Experiment: NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19165 and EX31_RS19170 are separated by 61 nucleotides EX31_RS19170 and EX31_RS19175 are separated by 8 nucleotides EX31_RS19175 and EX31_RS19180 are separated by 205 nucleotides
EX31_RS19165: EX31_RS19165 - cytochrome c, at 428,102 to 429,505
_RS19165
EX31_RS19170: EX31_RS19170 - SDR family oxidoreductase, at 429,567 to 430,310
_RS19170
EX31_RS19175: EX31_RS19175 - gluconate 5-dehydrogenase, at 430,319 to 431,083
_RS19175
EX31_RS19180: EX31_RS19180 - LacI family DNA-binding transcriptional regulator, at 431,289 to 432,272
_RS19180
Position (kb)
430
431
432 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 429.396 kb on + strand at 429.396 kb on + strand at 429.396 kb on + strand at 429.504 kb on - strand at 429.504 kb on - strand at 429.504 kb on - strand at 429.511 kb on - strand at 429.550 kb on - strand at 429.550 kb on - strand at 429.568 kb on + strand at 429.569 kb on - strand at 429.603 kb on + strand at 429.604 kb on - strand at 429.624 kb on - strand at 429.647 kb on + strand, within EX31_RS19170 at 429.701 kb on + strand, within EX31_RS19170 at 429.701 kb on + strand, within EX31_RS19170 at 429.737 kb on + strand, within EX31_RS19170 at 429.857 kb on - strand, within EX31_RS19170 at 429.857 kb on - strand, within EX31_RS19170 at 429.857 kb on - strand, within EX31_RS19170 at 429.911 kb on + strand, within EX31_RS19170 at 429.911 kb on + strand, within EX31_RS19170 at 429.912 kb on - strand, within EX31_RS19170 at 429.912 kb on - strand, within EX31_RS19170 at 429.912 kb on - strand, within EX31_RS19170 at 429.912 kb on - strand, within EX31_RS19170 at 429.912 kb on - strand, within EX31_RS19170 at 429.989 kb on + strand, within EX31_RS19170 at 430.028 kb on - strand, within EX31_RS19170 at 430.050 kb on - strand, within EX31_RS19170 at 430.050 kb on - strand, within EX31_RS19170 at 430.062 kb on - strand, within EX31_RS19170 at 430.100 kb on + strand, within EX31_RS19170 at 430.172 kb on + strand, within EX31_RS19170 at 430.205 kb on + strand, within EX31_RS19170 at 430.209 kb on - strand, within EX31_RS19170 at 430.209 kb on - strand, within EX31_RS19170 at 430.230 kb on - strand, within EX31_RS19170 at 430.293 kb on + strand at 430.294 kb on - strand at 430.320 kb on + strand at 430.320 kb on + strand at 430.320 kb on + strand at 430.321 kb on - strand at 430.321 kb on - strand at 430.321 kb on - strand at 430.321 kb on - strand at 430.321 kb on - strand at 430.321 kb on - strand at 430.331 kb on + strand at 430.331 kb on + strand at 430.331 kb on + strand at 430.332 kb on - strand at 430.332 kb on - strand at 430.332 kb on - strand at 430.332 kb on - strand at 430.332 kb on - strand at 430.332 kb on - strand at 430.332 kb on - strand at 430.353 kb on - strand at 430.353 kb on - strand at 430.353 kb on - strand at 430.391 kb on + strand at 430.391 kb on + strand at 430.391 kb on + strand at 430.391 kb on + strand at 430.392 kb on - strand at 430.392 kb on - strand at 430.392 kb on - strand at 430.395 kb on + strand at 430.395 kb on + strand at 430.395 kb on + strand at 430.395 kb on + strand at 430.395 kb on + strand at 430.395 kb on + strand at 430.395 kb on + strand at 430.396 kb on - strand, within EX31_RS19175 at 430.396 kb on - strand, within EX31_RS19175 at 430.396 kb on - strand, within EX31_RS19175 at 430.396 kb on - strand, within EX31_RS19175 at 430.396 kb on - strand, within EX31_RS19175 at 430.396 kb on - strand, within EX31_RS19175 at 430.396 kb on - strand, within EX31_RS19175 at 430.396 kb on - strand, within EX31_RS19175 at 430.396 kb on - strand, within EX31_RS19175 at 430.396 kb on - strand, within EX31_RS19175 at 430.453 kb on + strand, within EX31_RS19175 at 430.453 kb on + strand, within EX31_RS19175 at 430.543 kb on + strand, within EX31_RS19175 at 430.543 kb on + strand, within EX31_RS19175 at 430.544 kb on - strand, within EX31_RS19175 at 430.548 kb on + strand, within EX31_RS19175 at 430.549 kb on - strand, within EX31_RS19175 at 430.549 kb on - strand, within EX31_RS19175 at 430.549 kb on - strand, within EX31_RS19175 at 430.693 kb on - strand, within EX31_RS19175 at 430.693 kb on - strand, within EX31_RS19175 at 430.693 kb on - strand, within EX31_RS19175 at 430.812 kb on - strand, within EX31_RS19175 at 430.846 kb on + strand, within EX31_RS19175 at 430.847 kb on - strand, within EX31_RS19175 at 430.975 kb on + strand, within EX31_RS19175 at 431.056 kb on + strand at 431.057 kb on - strand at 431.057 kb on - strand at 431.057 kb on - strand at 431.127 kb on + strand at 431.127 kb on + strand at 431.128 kb on - strand at 431.128 kb on - strand at 431.192 kb on + strand at 431.249 kb on + strand at 431.257 kb on + strand at 431.257 kb on + strand at 431.258 kb on - strand at 431.258 kb on - strand at 431.258 kb on - strand at 431.258 kb on - strand at 431.259 kb on + strand at 431.259 kb on + strand at 431.259 kb on + strand at 431.259 kb on + strand at 431.259 kb on + strand at 431.259 kb on + strand at 431.259 kb on + strand at 431.260 kb on - strand at 431.260 kb on - strand at 431.260 kb on - strand at 431.260 kb on - strand at 431.260 kb on - strand at 431.260 kb on - strand at 431.311 kb on + strand at 431.311 kb on + strand at 431.485 kb on + strand, within EX31_RS19180 at 431.485 kb on + strand, within EX31_RS19180 at 431.486 kb on - strand, within EX31_RS19180 at 431.572 kb on + strand, within EX31_RS19180 at 431.580 kb on + strand, within EX31_RS19180 at 431.580 kb on + strand, within EX31_RS19180 at 431.580 kb on + strand, within EX31_RS19180 at 431.581 kb on - strand, within EX31_RS19180 at 431.586 kb on + strand, within EX31_RS19180 at 431.586 kb on + strand, within EX31_RS19180 at 431.618 kb on + strand, within EX31_RS19180 at 431.618 kb on + strand, within EX31_RS19180 at 431.619 kb on - strand, within EX31_RS19180 at 431.619 kb on - strand, within EX31_RS19180 at 431.688 kb on + strand, within EX31_RS19180 at 431.798 kb on + strand, within EX31_RS19180 at 431.798 kb on + strand, within EX31_RS19180 at 431.799 kb on - strand, within EX31_RS19180 at 431.832 kb on + strand, within EX31_RS19180 at 431.880 kb on + strand, within EX31_RS19180 at 431.881 kb on - strand, within EX31_RS19180 at 431.921 kb on + strand, within EX31_RS19180 at 431.921 kb on + strand, within EX31_RS19180 at 431.921 kb on + strand, within EX31_RS19180 at 431.921 kb on + strand, within EX31_RS19180 at 431.922 kb on - strand, within EX31_RS19180 at 431.922 kb on - strand, within EX31_RS19180 at 431.930 kb on + strand, within EX31_RS19180 at 431.930 kb on + strand, within EX31_RS19180 at 431.933 kb on + strand, within EX31_RS19180 at 431.933 kb on + strand, within EX31_RS19180 at 431.933 kb on + strand, within EX31_RS19180 at 431.933 kb on + strand, within EX31_RS19180 at 431.933 kb on + strand, within EX31_RS19180 at 431.948 kb on - strand, within EX31_RS19180 at 431.951 kb on + strand, within EX31_RS19180 at 431.951 kb on + strand, within EX31_RS19180 at 431.951 kb on + strand, within EX31_RS19180 at 431.952 kb on - strand, within EX31_RS19180 at 431.986 kb on + strand, within EX31_RS19180 at 431.986 kb on + strand, within EX31_RS19180 at 431.986 kb on + strand, within EX31_RS19180 at 431.987 kb on - strand, within EX31_RS19180 at 431.987 kb on - strand, within EX31_RS19180 at 431.988 kb on + strand, within EX31_RS19180 at 432.043 kb on + strand, within EX31_RS19180 at 432.053 kb on + strand, within EX31_RS19180
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum remove 429,396 + -0.8 429,396 + +0.5 429,396 + -0.6 429,504 - -0.4 429,504 - +0.7 429,504 - -0.4 429,511 - -0.4 429,550 - -0.3 429,550 - -0.4 429,568 + +0.2 429,569 - -0.6 429,603 + +0.2 429,604 - -1.3 429,624 - +0.0 429,647 + EX31_RS19170 0.11 -1.7 429,701 + EX31_RS19170 0.18 +1.6 429,701 + EX31_RS19170 0.18 +0.1 429,737 + EX31_RS19170 0.23 +0.5 429,857 - EX31_RS19170 0.39 -0.7 429,857 - EX31_RS19170 0.39 +0.4 429,857 - EX31_RS19170 0.39 +0.9 429,911 + EX31_RS19170 0.46 +0.2 429,911 + EX31_RS19170 0.46 -1.8 429,912 - EX31_RS19170 0.46 -1.7 429,912 - EX31_RS19170 0.46 -1.2 429,912 - EX31_RS19170 0.46 +0.8 429,912 - EX31_RS19170 0.46 +1.0 429,912 - EX31_RS19170 0.46 +0.4 429,989 + EX31_RS19170 0.57 +0.8 430,028 - EX31_RS19170 0.62 -1.0 430,050 - EX31_RS19170 0.65 +1.2 430,050 - EX31_RS19170 0.65 -2.2 430,062 - EX31_RS19170 0.67 -1.1 430,100 + EX31_RS19170 0.72 +0.3 430,172 + EX31_RS19170 0.81 -1.0 430,205 + EX31_RS19170 0.86 -0.6 430,209 - EX31_RS19170 0.86 -0.1 430,209 - EX31_RS19170 0.86 +0.1 430,230 - EX31_RS19170 0.89 +0.9 430,293 + -1.3 430,294 - -1.1 430,320 + -1.1 430,320 + +2.1 430,320 + -1.7 430,321 - +0.4 430,321 - +0.5 430,321 - -1.8 430,321 - -0.1 430,321 - -0.8 430,321 - -0.6 430,331 + +0.0 430,331 + -0.3 430,331 + -1.6 430,332 - -0.9 430,332 - +0.1 430,332 - +0.1 430,332 - -0.0 430,332 - -1.7 430,332 - +0.7 430,332 - -0.2 430,353 - +0.9 430,353 - -0.2 430,353 - -2.2 430,391 + +0.8 430,391 + -0.4 430,391 + -0.0 430,391 + +1.0 430,392 - +1.4 430,392 - -2.9 430,392 - +0.1 430,395 + -0.1 430,395 + +1.0 430,395 + +1.4 430,395 + -0.1 430,395 + +0.3 430,395 + -0.3 430,395 + -0.8 430,396 - EX31_RS19175 0.10 -0.5 430,396 - EX31_RS19175 0.10 +0.6 430,396 - EX31_RS19175 0.10 +0.2 430,396 - EX31_RS19175 0.10 -0.2 430,396 - EX31_RS19175 0.10 -2.0 430,396 - EX31_RS19175 0.10 -0.4 430,396 - EX31_RS19175 0.10 -0.9 430,396 - EX31_RS19175 0.10 -1.8 430,396 - EX31_RS19175 0.10 +0.1 430,396 - EX31_RS19175 0.10 -1.5 430,453 + EX31_RS19175 0.18 -0.1 430,453 + EX31_RS19175 0.18 +0.2 430,543 + EX31_RS19175 0.29 -2.8 430,543 + EX31_RS19175 0.29 -1.9 430,544 - EX31_RS19175 0.29 -2.6 430,548 + EX31_RS19175 0.30 +0.2 430,549 - EX31_RS19175 0.30 +0.2 430,549 - EX31_RS19175 0.30 -0.4 430,549 - EX31_RS19175 0.30 -0.7 430,693 - EX31_RS19175 0.49 -0.5 430,693 - EX31_RS19175 0.49 -0.4 430,693 - EX31_RS19175 0.49 -1.4 430,812 - EX31_RS19175 0.64 -0.4 430,846 + EX31_RS19175 0.69 -0.6 430,847 - EX31_RS19175 0.69 +0.1 430,975 + EX31_RS19175 0.86 +0.2 431,056 + -0.4 431,057 - +1.3 431,057 - +0.5 431,057 - +1.9 431,127 + +1.0 431,127 + -0.3 431,128 - -0.3 431,128 - -0.1 431,192 + -0.6 431,249 + +0.4 431,257 + +0.4 431,257 + -1.7 431,258 - +0.9 431,258 - -0.3 431,258 - +0.5 431,258 - -1.6 431,259 + +0.1 431,259 + +1.4 431,259 + +0.2 431,259 + +1.0 431,259 + -1.1 431,259 + +0.7 431,259 + +1.2 431,260 - -2.1 431,260 - -0.6 431,260 - +0.7 431,260 - -0.0 431,260 - -0.5 431,260 - +0.6 431,311 + -1.4 431,311 + -1.2 431,485 + EX31_RS19180 0.20 +0.6 431,485 + EX31_RS19180 0.20 +0.7 431,486 - EX31_RS19180 0.20 -0.9 431,572 + EX31_RS19180 0.29 +0.3 431,580 + EX31_RS19180 0.30 -0.9 431,580 + EX31_RS19180 0.30 -0.4 431,580 + EX31_RS19180 0.30 -0.6 431,581 - EX31_RS19180 0.30 +2.3 431,586 + EX31_RS19180 0.30 -0.2 431,586 + EX31_RS19180 0.30 -0.1 431,618 + EX31_RS19180 0.33 -0.2 431,618 + EX31_RS19180 0.33 +0.4 431,619 - EX31_RS19180 0.34 +0.6 431,619 - EX31_RS19180 0.34 +0.9 431,688 + EX31_RS19180 0.41 +0.6 431,798 + EX31_RS19180 0.52 +0.6 431,798 + EX31_RS19180 0.52 -1.3 431,799 - EX31_RS19180 0.52 -2.0 431,832 + EX31_RS19180 0.55 +0.7 431,880 + EX31_RS19180 0.60 -0.9 431,881 - EX31_RS19180 0.60 -0.2 431,921 + EX31_RS19180 0.64 +0.1 431,921 + EX31_RS19180 0.64 -0.2 431,921 + EX31_RS19180 0.64 -0.5 431,921 + EX31_RS19180 0.64 +0.3 431,922 - EX31_RS19180 0.64 -1.2 431,922 - EX31_RS19180 0.64 -1.0 431,930 + EX31_RS19180 0.65 +1.2 431,930 + EX31_RS19180 0.65 -0.2 431,933 + EX31_RS19180 0.65 +0.1 431,933 + EX31_RS19180 0.65 +0.9 431,933 + EX31_RS19180 0.65 +1.0 431,933 + EX31_RS19180 0.65 -0.5 431,933 + EX31_RS19180 0.65 +0.7 431,948 - EX31_RS19180 0.67 +1.6 431,951 + EX31_RS19180 0.67 +1.5 431,951 + EX31_RS19180 0.67 -1.3 431,951 + EX31_RS19180 0.67 -0.4 431,952 - EX31_RS19180 0.67 -0.7 431,986 + EX31_RS19180 0.71 +0.3 431,986 + EX31_RS19180 0.71 -0.2 431,986 + EX31_RS19180 0.71 -2.6 431,987 - EX31_RS19180 0.71 +0.3 431,987 - EX31_RS19180 0.71 -0.6 431,988 + EX31_RS19180 0.71 -1.6 432,043 + EX31_RS19180 0.77 -0.3 432,053 + EX31_RS19180 0.78 -0.0
Or see this region's nucleotide sequence