Experiment: NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17580 and EX31_RS17585 overlap by 4 nucleotides EX31_RS17585 and EX31_RS17590 are separated by 137 nucleotides EX31_RS17590 and EX31_RS17595 are separated by 337 nucleotides EX31_RS17595 and EX31_RS17600 are separated by 4 nucleotides
EX31_RS17580: EX31_RS17580 - ABC transporter ATP-binding protein, at 70,350 to 71,045
_RS17580
EX31_RS17585: EX31_RS17585 - glutathione peroxidase, at 71,042 to 71,524
_RS17585
EX31_RS17590: EX31_RS17590 - thymidylate kinase, at 71,662 to 72,279
_RS17590
EX31_RS17595: EX31_RS17595 - chorismate mutase, at 72,617 to 72,913
_RS17595
EX31_RS17600: EX31_RS17600 - LysE family translocator, at 72,918 to 73,457
_RS17600
Position (kb) 71
72
73 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 70.740 kb on - strand, within EX31_RS17580 at 70.831 kb on + strand, within EX31_RS17580 at 70.831 kb on + strand, within EX31_RS17580 at 70.831 kb on + strand, within EX31_RS17580 at 70.832 kb on - strand, within EX31_RS17580 at 70.832 kb on - strand, within EX31_RS17580 at 70.832 kb on - strand, within EX31_RS17580 at 70.983 kb on + strand at 70.983 kb on + strand at 71.002 kb on - strand at 71.002 kb on - strand at 71.011 kb on + strand at 71.011 kb on + strand at 71.044 kb on - strand at 71.044 kb on - strand at 71.144 kb on - strand, within EX31_RS17585 at 71.144 kb on - strand, within EX31_RS17585 at 71.200 kb on - strand, within EX31_RS17585 at 71.200 kb on - strand, within EX31_RS17585 at 71.203 kb on + strand, within EX31_RS17585 at 71.203 kb on + strand, within EX31_RS17585 at 71.204 kb on - strand, within EX31_RS17585 at 71.219 kb on - strand, within EX31_RS17585 at 71.249 kb on + strand, within EX31_RS17585 at 71.268 kb on + strand, within EX31_RS17585 at 71.268 kb on + strand, within EX31_RS17585 at 71.269 kb on - strand, within EX31_RS17585 at 71.269 kb on - strand, within EX31_RS17585 at 71.371 kb on - strand, within EX31_RS17585 at 71.453 kb on - strand, within EX31_RS17585 at 71.461 kb on - strand, within EX31_RS17585 at 71.607 kb on + strand at 71.608 kb on - strand at 71.608 kb on - strand at 71.608 kb on - strand at 71.612 kb on + strand at 71.613 kb on - strand at 71.613 kb on - strand at 71.664 kb on - strand at 71.664 kb on - strand at 71.664 kb on - strand at 71.684 kb on + strand at 71.697 kb on + strand at 71.697 kb on + strand at 71.698 kb on - strand at 71.734 kb on - strand, within EX31_RS17590 at 71.741 kb on - strand, within EX31_RS17590 at 71.808 kb on + strand, within EX31_RS17590 at 71.809 kb on - strand, within EX31_RS17590 at 71.809 kb on - strand, within EX31_RS17590 at 71.809 kb on - strand, within EX31_RS17590 at 71.820 kb on + strand, within EX31_RS17590 at 71.821 kb on - strand, within EX31_RS17590 at 71.821 kb on - strand, within EX31_RS17590 at 71.823 kb on + strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.827 kb on + strand, within EX31_RS17590 at 71.828 kb on - strand, within EX31_RS17590 at 71.866 kb on - strand, within EX31_RS17590 at 71.866 kb on - strand, within EX31_RS17590 at 71.926 kb on - strand, within EX31_RS17590 at 71.926 kb on - strand, within EX31_RS17590 at 71.926 kb on - strand, within EX31_RS17590 at 72.000 kb on + strand, within EX31_RS17590 at 72.000 kb on + strand, within EX31_RS17590 at 72.000 kb on + strand, within EX31_RS17590 at 72.001 kb on - strand, within EX31_RS17590 at 72.001 kb on - strand, within EX31_RS17590 at 72.001 kb on - strand, within EX31_RS17590 at 72.001 kb on - strand, within EX31_RS17590 at 72.001 kb on - strand, within EX31_RS17590 at 72.144 kb on - strand, within EX31_RS17590 at 72.144 kb on - strand, within EX31_RS17590 at 72.261 kb on + strand at 72.262 kb on - strand at 72.262 kb on - strand at 72.299 kb on - strand at 72.299 kb on - strand at 72.299 kb on - strand at 72.338 kb on + strand at 72.338 kb on + strand at 72.339 kb on - strand at 72.339 kb on - strand at 72.339 kb on - strand at 72.414 kb on + strand at 72.414 kb on + strand at 72.414 kb on + strand at 72.414 kb on + strand at 72.433 kb on - strand at 72.503 kb on + strand at 72.504 kb on - strand at 72.510 kb on + strand at 72.511 kb on - strand at 72.515 kb on + strand at 72.515 kb on + strand at 72.515 kb on + strand at 72.515 kb on + strand at 72.516 kb on - strand at 72.516 kb on - strand at 72.516 kb on - strand at 72.516 kb on - strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.519 kb on + strand at 72.519 kb on + strand at 72.519 kb on + strand at 72.519 kb on + strand at 72.519 kb on + strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.558 kb on + strand at 72.559 kb on - strand at 72.578 kb on + strand at 72.578 kb on + strand at 72.579 kb on - strand at 72.579 kb on - strand at 72.579 kb on - strand at 72.644 kb on - strand at 72.679 kb on + strand, within EX31_RS17595 at 72.709 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.929 kb on + strand at 72.929 kb on + strand at 72.929 kb on + strand at 72.930 kb on - strand at 72.942 kb on - strand at 73.064 kb on + strand, within EX31_RS17600 at 73.077 kb on + strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.150 kb on + strand, within EX31_RS17600 at 73.196 kb on + strand, within EX31_RS17600 at 73.196 kb on + strand, within EX31_RS17600 at 73.258 kb on + strand, within EX31_RS17600 at 73.258 kb on + strand, within EX31_RS17600 at 73.258 kb on + strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum remove 70,740 - EX31_RS17580 0.56 -0.9 70,831 + EX31_RS17580 0.69 -1.4 70,831 + EX31_RS17580 0.69 -0.3 70,831 + EX31_RS17580 0.69 -1.4 70,832 - EX31_RS17580 0.69 +1.0 70,832 - EX31_RS17580 0.69 -1.7 70,832 - EX31_RS17580 0.69 -2.4 70,983 + -0.8 70,983 + -0.6 71,002 - -1.3 71,002 - +0.1 71,011 + -0.8 71,011 + +0.4 71,044 - -1.9 71,044 - +0.2 71,144 - EX31_RS17585 0.21 +0.7 71,144 - EX31_RS17585 0.21 +1.6 71,200 - EX31_RS17585 0.33 +0.4 71,200 - EX31_RS17585 0.33 +1.2 71,203 + EX31_RS17585 0.33 +0.3 71,203 + EX31_RS17585 0.33 -2.0 71,204 - EX31_RS17585 0.34 +0.7 71,219 - EX31_RS17585 0.37 -2.0 71,249 + EX31_RS17585 0.43 +0.1 71,268 + EX31_RS17585 0.47 +1.8 71,268 + EX31_RS17585 0.47 -0.1 71,269 - EX31_RS17585 0.47 +0.3 71,269 - EX31_RS17585 0.47 +0.6 71,371 - EX31_RS17585 0.68 -0.8 71,453 - EX31_RS17585 0.85 -0.5 71,461 - EX31_RS17585 0.87 -0.7 71,607 + -0.3 71,608 - -0.5 71,608 - -0.8 71,608 - -1.1 71,612 + -1.5 71,613 - +0.9 71,613 - -1.7 71,664 - +0.5 71,664 - +0.7 71,664 - +0.7 71,684 + -0.0 71,697 + +0.2 71,697 + +0.6 71,698 - +1.7 71,734 - EX31_RS17590 0.12 +0.4 71,741 - EX31_RS17590 0.13 -0.5 71,808 + EX31_RS17590 0.24 +0.2 71,809 - EX31_RS17590 0.24 -0.1 71,809 - EX31_RS17590 0.24 -2.3 71,809 - EX31_RS17590 0.24 +1.1 71,820 + EX31_RS17590 0.26 +0.5 71,821 - EX31_RS17590 0.26 -1.0 71,821 - EX31_RS17590 0.26 -3.3 71,823 + EX31_RS17590 0.26 -0.7 71,824 - EX31_RS17590 0.26 -0.6 71,824 - EX31_RS17590 0.26 +0.5 71,824 - EX31_RS17590 0.26 -0.4 71,824 - EX31_RS17590 0.26 -1.9 71,824 - EX31_RS17590 0.26 -0.1 71,824 - EX31_RS17590 0.26 -0.7 71,824 - EX31_RS17590 0.26 -0.2 71,824 - EX31_RS17590 0.26 +0.1 71,824 - EX31_RS17590 0.26 -0.7 71,827 + EX31_RS17590 0.27 +0.5 71,828 - EX31_RS17590 0.27 -1.2 71,866 - EX31_RS17590 0.33 -1.1 71,866 - EX31_RS17590 0.33 -1.4 71,926 - EX31_RS17590 0.43 -0.5 71,926 - EX31_RS17590 0.43 +0.9 71,926 - EX31_RS17590 0.43 +0.2 72,000 + EX31_RS17590 0.55 -0.6 72,000 + EX31_RS17590 0.55 +2.7 72,000 + EX31_RS17590 0.55 +0.1 72,001 - EX31_RS17590 0.55 +0.6 72,001 - EX31_RS17590 0.55 +0.7 72,001 - EX31_RS17590 0.55 -0.4 72,001 - EX31_RS17590 0.55 +0.6 72,001 - EX31_RS17590 0.55 +0.1 72,144 - EX31_RS17590 0.78 +0.4 72,144 - EX31_RS17590 0.78 +0.5 72,261 + +1.9 72,262 - +1.4 72,262 - +0.8 72,299 - -3.3 72,299 - -0.5 72,299 - -0.6 72,338 + +1.4 72,338 + +0.4 72,339 - +0.9 72,339 - +2.9 72,339 - -0.9 72,414 + +0.3 72,414 + +0.5 72,414 + +1.5 72,414 + +0.4 72,433 - +0.0 72,503 + -0.2 72,504 - -1.6 72,510 + -0.6 72,511 - -1.0 72,515 + +0.7 72,515 + -0.6 72,515 + +0.7 72,515 + +0.1 72,516 - +0.8 72,516 - -0.9 72,516 - +0.3 72,516 - +0.1 72,517 + +0.2 72,517 + +0.3 72,517 + -1.2 72,517 + +0.4 72,517 + -0.7 72,517 + +1.1 72,517 + -2.0 72,517 + +0.7 72,518 - -0.2 72,518 - -0.5 72,518 - -0.7 72,518 - -1.6 72,518 - -0.7 72,518 - +1.1 72,518 - -1.5 72,518 - -0.9 72,518 - -3.0 72,519 + +0.8 72,519 + +0.2 72,519 + +0.9 72,519 + +0.5 72,519 + -0.3 72,520 - -1.3 72,520 - +0.1 72,520 - +0.8 72,520 - -0.1 72,520 - -0.7 72,520 - -2.3 72,520 - -0.9 72,520 - -0.3 72,558 + -0.9 72,559 - +0.2 72,578 + -0.6 72,578 + -0.5 72,579 - +1.4 72,579 - +1.2 72,579 - -0.8 72,644 - +1.1 72,679 + EX31_RS17595 0.21 -0.3 72,709 + EX31_RS17595 0.31 +0.3 72,786 + EX31_RS17595 0.57 +1.3 72,786 + EX31_RS17595 0.57 +0.7 72,786 + EX31_RS17595 0.57 +0.6 72,786 + EX31_RS17595 0.57 -0.7 72,786 + EX31_RS17595 0.57 +0.1 72,786 + EX31_RS17595 0.57 +1.4 72,787 - EX31_RS17595 0.57 +1.5 72,787 - EX31_RS17595 0.57 +0.7 72,787 - EX31_RS17595 0.57 +1.5 72,787 - EX31_RS17595 0.57 +0.5 72,787 - EX31_RS17595 0.57 -1.3 72,787 - EX31_RS17595 0.57 +0.6 72,787 - EX31_RS17595 0.57 +0.1 72,929 + -0.1 72,929 + -1.3 72,929 + -3.2 72,930 - -0.3 72,942 - -0.7 73,064 + EX31_RS17600 0.27 +0.1 73,077 + EX31_RS17600 0.29 -1.1 73,078 - EX31_RS17600 0.30 +0.9 73,078 - EX31_RS17600 0.30 +0.8 73,078 - EX31_RS17600 0.30 -2.8 73,078 - EX31_RS17600 0.30 +0.1 73,150 + EX31_RS17600 0.43 +0.5 73,196 + EX31_RS17600 0.51 +1.8 73,196 + EX31_RS17600 0.51 +0.3 73,258 + EX31_RS17600 0.63 -0.4 73,258 + EX31_RS17600 0.63 -0.3 73,258 + EX31_RS17600 0.63 +0.7 73,259 - EX31_RS17600 0.63 -1.0 73,259 - EX31_RS17600 0.63 +0.1 73,259 - EX31_RS17600 0.63 -1.4 73,259 - EX31_RS17600 0.63 +0.4
Or see this region's nucleotide sequence