_RS23740 at 514.393 kb on - strand, within EX31_RS23740 at 514.393 kb on - strand, within EX31_RS23740 at 514.393 kb on - strand, within EX31_RS23740 at 514.393 kb on - strand, within EX31_RS23740 at 514.474 kb on + strand, within EX31_RS23740 at 514.474 kb on + strand, within EX31_RS23740 at 514.474 kb on + strand, within EX31_RS23740 at 514.475 kb on - strand, within EX31_RS23740 at 514.475 kb on - strand, within EX31_RS23740 at 514.475 kb on - strand, within EX31_RS23740
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum remove 511,645 + -3.2 511,645 + +0.5 511,645 + -0.7 511,645 + +0.7 511,646 - -1.2 511,646 - +0.6 511,723 + EX31_RS23725 0.23 -0.5 511,759 + EX31_RS23725 0.33 +0.2 511,760 - EX31_RS23725 0.33 -0.5 511,832 - EX31_RS23725 0.53 -0.0 511,850 + EX31_RS23725 0.58 -0.7 512,066 + +0.6 512,066 + -0.7 512,079 - +0.1 512,110 - EX31_RS23730 0.12 +0.2 512,224 + EX31_RS23730 0.34 -0.5 512,224 + EX31_RS23730 0.34 +0.1 512,224 + EX31_RS23730 0.34 +0.1 512,225 - EX31_RS23730 0.34 -0.6 512,225 - EX31_RS23730 0.34 -0.6 512,225 - EX31_RS23730 0.34 +0.8 512,236 + EX31_RS23730 0.36 +0.7 512,236 + EX31_RS23730 0.36 -1.4 512,236 + EX31_RS23730 0.36 +0.6 512,267 + EX31_RS23730 0.42 +0.9 512,290 - EX31_RS23730 0.46 +0.1 512,339 + EX31_RS23730 0.56 +0.9 512,339 + EX31_RS23730 0.56 +0.7 512,372 - EX31_RS23730 0.62 +1.5 512,406 + EX31_RS23730 0.69 +0.1 512,406 + EX31_RS23730 0.69 +0.0 512,406 + EX31_RS23730 0.69 +0.4 512,439 - EX31_RS23730 0.75 -0.3 512,466 + EX31_RS23730 0.80 +0.2 512,467 - EX31_RS23730 0.80 -0.3 512,486 + EX31_RS23730 0.84 -1.6 512,487 - EX31_RS23730 0.84 +0.2 512,506 + EX31_RS23730 0.88 -0.2 512,507 - EX31_RS23730 0.88 +1.0 512,616 + -0.5 512,616 + -0.9 512,616 + -2.0 512,617 - -0.1 512,617 - -0.2 512,617 - -0.6 512,617 - -0.1 512,618 + -0.6 512,630 - +0.1 512,821 - EX31_RS23735 0.20 -0.2 512,888 + EX31_RS23735 0.28 -0.0 512,888 + EX31_RS23735 0.28 -0.1 512,889 - EX31_RS23735 0.28 +1.4 512,889 - EX31_RS23735 0.28 +0.5 512,889 - EX31_RS23735 0.28 -1.4 512,889 - EX31_RS23735 0.28 -0.9 512,889 - EX31_RS23735 0.28 +0.8 512,889 - EX31_RS23735 0.28 -0.4 512,889 - EX31_RS23735 0.28 -0.0 512,889 - EX31_RS23735 0.28 -0.9 512,889 - EX31_RS23735 0.28 -1.3 512,889 - EX31_RS23735 0.28 -1.5 512,889 - EX31_RS23735 0.28 -1.8 512,889 - EX31_RS23735 0.28 -1.6 512,889 - EX31_RS23735 0.28 -0.4 512,895 + EX31_RS23735 0.29 -2.2 513,526 + -0.3 513,527 - +1.2 513,527 - +1.8 513,527 - +0.3 513,527 - +0.3 513,527 - +0.4 513,527 - +0.7 513,527 - -0.5 513,527 - -0.6 513,527 - -1.0 513,558 - +0.5 513,559 + -0.3 513,559 + -0.3 513,559 + -0.8 513,560 - +0.9 513,560 - -0.2 513,576 - +0.4 513,576 - -0.1 513,576 - -0.4 513,576 - +0.2 513,600 - -1.0 513,649 + -0.3 513,650 - +0.6 513,759 + +0.1 513,759 + -1.0 513,760 - +0.1 513,801 - -1.8 513,836 + -0.1 513,836 + +0.1 513,836 + -0.7 513,836 + +0.1 513,837 - +0.3 513,837 - +0.0 513,837 - -0.2 513,872 + +0.5 513,873 - +0.6 513,873 - +0.1 513,949 + +0.1 513,964 + -0.4 513,964 + -0.1 513,964 + -0.5 513,965 - +0.0 513,965 - +0.3 513,992 + +0.3 513,993 - +0.7 513,993 - -0.5 514,037 + +0.4 514,037 + +0.2 514,037 + -0.1 514,037 + +0.1 514,037 + -1.6 514,037 + +0.1 514,037 + +0.5 514,038 - -1.1 514,038 - +0.0 514,038 - -1.0 514,038 - +0.4 514,038 - -0.7 514,038 - +0.5 514,038 - +0.9 514,038 - -0.6 514,055 + EX31_RS23740 0.10 -0.1 514,055 + EX31_RS23740 0.10 +0.2 514,056 - EX31_RS23740 0.10 +0.4 514,056 - EX31_RS23740 0.10 -1.1 514,056 - EX31_RS23740 0.10 -0.2 514,078 + EX31_RS23740 0.13 -0.3 514,078 + EX31_RS23740 0.13 +0.2 514,078 + EX31_RS23740 0.13 -0.6 514,078 + EX31_RS23740 0.13 +1.3 514,079 - EX31_RS23740 0.13 -1.0 514,079 - EX31_RS23740 0.13 +0.8 514,079 - EX31_RS23740 0.13 +0.6 514,079 - EX31_RS23740 0.13 -0.1 514,392 + EX31_RS23740 0.44 +0.4 514,392 + EX31_RS23740 0.44 +0.7 514,392 + EX31_RS23740 0.44 +0.8 514,392 + EX31_RS23740 0.44 -0.1 514,392 + EX31_RS23740 0.44 +0.2 514,392 + EX31_RS23740 0.44 +0.4 514,392 + EX31_RS23740 0.44 +0.2 514,393 - EX31_RS23740 0.45 +0.8 514,393 - EX31_RS23740 0.45 +0.1 514,393 - EX31_RS23740 0.45 +1.7 514,393 - EX31_RS23740 0.45 +0.3 514,393 - EX31_RS23740 0.45 +0.0 514,393 - EX31_RS23740 0.45 +0.1 514,393 - EX31_RS23740 0.45 +2.0 514,393 - EX31_RS23740 0.45 +0.0 514,393 - EX31_RS23740 0.45 +0.4 514,393 - EX31_RS23740 0.45 +0.4 514,474 + EX31_RS23740 0.53 +0.6 514,474 + EX31_RS23740 0.53 +0.9 514,474 + EX31_RS23740 0.53 -0.9 514,475 - EX31_RS23740 0.53 +0.4 514,475 - EX31_RS23740 0.53 +0.7 514,475 - EX31_RS23740 0.53 +0.6
Or see this region's nucleotide sequence